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The map label for this gene is dapE [H]

Identifier: 103485712

GI number: 103485712

Start: 224784

End: 226157

Strand: Reverse

Name: dapE [H]

Synonym: Sala_0216

Alternate gene names: 103485712

Gene position: 226157-224784 (Counterclockwise)

Preceding gene: 103485716

Following gene: 103485701

Centisome position: 6.76

GC content: 67.39

Gene sequence:

>1374_bases
ATGCGCAACCTGTCCCTGCCGCTCGCCCTCGCCGCCGCGCTTGTCGCCGCCGCGCCCGCCCACGCCAAACCCGCCGACGC
CGCAGCCGCGAAGCAGATATTGAAGGACAGCATCGCGATCCCGACGGTCAAGGGCCGCGGCAAGGTGCCCGAACTTGCCG
CCTATTACGCCGGGGTGCTCAAGGCCGCGGGCTATGCCGACGCCGACATCGAAATCACCCCGATGGGCGAGACGGCGACG
CTGGCGGTCACGCTGCGCGGCACGACCGACAAAAAACCCATCCTGCTGCTCGGTCATATGGATGTCGTCGAGGCTGACCC
GAAGGACTGGACGCGCGATCCCTTCCTGCCGGTCGAGGAGGAGGGCTATATTTTCGGGCGCGGGTCGGAAGACAACAAGT
TCGACATCGCGATGATGGTCGCGACGATGGCGCAGCTGAAGCGCGACGGGTTCAAGCCGAAGCGGTCGATCATCCTGCTG
CTGACGGGTGACGAGGAAACCGAAATGGCGACGACGCGTGCGCTTGCCGCCAAGTATCGCAACGCCGAGTTCGCGCTAAA
CGGCGACGGCGGCGGCGGGCTGATCGGCGAAGACGGCAAGCCGCAATACTACAGCCTGCAAGCGGGCGAGAAGACCTATG
CCGATTTCACGCTGGAGGTGACCAATGCCGGCGGCCACAGCTCGCGCCCGTCGCCGGTCAACGCGATCGTTCAGCTTGCC
GATGCCCTGGTCAAGGTCGGCGCCTATCGCTTCCGGCCGCAGGTCAACGAGCTGACCCGGATCGGCCTGCCGATCGTCGC
CGATCGGGTCGGCGGCGAGATCGGGGCGGCGCTGAAGACCTTTGCCGCCGACCCGACCGATACCAAGGCGATCGCGACGA
TTCGCGCCGATCCCGAATATGTCGGGCAGATCGGCACCACCTGCGTGCCGACGCTGGTGAAGGGCGGGCACGCCGAAAAT
GCGCTGCCGCAGCGCGCGACGGCGAACATCAACTGCCGTATCTTTCCCGGCGTCGCGGTCGAGGCGGTGCGTTCCGAGCT
GGAGCGCGTCATCGCCGACCCCGCGGTCAAGGTGCTGACCGATCCCGACGCAAGCGCCAGCGACGCCTCGCCGCTCCGCG
CCGATGTGATGGCGGCGGTGAAGAAAGCCGTCCACGCGCGCGCGCCGGGCCTGCCGATCATCCCGTCGATGAGCGCGGGG
GCGACCGACAGCTATCATTTCCGCATGAACGGCGTGCCGAGTTACGGCGTCGCCGGGCTGTTCTCGAAAGCGAGCGACAG
CTTTGCCCACGGACTCAACGAGCGCGTCCCCGTCGCGGCGATTGCCCCGGCGCTCGCGCATTGGGACAGCCTGCTGCGCG
AGCTTTCGAACTGA

Upstream 100 bases:

>100_bases
GCATCAGTCAAGCCGATTCGGCGATGCCCTTTGCTCGCGAACGGGCAAGGCGGGACATTGGCTCTTGCCGCCATCGGGCG
AAGCCGCTTAACTGCTTTTC

Downstream 100 bases:

>100_bases
CCGGTCAGCCCGCGGCGAGGCGGCGGGCGGTGTCCCGGACGAGCGCGATCATGTTGGGCACGCCCTGCGTGCGGTTCGAC
GAAAGCTGGCGCGTCAGGTC

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRNLSLPLALAAALVAAAPAHAKPADAAAAKQILKDSIAIPTVKGRGKVPELAAYYAGVLKAAGYADADIEITPMGETAT
LAVTLRGTTDKKPILLLGHMDVVEADPKDWTRDPFLPVEEEGYIFGRGSEDNKFDIAMMVATMAQLKRDGFKPKRSIILL
LTGDEETEMATTRALAAKYRNAEFALNGDGGGGLIGEDGKPQYYSLQAGEKTYADFTLEVTNAGGHSSRPSPVNAIVQLA
DALVKVGAYRFRPQVNELTRIGLPIVADRVGGEIGAALKTFAADPTDTKAIATIRADPEYVGQIGTTCVPTLVKGGHAEN
ALPQRATANINCRIFPGVAVEAVRSELERVIADPAVKVLTDPDASASDASPLRADVMAAVKKAVHARAPGLPIIPSMSAG
ATDSYHFRMNGVPSYGVAGLFSKASDSFAHGLNERVPVAAIAPALAHWDSLLRELSN

Sequences:

>Translated_457_residues
MRNLSLPLALAAALVAAAPAHAKPADAAAAKQILKDSIAIPTVKGRGKVPELAAYYAGVLKAAGYADADIEITPMGETAT
LAVTLRGTTDKKPILLLGHMDVVEADPKDWTRDPFLPVEEEGYIFGRGSEDNKFDIAMMVATMAQLKRDGFKPKRSIILL
LTGDEETEMATTRALAAKYRNAEFALNGDGGGGLIGEDGKPQYYSLQAGEKTYADFTLEVTNAGGHSSRPSPVNAIVQLA
DALVKVGAYRFRPQVNELTRIGLPIVADRVGGEIGAALKTFAADPTDTKAIATIRADPEYVGQIGTTCVPTLVKGGHAEN
ALPQRATANINCRIFPGVAVEAVRSELERVIADPAVKVLTDPDASASDASPLRADVMAAVKKAVHARAPGLPIIPSMSAG
ATDSYHFRMNGVPSYGVAGLFSKASDSFAHGLNERVPVAAIAPALAHWDSLLRELSN
>Mature_457_residues
MRNLSLPLALAAALVAAAPAHAKPADAAAAKQILKDSIAIPTVKGRGKVPELAAYYAGVLKAAGYADADIEITPMGETAT
LAVTLRGTTDKKPILLLGHMDVVEADPKDWTRDPFLPVEEEGYIFGRGSEDNKFDIAMMVATMAQLKRDGFKPKRSIILL
LTGDEETEMATTRALAAKYRNAEFALNGDGGGGLIGEDGKPQYYSLQAGEKTYADFTLEVTNAGGHSSRPSPVNAIVQLA
DALVKVGAYRFRPQVNELTRIGLPIVADRVGGEIGAALKTFAADPTDTKAIATIRADPEYVGQIGTTCVPTLVKGGHAEN
ALPQRATANINCRIFPGVAVEAVRSELERVIADPAVKVLTDPDASASDASPLRADVMAAVKKAVHARAPGLPIIPSMSAG
ATDSYHFRMNGVPSYGVAGLFSKASDSFAHGLNERVPVAAIAPALAHWDSLLRELSN

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=450, Percent_Identity=26.2222222222222, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1790395, Length=158, Percent_Identity=34.1772151898734, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322289, Length=338, Percent_Identity=25.4437869822485, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356353, Length=406, Percent_Identity=26.1083743842365, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24649210, Length=335, Percent_Identity=26.5671641791045, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649212, Length=188, Percent_Identity=31.3829787234043, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24649206, Length=144, Percent_Identity=31.9444444444444, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 48055; Mature: 48055

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLSLPLALAAALVAAAPAHAKPADAAAAKQILKDSIAIPTVKGRGKVPELAAYYAGVL
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KAAGYADADIEITPMGETATLAVTLRGTTDKKPILLLGHMDVVEADPKDWTRDPFLPVEE
HHCCCCCCCEEEEECCCCEEEEEEEECCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCC
EGYIFGRGSEDNKFDIAMMVATMAQLKRDGFKPKRSIILLLTGDEETEMATTRALAAKYR
CCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHC
NAEFALNGDGGGGLIGEDGKPQYYSLQAGEKTYADFTLEVTNAGGHSSRPSPVNAIVQLA
CCEEEEECCCCCCCCCCCCCCCEEEECCCCCEECEEEEEEECCCCCCCCCCHHHHHHHHH
DALVKVGAYRFRPQVNELTRIGLPIVADRVGGEIGAALKTFAADPTDTKAIATIRADPEY
HHHHHHHHHHCCCCHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCHHH
VGQIGTTCVPTLVKGGHAENALPQRATANINCRIFPGVAVEAVRSELERVIADPAVKVLT
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEE
DPDASASDASPLRADVMAAVKKAVHARAPGLPIIPSMSAGATDSYHFRMNGVPSYGVAGL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHH
FSKASDSFAHGLNERVPVAAIAPALAHWDSLLRELSN
HHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNLSLPLALAAALVAAAPAHAKPADAAAAKQILKDSIAIPTVKGRGKVPELAAYYAGVL
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KAAGYADADIEITPMGETATLAVTLRGTTDKKPILLLGHMDVVEADPKDWTRDPFLPVEE
HHCCCCCCCEEEEECCCCEEEEEEEECCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCC
EGYIFGRGSEDNKFDIAMMVATMAQLKRDGFKPKRSIILLLTGDEETEMATTRALAAKYR
CCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHC
NAEFALNGDGGGGLIGEDGKPQYYSLQAGEKTYADFTLEVTNAGGHSSRPSPVNAIVQLA
CCEEEEECCCCCCCCCCCCCCCEEEECCCCCEECEEEEEEECCCCCCCCCCHHHHHHHHH
DALVKVGAYRFRPQVNELTRIGLPIVADRVGGEIGAALKTFAADPTDTKAIATIRADPEY
HHHHHHHHHHCCCCHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCHHH
VGQIGTTCVPTLVKGGHAENALPQRATANINCRIFPGVAVEAVRSELERVIADPAVKVLT
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEE
DPDASASDASPLRADVMAAVKKAVHARAPGLPIIPSMSAGATDSYHFRMNGVPSYGVAGL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHH
FSKASDSFAHGLNERVPVAAIAPALAHWDSLLRELSN
HHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA