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The map label for this gene is pspF [H]

Identifier: 103485701

GI number: 103485701

Start: 219907

End: 220932

Strand: Reverse

Name: pspF [H]

Synonym: Sala_0205

Alternate gene names: 103485701

Gene position: 220932-219907 (Counterclockwise)

Preceding gene: 103485712

Following gene: 103485696

Centisome position: 6.6

GC content: 68.03

Gene sequence:

>1026_bases
ATGGAGCGCGAAACGCAATTCATCGGCCAGTCGGCGGCCTTTCAGGACGCGGTCGATCGCGCGAGCCAGGCGGCGCTGCT
CGACCGCCCGGTGCTGGTGATCGGCGAGCGCGGGACGGGCAAGGAACTGATCGCCGAGCGCCTGCACCGTCTGTCGACGC
GCTGGGATCGGCCTTATGTGATCATGAACTGCGCCGCGATGCCCGAGACGCTGATCGAAAGCGAACTGTTCGGACACGAA
GCGGGCGCCTTCACCGGCGCGACGCGCAGCCGCGCGGGACGCTTCGAGGAGGCCGACGGCGGCACGCTCTTTCTCGACGA
ACTGGCGACCATGTCGATGGGCGCGCAGGAACGGCTGCTCCGCGCGGTCGAATATGGCGAGATCACGCGCGTCGGCGCGT
CGCGCCCGATCCGCGTCGATGTGCGCATCGTCGCGGCGACCAACGAGCATCTCCCCGCGCTGGTCGAAGAAAACCGGTTC
AGGGCCGACCTGCTCGACCGGCTGTCGTTCGAGGTCATCACCCTGCCCCCCTTGCGCGCACGCGAAGGCGATATCGAGGT
GCTTGCCACTTACTTCGGGCAGCGCATGGCGGCGGTCGTCGGCTGGGACGAATGGCCCGGATTCGGCCCGCGCGCGCTGG
CGGCGATGGAAGGGCACGACTGGCCGGGCAACGTCCGCGAACTCAGGAACGTCATCGAGCGCGCCGTCTATCGCTGGGGC
GATCCGACGAAGCCGATCGACGCATTGAGCTTCGACCCCTTTGCCAGCCCGTGGCAACCCACGGTGCGCGCGGCGAAGGC
CGAACGTGCGAACGCCGCGGCGTCCGTCGCCGCCCCTCCCGCCGCGCCGTCACCCTCGCCGGGCCCGGTGAGCGACCTGC
GCGCTGCGGTCGACAGCTATGAAAAGGCGATCCTCGCCGACACGATGGCGCGATGCCGCTATAACCAGAAAGTCGCCGCC
GAGGCGCTTGGCCTGAGTTACGACCAGATTCGCCACGCGCTGAAAAAACATGGACTGAATCAATGA

Upstream 100 bases:

>100_bases
ACTATCAAAACTGGCAGAAATGCGGGAAACTTTACGCGCAATTCACTCTCCGCGCTGATTTATCTTGCCAATCATCGGGG
AAATTTGCCAATGGTTGGGA

Downstream 100 bases:

>100_bases
ACCGCGCGCGACGATCCTGACCGGCGACCGCAAGGTCGGGGAGGGGTGACGACGACCCGCGAGCCGCCCCATCTTTCCGT
TCGTGTCGCGAGGCCGGAAA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MERETQFIGQSAAFQDAVDRASQAALLDRPVLVIGERGTGKELIAERLHRLSTRWDRPYVIMNCAAMPETLIESELFGHE
AGAFTGATRSRAGRFEEADGGTLFLDELATMSMGAQERLLRAVEYGEITRVGASRPIRVDVRIVAATNEHLPALVEENRF
RADLLDRLSFEVITLPPLRAREGDIEVLATYFGQRMAAVVGWDEWPGFGPRALAAMEGHDWPGNVRELRNVIERAVYRWG
DPTKPIDALSFDPFASPWQPTVRAAKAERANAAASVAAPPAAPSPSPGPVSDLRAAVDSYEKAILADTMARCRYNQKVAA
EALGLSYDQIRHALKKHGLNQ

Sequences:

>Translated_341_residues
MERETQFIGQSAAFQDAVDRASQAALLDRPVLVIGERGTGKELIAERLHRLSTRWDRPYVIMNCAAMPETLIESELFGHE
AGAFTGATRSRAGRFEEADGGTLFLDELATMSMGAQERLLRAVEYGEITRVGASRPIRVDVRIVAATNEHLPALVEENRF
RADLLDRLSFEVITLPPLRAREGDIEVLATYFGQRMAAVVGWDEWPGFGPRALAAMEGHDWPGNVRELRNVIERAVYRWG
DPTKPIDALSFDPFASPWQPTVRAAKAERANAAASVAAPPAAPSPSPGPVSDLRAAVDSYEKAILADTMARCRYNQKVAA
EALGLSYDQIRHALKKHGLNQ
>Mature_341_residues
MERETQFIGQSAAFQDAVDRASQAALLDRPVLVIGERGTGKELIAERLHRLSTRWDRPYVIMNCAAMPETLIESELFGHE
AGAFTGATRSRAGRFEEADGGTLFLDELATMSMGAQERLLRAVEYGEITRVGASRPIRVDVRIVAATNEHLPALVEENRF
RADLLDRLSFEVITLPPLRAREGDIEVLATYFGQRMAAVVGWDEWPGFGPRALAAMEGHDWPGNVRELRNVIERAVYRWG
DPTKPIDALSFDPFASPWQPTVRAAKAERANAAASVAAPPAAPSPSPGPVSDLRAAVDSYEKAILADTMARCRYNQKVAA
EALGLSYDQIRHALKKHGLNQ

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=338, Percent_Identity=50.5917159763314, Blast_Score=340, Evalue=5e-95,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=51.2931034482759, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788550, Length=335, Percent_Identity=38.2089552238806, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1790437, Length=234, Percent_Identity=49.5726495726496, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1789233, Length=340, Percent_Identity=37.0588235294118, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789087, Length=233, Percent_Identity=49.7854077253219, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082152, Length=344, Percent_Identity=38.3720930232558, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=39.8230088495575, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1786524, Length=233, Percent_Identity=45.4935622317597, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1788905, Length=226, Percent_Identity=41.1504424778761, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1787583, Length=338, Percent_Identity=34.0236686390533, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87081858, Length=215, Percent_Identity=37.2093023255814, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=34.9344978165939, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 37501; Mature: 37501

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERETQFIGQSAAFQDAVDRASQAALLDRPVLVIGERGTGKELIAERLHRLSTRWDRPYV
CCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEE
IMNCAAMPETLIESELFGHEAGAFTGATRSRAGRFEEADGGTLFLDELATMSMGAQERLL
EEECCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHCCHHHHHH
RAVEYGEITRVGASRPIRVDVRIVAATNEHLPALVEENRFRADLLDRLSFEVITLPPLRA
HHHHHCCCEECCCCCCEEEEEEEEEECCCCCCHHHHCCCHHHHHHHHCCCEEEECCCCCC
REGDIEVLATYFGQRMAAVVGWDEWPGFGPRALAAMEGHDWPGNVRELRNVIERAVYRWG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
DPTKPIDALSFDPFASPWQPTVRAAKAERANAAASVAAPPAAPSPSPGPVSDLRAAVDSY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
EKAILADTMARCRYNQKVAAEALGLSYDQIRHALKKHGLNQ
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERETQFIGQSAAFQDAVDRASQAALLDRPVLVIGERGTGKELIAERLHRLSTRWDRPYV
CCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEE
IMNCAAMPETLIESELFGHEAGAFTGATRSRAGRFEEADGGTLFLDELATMSMGAQERLL
EEECCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHCCHHHHHH
RAVEYGEITRVGASRPIRVDVRIVAATNEHLPALVEENRFRADLLDRLSFEVITLPPLRA
HHHHHCCCEECCCCCCEEEEEEEEEECCCCCCHHHHCCCHHHHHHHHCCCEEEECCCCCC
REGDIEVLATYFGQRMAAVVGWDEWPGFGPRALAAMEGHDWPGNVRELRNVIERAVYRWG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
DPTKPIDALSFDPFASPWQPTVRAAKAERANAAASVAAPPAAPSPSPGPVSDLRAAVDSY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
EKAILADTMARCRYNQKVAAEALGLSYDQIRHALKKHGLNQ
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]