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The map label for this gene is menE [H]

Identifier: 103485716

GI number: 103485716

Start: 227943

End: 229475

Strand: Reverse

Name: menE [H]

Synonym: Sala_0220

Alternate gene names: 103485716

Gene position: 229475-227943 (Counterclockwise)

Preceding gene: 103485721

Following gene: 103485712

Centisome position: 6.86

GC content: 65.75

Gene sequence:

>1533_bases
ATGACCAGTCTCGAAATGCTCGACAGCGGCTTTGCCACCCTGCCCGCCCTTGTCCGCGCGCACGCCGCGGAACGTCCCGA
TGCCGTAGCGGCGGCCGACGCAGAACGACGGCTCACCTGGTCCGACCTCGATCGATTGACCGACCGAATCGCCGCGCGCC
TGCAACGGGACGGTTTGGTCAAGGGCGACCGCACGGCGATCGCGGGCCTGAACAGCGTCGAGCAGATGGCGGTGATCCTT
GGCACGCTGCGCGCCGGCGGGGTTGCGGGGCTGATCACCAACAGCGCGACGGGCGAACAGATGGCGGCGATGATCGCCGA
CACCGGGGCGCGCCACCTGTTCCTCGACACGGCGGCGAAGGCGAGCCTCGACGGGCAGGACATCGCCGCGAGCGACCTGA
TCGCGATGGACGGCGGCGACGCGGGCACGCCGCTGACCGACTGGATTGCGCCCGCAGACGCCGCGCCGCAGCCCGCCGCG
ATCGGCCCCGACGACGGTTTCAACATCATCTATTCATCGGGCACCACCGGCACGCCGAAAGGCATCATCCACAGCCATGC
AATGCGCTGGCAGCATATCCAGCGCGGCGCGCCCGCCTATGGCCCGAACGCAGTGACGATCCTGTCGACGCCGCTCTATT
CGAACACGACGATGGCCAGTTTCATGCCAACCGTCGGATCGGGCGGACGGGTCGTCCTGATGAAAAAGTTCGACGCGCGC
GGCTTCCTCGAACTGGCGGAGCGCGAGCGCGCGACCAACTGCATGCTCGTGCCGGTGCAATATCGCCGCATCATGGCGCT
CGACGATTTCGACCGCTTCGACCTTTCGAGCTTCGTGATGAAATACTGCACCTCGGCGCCTTTTCCAGCGGCGCTCAAGG
CCGATGTGCTGAAACGCTGGCCGGGTGGGCTGGTCGAGATTTACGGCATGACCGAAGGCGGCGCGGCCTTCATCCTCGAA
GCGCATCAGTTCCCTGACAAGCTGCACACCGTCGGCAAACCCGCGCCGGGCCATGTCGCCAAGGTGATCGACGAGGACGG
CAACGAACTGCCGCAAGGGTCGGTGGGGGAGATTGTCGGCCGTTCACCCGCAATGATGACCGGGTACAACAACCGCCCCG
ACGCGACGAAGGCGATGCACTGGTACGACAGCGAGGGACAGCTCTTCTATCGCCATGGCGATATCGGGCGGATCGACGAG
GACGGTTTCCTGACGCTGATGGACCGCGCGAAGGACATGATCATTTCGGGTGGCTTCAACATCTTTCCGAGCGACCTTGA
GGCCATTTTGCTCGCCGACGAGCGCGTCGTCGAGGCGGCCGTGGTCGGGATGCCCAGCGAGGAATGGGGCGAAACCCCCG
TCGCCTTCGTCGTGCTGAAACCCGGCGCCGATGCCGAAAGCGTGCGCGAGACGTGCAACGCCAAGGTCGGCAAGACGCAG
CGGCTGAGCGCGATCAGGCAAGTCGACGAACTGCCGCGCAGTCCCATCGGCAAGGTACTGAAACGCGAACTGCGCGACGC
GCATGGCGGATAG

Upstream 100 bases:

>100_bases
CGATTCGTCCCTGCCGGACGCAACGCCGGTCGGCCTGTCCGGCAAGGAAGGGACCGGCGGCGGCGTTGCACCGCCTTTCG
ACTGTGCTAACCCATGCGCC

Downstream 100 bases:

>100_bases
CCGTTCGCGAAGATGGTCGGTGTTGCGGCCGGTTTCGACCGGAAGTGGCCGTTGGCATTTCGGGCTCTCTCCCCTTCAGG
GGAGAGACACGAAGGCTTGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTSLEMLDSGFATLPALVRAHAAERPDAVAAADAERRLTWSDLDRLTDRIAARLQRDGLVKGDRTAIAGLNSVEQMAVIL
GTLRAGGVAGLITNSATGEQMAAMIADTGARHLFLDTAAKASLDGQDIAASDLIAMDGGDAGTPLTDWIAPADAAPQPAA
IGPDDGFNIIYSSGTTGTPKGIIHSHAMRWQHIQRGAPAYGPNAVTILSTPLYSNTTMASFMPTVGSGGRVVLMKKFDAR
GFLELAERERATNCMLVPVQYRRIMALDDFDRFDLSSFVMKYCTSAPFPAALKADVLKRWPGGLVEIYGMTEGGAAFILE
AHQFPDKLHTVGKPAPGHVAKVIDEDGNELPQGSVGEIVGRSPAMMTGYNNRPDATKAMHWYDSEGQLFYRHGDIGRIDE
DGFLTLMDRAKDMIISGGFNIFPSDLEAILLADERVVEAAVVGMPSEEWGETPVAFVVLKPGADAESVRETCNAKVGKTQ
RLSAIRQVDELPRSPIGKVLKRELRDAHGG

Sequences:

>Translated_510_residues
MTSLEMLDSGFATLPALVRAHAAERPDAVAAADAERRLTWSDLDRLTDRIAARLQRDGLVKGDRTAIAGLNSVEQMAVIL
GTLRAGGVAGLITNSATGEQMAAMIADTGARHLFLDTAAKASLDGQDIAASDLIAMDGGDAGTPLTDWIAPADAAPQPAA
IGPDDGFNIIYSSGTTGTPKGIIHSHAMRWQHIQRGAPAYGPNAVTILSTPLYSNTTMASFMPTVGSGGRVVLMKKFDAR
GFLELAERERATNCMLVPVQYRRIMALDDFDRFDLSSFVMKYCTSAPFPAALKADVLKRWPGGLVEIYGMTEGGAAFILE
AHQFPDKLHTVGKPAPGHVAKVIDEDGNELPQGSVGEIVGRSPAMMTGYNNRPDATKAMHWYDSEGQLFYRHGDIGRIDE
DGFLTLMDRAKDMIISGGFNIFPSDLEAILLADERVVEAAVVGMPSEEWGETPVAFVVLKPGADAESVRETCNAKVGKTQ
RLSAIRQVDELPRSPIGKVLKRELRDAHGG
>Mature_509_residues
TSLEMLDSGFATLPALVRAHAAERPDAVAAADAERRLTWSDLDRLTDRIAARLQRDGLVKGDRTAIAGLNSVEQMAVILG
TLRAGGVAGLITNSATGEQMAAMIADTGARHLFLDTAAKASLDGQDIAASDLIAMDGGDAGTPLTDWIAPADAAPQPAAI
GPDDGFNIIYSSGTTGTPKGIIHSHAMRWQHIQRGAPAYGPNAVTILSTPLYSNTTMASFMPTVGSGGRVVLMKKFDARG
FLELAERERATNCMLVPVQYRRIMALDDFDRFDLSSFVMKYCTSAPFPAALKADVLKRWPGGLVEIYGMTEGGAAFILEA
HQFPDKLHTVGKPAPGHVAKVIDEDGNELPQGSVGEIVGRSPAMMTGYNNRPDATKAMHWYDSEGQLFYRHGDIGRIDED
GFLTLMDRAKDMIISGGFNIFPSDLEAILLADERVVEAAVVGMPSEEWGETPVAFVVLKPGADAESVRETCNAKVGKTQR
LSAIRQVDELPRSPIGKVLKRELRDAHGG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=530, Percent_Identity=26.9811320754717, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI58082049, Length=479, Percent_Identity=27.348643006263, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157311624, Length=481, Percent_Identity=27.4428274428274, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157311622, Length=481, Percent_Identity=27.4428274428274, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=24.907063197026, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=24.907063197026, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI42544132, Length=506, Percent_Identity=24.703557312253, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI38505220, Length=372, Percent_Identity=27.6881720430108, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI122937307, Length=505, Percent_Identity=24.3564356435644, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=24.6575342465753, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI38524616, Length=469, Percent_Identity=26.0127931769723, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI195947378, Length=407, Percent_Identity=21.6216216216216, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788107, Length=542, Percent_Identity=27.3062730627306, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=28.6561264822134, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=28.0834914611006, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI221142682, Length=502, Percent_Identity=25.4980079681275, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=23.1053604436229, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786801, Length=455, Percent_Identity=24.1758241758242, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=517, Percent_Identity=27.852998065764, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=28.131021194605, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=526, Percent_Identity=24.3346007604563, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=521, Percent_Identity=25.3358925143954, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=521, Percent_Identity=25.3358925143954, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=521, Percent_Identity=25.3358925143954, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=515, Percent_Identity=25.2427184466019, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=25.5769230769231, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=26.5060240963855, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=503, Percent_Identity=24.8508946322068, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=499, Percent_Identity=25.8517034068136, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71985884, Length=352, Percent_Identity=29.8295454545455, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=496, Percent_Identity=22.3790322580645, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564090, Length=342, Percent_Identity=26.9005847953216, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=342, Percent_Identity=27.4853801169591, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=550, Percent_Identity=24.9090909090909, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355181, Length=484, Percent_Identity=28.099173553719, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=26.6409266409266, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21356947, Length=483, Percent_Identity=27.1221532091097, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24648253, Length=475, Percent_Identity=26.7368421052632, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648255, Length=485, Percent_Identity=26.5979381443299, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24648260, Length=509, Percent_Identity=24.75442043222, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653035, Length=495, Percent_Identity=26.0606060606061, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21356441, Length=500, Percent_Identity=25.8, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21358303, Length=444, Percent_Identity=24.3243243243243, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=22.8675136116152, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076582, Length=501, Percent_Identity=25.1497005988024, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281365686, Length=472, Percent_Identity=26.4830508474576, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130357, Length=490, Percent_Identity=25.5102040816327, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648257, Length=494, Percent_Identity=26.1133603238866, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922652, Length=491, Percent_Identity=24.0325865580448, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=24.4979919678715, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 54630; Mature: 54499

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLEMLDSGFATLPALVRAHAAERPDAVAAADAERRLTWSDLDRLTDRIAARLQRDGLV
CCCHHHHHCCHHHHHHHHHHHHCCCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHCCCC
KGDRTAIAGLNSVEQMAVILGTLRAGGVAGLITNSATGEQMAAMIADTGARHLFLDTAAK
CCCCHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEHHHH
ASLDGQDIAASDLIAMDGGDAGTPLTDWIAPADAAPQPAAIGPDDGFNIIYSSGTTGTPK
CCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
GIIHSHAMRWQHIQRGAPAYGPNAVTILSTPLYSNTTMASFMPTVGSGGRVVLMKKFDAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCEEEEEEECCCC
GFLELAERERATNCMLVPVQYRRIMALDDFDRFDLSSFVMKYCTSAPFPAALKADVLKRW
HHHHHHHHHHCCCEEEEEHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
PGGLVEIYGMTEGGAAFILEAHQFPDKLHTVGKPAPGHVAKVIDEDGNELPQGSVGEIVG
CCCEEEEEEECCCCEEEEEECHHCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHC
RSPAMMTGYNNRPDATKAMHWYDSEGQLFYRHGDIGRIDEDGFLTLMDRAKDMIISGGFN
CCCCEEECCCCCCCHHHEEEEECCCCCEEEEECCCCCCCCCCCEEHHHHHHHEEEECCCC
IFPSDLEAILLADERVVEAAVVGMPSEEWGETPVAFVVLKPGADAESVRETCNAKVGKTQ
CCCCCHHEEEECHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHH
RLSAIRQVDELPRSPIGKVLKRELRDAHGG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSLEMLDSGFATLPALVRAHAAERPDAVAAADAERRLTWSDLDRLTDRIAARLQRDGLV
CCHHHHHCCHHHHHHHHHHHHCCCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHCCCC
KGDRTAIAGLNSVEQMAVILGTLRAGGVAGLITNSATGEQMAAMIADTGARHLFLDTAAK
CCCCHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEHHHH
ASLDGQDIAASDLIAMDGGDAGTPLTDWIAPADAAPQPAAIGPDDGFNIIYSSGTTGTPK
CCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
GIIHSHAMRWQHIQRGAPAYGPNAVTILSTPLYSNTTMASFMPTVGSGGRVVLMKKFDAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCEEEEEEECCCC
GFLELAERERATNCMLVPVQYRRIMALDDFDRFDLSSFVMKYCTSAPFPAALKADVLKRW
HHHHHHHHHHCCCEEEEEHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
PGGLVEIYGMTEGGAAFILEAHQFPDKLHTVGKPAPGHVAKVIDEDGNELPQGSVGEIVG
CCCEEEEEEECCCCEEEEEECHHCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHC
RSPAMMTGYNNRPDATKAMHWYDSEGQLFYRHGDIGRIDEDGFLTLMDRAKDMIISGGFN
CCCCEEECCCCCCCHHHEEEEECCCCCEEEEECCCCCCCCCCCEEHHHHHHHEEEECCCC
IFPSDLEAILLADERVVEAAVVGMPSEEWGETPVAFVVLKPGADAESVRETCNAKVGKTQ
CCCCCHHEEEECHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHH
RLSAIRQVDELPRSPIGKVLKRELRDAHGG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA