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The map label for this gene is amy [H]

Identifier: 103485679

GI number: 103485679

Start: 189531

End: 191333

Strand: Direct

Name: amy [H]

Synonym: Sala_0183

Alternate gene names: 103485679

Gene position: 189531-191333 (Clockwise)

Preceding gene: 103485678

Following gene: 103485680

Centisome position: 5.67

GC content: 65.89

Gene sequence:

>1803_bases
ATGATGCGCCGCCTCGCCGCGTTCGCGCTGCTGCTCGCGCCGCTCCCCGTCGCGGCGGACGAGGCGGCGCTGCGCGTCCG
CCCCGCAAACGACGAGATCATCTATTTCGTGCTTCCCGACCGTTTCGAGAATGGCGATGCGTCGAACGATAGAGGCGGGC
TGAAAGGCGATCGCCTCGCCACCGGCTATGACCCCGCGGCGAAGGGCTTTTTTCACGGCGGCGACCTTGCGGGCCTCACG
AAGCGGCTGGGCTATATCCAGGGGCTCGGCGCCACGGCGATCTGGTTCGCCCCCATTTTCAAGAACAAGCCGGTGCAGGG
CGCGCCGGGGCAGGAAAGCGCGGGCTATCACGGCTATTGGGTGACCGACTTCACGCAGGTCGATCCGCATTTCGGCACGA
ATGCGGAGTTCAAGGCCTTTGTCGACGCGGCGCACGCCCGCGGCATGAAGGTCTATATGGACATCATCGCCAACCACACC
GCCGACGTCATCACCTACAAGGAAGGCGCGGCCACGGATTTCCGCTACCGCAGCCTTGCCGACTATCCCTGGTCGCGCCG
CGGCGGGGTCGATGGCCCGGCGATCAACCCCGGCTTTGCCGGCGACGGGGTCGCGAGTGCGGACAATTGGGCAAAGCTCA
CCGATCCCGCCTTCGCCTACACGCCCGTGGTGCCGAAGGGCGAGGAGCGGGTGAAGGTGCCCGCATGGCTCAACGACCCC
ATTTATTATCACAATCGCGGCAACAGCGACTGGGTGGGCGAATCCGCCCTCTATGGCGACTTTGCCGGGCTCGACGATCT
GGCGACCGAAAATCCGCGTGTGGTGCAGGGCTTCATCGACATCTTCGGTCGCTGGATCGACGAGTTCGGCATCGACGGTT
TCCGCATCGACACCGCCAAACATGTGAACCCGGAGTTCTGGCGCGCCTTCGTCCCCGCGATGCAGGCGCGGGCGAAGGCG
CGCGGTATCCCGAACTTTCACATCTTCGGCGAAGTCTATATCGACGGCGTCGATCCGGGGGCGCTCGCCGCCTGGACGCA
CAGCGCGGGCCTGCCCGCCGTGCTCGATTTCAGCTTCGCGCGCGCGGTGATCGACAGCGTCAGCGGGACCAGGGGCACCG
ACTTGTTCGCGCGGCTGTTCGACGGTGACGTGCTGTACAAGGGCGGCGCCGACGCGGCGCTCCAACTGCCGACCTTCCTC
GGCAATCACGACATGGGCCGCTTTGCGATGTTCGTGAAACAGGCGAACCCGGATGCGGACGACGCCGAACTGCTCGCGCG
GACGATGCTCGGCCATGCGATGCTGCTGACGCTGCGCGGGGTGCCGACCATCTATTATGGCGACGAACAGGGTTTCGTCT
CCGACGGCCACGACCAGCTCGCGCGCGAGGACATGTTCGCGTCAAAGGTTGCCGTCTATAACGACAACGACCTGATCGGC
ACCGACGCGACCACCGCCGATGCCAATTTCGACACCGCGCACCCGCTCTATCGGCATATCGCGGCGCTGGCGGGTATCCG
CCGGAGCACCCCCGCGCTGATGCGCGGCGCAACGAAATTGCGTGCTTTCTCCGACAAGCCCGGTCTGCTCGCGGTGTCGC
GCTTCGACCCCGACACGGGGCGCGAGGTCGTGCTCGCCTATAATACGTCTGCGGCGCCGTTGCGGGCGACGATCTCCGTC
GATATGAAAAGCGCGGCCTTCACCGCGCTCCACGGCGATTGTCCGGCCCGCCCCGTGGCGCCGGGCAGCCTCTCTATTTC
TCTCCCCGCCTTCGGCACGCTCATCTGCCAGGCCAGCGAATAA

Upstream 100 bases:

>100_bases
ATATGGCGGGGATTTCGCTGATGATCGAACGCGAGGTGGCAGTCATGCAAAGTTACGACGACTTCCTTGCCGCGACGCGG
CCCGCCGCGGGGGTCGCGGC

Downstream 100 bases:

>100_bases
GCGATCATAGCGTGACACCCTACCAGCCCCTTGCCCAGGACCAGCTTGCGCCCGCCGCGGATGCCGACACCCCATGGTGG
AAGGGGGCGGCGATCTATCA

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MMRRLAAFALLLAPLPVAADEAALRVRPANDEIIYFVLPDRFENGDASNDRGGLKGDRLATGYDPAAKGFFHGGDLAGLT
KRLGYIQGLGATAIWFAPIFKNKPVQGAPGQESAGYHGYWVTDFTQVDPHFGTNAEFKAFVDAAHARGMKVYMDIIANHT
ADVITYKEGAATDFRYRSLADYPWSRRGGVDGPAINPGFAGDGVASADNWAKLTDPAFAYTPVVPKGEERVKVPAWLNDP
IYYHNRGNSDWVGESALYGDFAGLDDLATENPRVVQGFIDIFGRWIDEFGIDGFRIDTAKHVNPEFWRAFVPAMQARAKA
RGIPNFHIFGEVYIDGVDPGALAAWTHSAGLPAVLDFSFARAVIDSVSGTRGTDLFARLFDGDVLYKGGADAALQLPTFL
GNHDMGRFAMFVKQANPDADDAELLARTMLGHAMLLTLRGVPTIYYGDEQGFVSDGHDQLAREDMFASKVAVYNDNDLIG
TDATTADANFDTAHPLYRHIAALAGIRRSTPALMRGATKLRAFSDKPGLLAVSRFDPDTGREVVLAYNTSAAPLRATISV
DMKSAAFTALHGDCPARPVAPGSLSISLPAFGTLICQASE

Sequences:

>Translated_600_residues
MMRRLAAFALLLAPLPVAADEAALRVRPANDEIIYFVLPDRFENGDASNDRGGLKGDRLATGYDPAAKGFFHGGDLAGLT
KRLGYIQGLGATAIWFAPIFKNKPVQGAPGQESAGYHGYWVTDFTQVDPHFGTNAEFKAFVDAAHARGMKVYMDIIANHT
ADVITYKEGAATDFRYRSLADYPWSRRGGVDGPAINPGFAGDGVASADNWAKLTDPAFAYTPVVPKGEERVKVPAWLNDP
IYYHNRGNSDWVGESALYGDFAGLDDLATENPRVVQGFIDIFGRWIDEFGIDGFRIDTAKHVNPEFWRAFVPAMQARAKA
RGIPNFHIFGEVYIDGVDPGALAAWTHSAGLPAVLDFSFARAVIDSVSGTRGTDLFARLFDGDVLYKGGADAALQLPTFL
GNHDMGRFAMFVKQANPDADDAELLARTMLGHAMLLTLRGVPTIYYGDEQGFVSDGHDQLAREDMFASKVAVYNDNDLIG
TDATTADANFDTAHPLYRHIAALAGIRRSTPALMRGATKLRAFSDKPGLLAVSRFDPDTGREVVLAYNTSAAPLRATISV
DMKSAAFTALHGDCPARPVAPGSLSISLPAFGTLICQASE
>Mature_600_residues
MMRRLAAFALLLAPLPVAADEAALRVRPANDEIIYFVLPDRFENGDASNDRGGLKGDRLATGYDPAAKGFFHGGDLAGLT
KRLGYIQGLGATAIWFAPIFKNKPVQGAPGQESAGYHGYWVTDFTQVDPHFGTNAEFKAFVDAAHARGMKVYMDIIANHT
ADVITYKEGAATDFRYRSLADYPWSRRGGVDGPAINPGFAGDGVASADNWAKLTDPAFAYTPVVPKGEERVKVPAWLNDP
IYYHNRGNSDWVGESALYGDFAGLDDLATENPRVVQGFIDIFGRWIDEFGIDGFRIDTAKHVNPEFWRAFVPAMQARAKA
RGIPNFHIFGEVYIDGVDPGALAAWTHSAGLPAVLDFSFARAVIDSVSGTRGTDLFARLFDGDVLYKGGADAALQLPTFL
GNHDMGRFAMFVKQANPDADDAELLARTMLGHAMLLTLRGVPTIYYGDEQGFVSDGHDQLAREDMFASKVAVYNDNDLIG
TDATTADANFDTAHPLYRHIAALAGIRRSTPALMRGATKLRAFSDKPGLLAVSRFDPDTGREVVLAYNTSAAPLRATISV
DMKSAAFTALHGDCPARPVAPGSLSISLPAFGTLICQASE

Specific function: Since Only Maltooligosaccharides Up To A Chain Length Of 6 Glucose Units Are Actively Transported Through The Cytoplasmic Membrane VIa The Membrane-Bound Complex Of Three Proteins, Malf, Malg, And Malk, Longer Maltooligosaccharides Must First Be Degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=549, Percent_Identity=27.8688524590164, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1790687, Length=124, Percent_Identity=31.4516129032258, Blast_Score=63, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=89, Percent_Identity=38.2022471910112, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583747, Length=544, Percent_Identity=23.7132352941176, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24583749, Length=544, Percent_Identity=23.7132352941176, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586587, Length=229, Percent_Identity=27.0742358078603, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586589, Length=230, Percent_Identity=27.8260869565217, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586593, Length=529, Percent_Identity=23.4404536862004, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583745, Length=232, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586591, Length=231, Percent_Identity=26.4069264069264, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586597, Length=89, Percent_Identity=34.8314606741573, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24586599, Length=89, Percent_Identity=33.7078651685393, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI45549022, Length=88, Percent_Identity=34.0909090909091, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 64785; Mature: 64785

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRLAAFALLLAPLPVAADEAALRVRPANDEIIYFVLPDRFENGDASNDRGGLKGDRLA
CHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEE
TGYDPAAKGFFHGGDLAGLTKRLGYIQGLGATAIWFAPIFKNKPVQGAPGQESAGYHGYW
CCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEHHHCCCCCCCCCCCCCCCCCCEE
VTDFTQVDPHFGTNAEFKAFVDAAHARGMKVYMDIIANHTADVITYKEGAATDFRYRSLA
EECCEECCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHCCCCEEEEECCCCCCCCHHHHHC
DYPWSRRGGVDGPAINPGFAGDGVASADNWAKLTDPAFAYTPVVPKGEERVKVPAWLNDP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEECCEEECCC
IYYHNRGNSDWVGESALYGDFAGLDDLATENPRVVQGFIDIFGRWIDEFGIDGFRIDTAK
EEEECCCCCCCCCCCHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHH
HVNPEFWRAFVPAMQARAKARGIPNFHIFGEVYIDGVDPGALAAWTHSAGLPAVLDFSFA
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHH
RAVIDSVSGTRGTDLFARLFDGDVLYKGGADAALQLPTFLGNHDMGRFAMFVKQANPDAD
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCEEECCHHHCCCCCCHHEEEEECCCCCCC
DAELLARTMLGHAMLLTLRGVPTIYYGDEQGFVSDGHDQLAREDMFASKVAVYNDNDLIG
HHHHHHHHHHHHHHEEHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCEEEEECCCCEEC
TDATTADANFDTAHPLYRHIAALAGIRRSTPALMRGATKLRAFSDKPGLLAVSRFDPDTG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCHHHHHEECCCCCEEEEECCCCCCC
REVVLAYNTSAAPLRATISVDMKSAAFTALHGDCPARPVAPGSLSISLPAFGTLICQASE
CEEEEEECCCCCCEEEEEEEECCHHEEEEECCCCCCCCCCCCCEEEECCCCEEEEEECCC
>Mature Secondary Structure
MMRRLAAFALLLAPLPVAADEAALRVRPANDEIIYFVLPDRFENGDASNDRGGLKGDRLA
CHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEE
TGYDPAAKGFFHGGDLAGLTKRLGYIQGLGATAIWFAPIFKNKPVQGAPGQESAGYHGYW
CCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEHHHCCCCCCCCCCCCCCCCCCEE
VTDFTQVDPHFGTNAEFKAFVDAAHARGMKVYMDIIANHTADVITYKEGAATDFRYRSLA
EECCEECCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHCCCCEEEEECCCCCCCCHHHHHC
DYPWSRRGGVDGPAINPGFAGDGVASADNWAKLTDPAFAYTPVVPKGEERVKVPAWLNDP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEECCEEECCC
IYYHNRGNSDWVGESALYGDFAGLDDLATENPRVVQGFIDIFGRWIDEFGIDGFRIDTAK
EEEECCCCCCCCCCCHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHH
HVNPEFWRAFVPAMQARAKARGIPNFHIFGEVYIDGVDPGALAAWTHSAGLPAVLDFSFA
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHH
RAVIDSVSGTRGTDLFARLFDGDVLYKGGADAALQLPTFLGNHDMGRFAMFVKQANPDAD
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCEEECCHHHCCCCCCHHEEEEECCCCCCC
DAELLARTMLGHAMLLTLRGVPTIYYGDEQGFVSDGHDQLAREDMFASKVAVYNDNDLIG
HHHHHHHHHHHHHHEEHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCEEEEECCCCEEC
TDATTADANFDTAHPLYRHIAALAGIRRSTPALMRGATKLRAFSDKPGLLAVSRFDPDTG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCHHHHHEECCCCCEEEEECCCCCCC
REVVLAYNTSAAPLRATISVDMKSAAFTALHGDCPARPVAPGSLSISLPAFGTLICQASE
CEEEEEECCCCCCEEEEEEEECCHHEEEEECCCCCCCCCCCCCEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]