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The map label for this gene is aglA [H]

Identifier: 103485680

GI number: 103485680

Start: 191345

End: 192988

Strand: Direct

Name: aglA [H]

Synonym: Sala_0184

Alternate gene names: 103485680

Gene position: 191345-192988 (Clockwise)

Preceding gene: 103485679

Following gene: 103485681

Centisome position: 5.72

GC content: 67.21

Gene sequence:

>1644_bases
GTGACACCCTACCAGCCCCTTGCCCAGGACCAGCTTGCGCCCGCCGCGGATGCCGACACCCCATGGTGGAAGGGGGCGGC
GATCTATCAGGTCTATCCGCGCAGCTTTGCCGATTCGAACGGCGACGGTGTGGGCGACCTTGCGGGGATCACCGCAAGGC
TCGACCATATCGCCTCGCTTGGTGTCGATGCGATCTGGCTGTCGCCCTTCTATCCCTCGCCGATGGACGATTTCGGATAC
GACATCGCCGATTATTGCGGTGTCGATCCGATCTTCGGGACGCTCGCCGATTTCGACGCACTGGTGGCGCGCGCGCATGC
GCTGGGGCTGAAGGTCACCACCGACCTCGTGTTCGCGCACACCTCCGACCGGCACGCCTGGTTTGCGGAGAGCCGCGCGA
GCAAGGATAATGACAAGGCCGACTGGTATGTCTGGGCCGACGCGCGGGCGGACGGTTCGCCGCCGACCAACTGGCAGTCG
GTGTTCGGCGGTCCGGCGTGGACGTGGGACGCGCGCCGCGGCCAATATTATATGCACAATTTCCTGAGCTCGCAGCCGCA
GCTCAACGTCCACAACCGCGATGTACAGGACGCGCTGCTCGGCGTCGTGCGCTTCTGGCTCGACCGCGGCGTCGACGGCT
TTCGCATCGACGCGATCAATTTCGCGATGCACGACCCCGAGTTTCGCGACAATCCGCCCGCGCCGCCGTCGAACAAGGTC
CGCACGCGCCCCTTCGATTTCCAGCAGAAAATCTATAACCAGTCGCACCCCGACATTGCGCTCTTCCTCGAACGCATCCG
CGCGCTCACCGACGAGCGCGAGGGGTGCTTCACCGTGGCCGAGGTCGGCGGCGACGATGCGATGCGCGAAATGAAGCTCT
TCACGGCGGGCGAGACGCGCCTCAACAGCGCCTATGGCTTCGACTTTCTCTATGCCGACCGGCTGACGCCGCAACTCGTG
CGCGAGGCGGCCGAGGCATGGCCCGACGCGCCGGGCATCGGCTGGCCGAGCTGGGCGTTCGAGAATCACGACGCGCCGCG
CGCGCTGTCGCGCTGGACTCCGCAAGACGTCGACCGCGACGCCTTTGCGCGCATGAAGATGGCACTGCTCTGCGCGCTCC
GCGGCAATATCATCATCTATAATGGCGAGGAACTGGGGCTCGACCAGGTCGATATTCCCTTCGATCAGGTCAAGGATCCC
GAAGCGCGCAAGAACTGGCCGCTCACCCTGTCGCGCGATGGCGCGCGCACGCCGCTGCCCTGGGCGGCGGCGGCGGCGAA
CGCCGGTTTTTCGGAGGCCGACCCCTGGCTGCCGCTCGGCCCGTCGCACCGCGATCTGGCGGTCGACCGGCAACAGGATG
ATCCCGCGTCGCTGCTCAACCTCACGCGCCGCCTCGTTGCGCTGCGCGCCGCGCACCCCGCGCTCCGCCTCGGCGGCAAC
GCCAACTGGGTGGCCGAGGGCGACCTGCTCGCCTTCGACCGCGTCGCGGGGGAGCAGCGCATTCGCTGTCTTTTCAACTT
CGGCGGCGGCGCGATCGGGATTTCGGCGCTTGCCGACGGCCGCGCGCCGCTGATCGCGCTGAACGGCGCCGACACCGCAC
AGTTGCCGCCATGCGGCGCGCTCTGGCTCAAAATGGAAGGATGA

Upstream 100 bases:

>100_bases
GCGATTGTCCGGCCCGCCCCGTGGCGCCGGGCAGCCTCTCTATTTCTCTCCCCGCCTTCGGCACGCTCATCTGCCAGGCC
AGCGAATAAGCGATCATAGC

Downstream 100 bases:

>100_bases
TCATGCTCCGTCTCGCCCCCCTCGCGCTCCTCGCCGCCGCTGCGGCTCCCGCCGCCGCGCAGCAGGCGCCCGCGCCGGTC
ACCGCGACCTCGCCCGACCG

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTPYQPLAQDQLAPAADADTPWWKGAAIYQVYPRSFADSNGDGVGDLAGITARLDHIASLGVDAIWLSPFYPSPMDDFGY
DIADYCGVDPIFGTLADFDALVARAHALGLKVTTDLVFAHTSDRHAWFAESRASKDNDKADWYVWADARADGSPPTNWQS
VFGGPAWTWDARRGQYYMHNFLSSQPQLNVHNRDVQDALLGVVRFWLDRGVDGFRIDAINFAMHDPEFRDNPPAPPSNKV
RTRPFDFQQKIYNQSHPDIALFLERIRALTDEREGCFTVAEVGGDDAMREMKLFTAGETRLNSAYGFDFLYADRLTPQLV
REAAEAWPDAPGIGWPSWAFENHDAPRALSRWTPQDVDRDAFARMKMALLCALRGNIIIYNGEELGLDQVDIPFDQVKDP
EARKNWPLTLSRDGARTPLPWAAAAANAGFSEADPWLPLGPSHRDLAVDRQQDDPASLLNLTRRLVALRAAHPALRLGGN
ANWVAEGDLLAFDRVAGEQRIRCLFNFGGGAIGISALADGRAPLIALNGADTAQLPPCGALWLKMEG

Sequences:

>Translated_547_residues
MTPYQPLAQDQLAPAADADTPWWKGAAIYQVYPRSFADSNGDGVGDLAGITARLDHIASLGVDAIWLSPFYPSPMDDFGY
DIADYCGVDPIFGTLADFDALVARAHALGLKVTTDLVFAHTSDRHAWFAESRASKDNDKADWYVWADARADGSPPTNWQS
VFGGPAWTWDARRGQYYMHNFLSSQPQLNVHNRDVQDALLGVVRFWLDRGVDGFRIDAINFAMHDPEFRDNPPAPPSNKV
RTRPFDFQQKIYNQSHPDIALFLERIRALTDEREGCFTVAEVGGDDAMREMKLFTAGETRLNSAYGFDFLYADRLTPQLV
REAAEAWPDAPGIGWPSWAFENHDAPRALSRWTPQDVDRDAFARMKMALLCALRGNIIIYNGEELGLDQVDIPFDQVKDP
EARKNWPLTLSRDGARTPLPWAAAAANAGFSEADPWLPLGPSHRDLAVDRQQDDPASLLNLTRRLVALRAAHPALRLGGN
ANWVAEGDLLAFDRVAGEQRIRCLFNFGGGAIGISALADGRAPLIALNGADTAQLPPCGALWLKMEG
>Mature_546_residues
TPYQPLAQDQLAPAADADTPWWKGAAIYQVYPRSFADSNGDGVGDLAGITARLDHIASLGVDAIWLSPFYPSPMDDFGYD
IADYCGVDPIFGTLADFDALVARAHALGLKVTTDLVFAHTSDRHAWFAESRASKDNDKADWYVWADARADGSPPTNWQSV
FGGPAWTWDARRGQYYMHNFLSSQPQLNVHNRDVQDALLGVVRFWLDRGVDGFRIDAINFAMHDPEFRDNPPAPPSNKVR
TRPFDFQQKIYNQSHPDIALFLERIRALTDEREGCFTVAEVGGDDAMREMKLFTAGETRLNSAYGFDFLYADRLTPQLVR
EAAEAWPDAPGIGWPSWAFENHDAPRALSRWTPQDVDRDAFARMKMALLCALRGNIIIYNGEELGLDQVDIPFDQVKDPE
ARKNWPLTLSRDGARTPLPWAAAAANAGFSEADPWLPLGPSHRDLAVDRQQDDPASLLNLTRRLVALRAAHPALRLGGNA
NWVAEGDLLAFDRVAGEQRIRCLFNFGGGAIGISALADGRAPLIALNGADTAQLPPCGALWLKMEG

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=570, Percent_Identity=32.280701754386, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI61744479, Length=134, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI61744481, Length=134, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI65506891, Length=134, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI61744483, Length=134, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI61744477, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI61744475, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790687, Length=485, Percent_Identity=33.8144329896907, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1786604, Length=483, Percent_Identity=23.6024844720497, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32565753, Length=529, Percent_Identity=25.3308128544423, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25147709, Length=481, Percent_Identity=26.8191268191268, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321726, Length=546, Percent_Identity=34.981684981685, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324416, Length=550, Percent_Identity=34.7272727272727, Blast_Score=290, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6322241, Length=550, Percent_Identity=34.7272727272727, Blast_Score=289, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6322021, Length=550, Percent_Identity=34.7272727272727, Blast_Score=289, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6321731, Length=567, Percent_Identity=33.8624338624339, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6319776, Length=567, Percent_Identity=33.8624338624339, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322245, Length=548, Percent_Identity=33.029197080292, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24583745, Length=520, Percent_Identity=37.6923076923077, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24586587, Length=540, Percent_Identity=34.4444444444444, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24586589, Length=512, Percent_Identity=37.5, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24586593, Length=528, Percent_Identity=35.7954545454545, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24586591, Length=522, Percent_Identity=35.4406130268199, Blast_Score=288, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24583747, Length=477, Percent_Identity=37.3165618448637, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24583749, Length=477, Percent_Identity=37.3165618448637, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24586599, Length=547, Percent_Identity=32.5411334552102, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI45549022, Length=522, Percent_Identity=34.2911877394636, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24586597, Length=523, Percent_Identity=33.4608030592734, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI221330053, Length=497, Percent_Identity=35.010060362173, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI281360393, Length=471, Percent_Identity=32.6963906581741, Blast_Score=211, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 60462; Mature: 60331

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPYQPLAQDQLAPAADADTPWWKGAAIYQVYPRSFADSNGDGVGDLAGITARLDHIASL
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHC
GVDAIWLSPFYPSPMDDFGYDIADYCGVDPIFGTLADFDALVARAHALGLKVTTDLVFAH
CCCEEEECCCCCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
TSDRHAWFAESRASKDNDKADWYVWADARADGSPPTNWQSVFGGPAWTWDARRGQYYMHN
CCCCCEEHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEECCCCCHHHHHH
FLSSQPQLNVHNRDVQDALLGVVRFWLDRGVDGFRIDAINFAMHDPEFRDNPPAPPSNKV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCC
RTRPFDFQQKIYNQSHPDIALFLERIRALTDEREGCFTVAEVGGDDAMREMKLFTAGETR
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHEECCCHH
LNSAYGFDFLYADRLTPQLVREAAEAWPDAPGIGWPSWAFENHDAPRALSRWTPQDVDRD
CCCCCCCCEEEHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHH
AFARMKMALLCALRGNIIIYNGEELGLDQVDIPFDQVKDPEARKNWPLTLSRDGARTPLP
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHCCCCCHHCCCCEEEECCCCCCCCC
WAAAAANAGFSEADPWLPLGPSHRDLAVDRQQDDPASLLNLTRRLVALRAAHPALRLGGN
HHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCC
ANWVAEGDLLAFDRVAGEQRIRCLFNFGGGAIGISALADGRAPLIALNGADTAQLPPCGA
CCEEECCCEEEHHHHCCCCEEEEEEECCCCCEEHHHHCCCCCCEEEECCCCCCCCCCCCE
LWLKMEG
EEEEECC
>Mature Secondary Structure 
TPYQPLAQDQLAPAADADTPWWKGAAIYQVYPRSFADSNGDGVGDLAGITARLDHIASL
CCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHC
GVDAIWLSPFYPSPMDDFGYDIADYCGVDPIFGTLADFDALVARAHALGLKVTTDLVFAH
CCCEEEECCCCCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
TSDRHAWFAESRASKDNDKADWYVWADARADGSPPTNWQSVFGGPAWTWDARRGQYYMHN
CCCCCEEHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEECCCCCHHHHHH
FLSSQPQLNVHNRDVQDALLGVVRFWLDRGVDGFRIDAINFAMHDPEFRDNPPAPPSNKV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCC
RTRPFDFQQKIYNQSHPDIALFLERIRALTDEREGCFTVAEVGGDDAMREMKLFTAGETR
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHEECCCHH
LNSAYGFDFLYADRLTPQLVREAAEAWPDAPGIGWPSWAFENHDAPRALSRWTPQDVDRD
CCCCCCCCEEEHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHH
AFARMKMALLCALRGNIIIYNGEELGLDQVDIPFDQVKDPEARKNWPLTLSRDGARTPLP
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHCCCCCHHCCCCEEEECCCCCCCCC
WAAAAANAGFSEADPWLPLGPSHRDLAVDRQQDDPASLLNLTRRLVALRAAHPALRLGGN
HHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCC
ANWVAEGDLLAFDRVAGEQRIRCLFNFGGGAIGISALADGRAPLIALNGADTAQLPPCGA
CCEEECCCEEEHHHHCCCCEEEEEEECCCCCEEHHHHCCCCCCEEEECCCCCCCCCCCCE
LWLKMEG
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]