Methanocaldococcus vulcanius M7

Methanocaldococcus_vulcanius
Names Methanocaldococcus vulcanius M7
Accession numbers NC_013407, NC_013408, NC_013409
Background Methanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7) is an anaerobic, autotrophic, hyperthermophilic methanogen archaeon isolated from a deep-sea hydrothermal chimney sample collected on the East Pacific Rise at a depth of 2600 m. The temperature range for growth at pH 6.5 is 49-89 degrees Celsius, with optimum growth at 80 degrees Celsius. The optimum pH for growth is 6.5, and the optimum NaCl concentration for growth is around 25 g/l. It uses H2 and CO2 as the only substrates for growth and methane production. Tungsten, selenium and yeast extract stimulate growth significantly. In the presence of CO2 and H2, the organism reduces elemental sulphur to hydrogen sulphide. Growth is inhibited by chloramphenicol and rifampicin, but not by ampicillin, kanamycin, penicillin and streptomycin. As determined by 16S rDNA gene sequence analysis, this organism is closely related to Methanococcus jannaschii (strain JAL-1T). However, despite the high percentage of similarity between their 16S rDNA sequences (97.1%), the DNA-DNA hybridization levels between these strains are less than 5%. On the basis of these observations and physiological traits, it is proposed that this organism should be placed in a new species, Methanococcus vulcanius. (Adaptated from PMID: 10319479). (HAMAP: METVM)
Taxonomy
Kingdom:Archaea
Phylum:Euryarchaeota
Class:Methanococci
Order:Methanococcales
Family:Methanocaldococcaceae
Genus:Methanocaldococcus
Species:vulcanius
Strain M7
Complete Yes
Sequencing centre (06-OCT-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
(15-OCT-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method NA
Isolation site Deep-sea hydrothermal chimney sample collected on the East Pacific Rise at a depth of 2600m
Isolation country NA
Number of replicons 3
Gram staining properties Negative
Shape Cocci
Mobility No
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Anaerobic
Optimal temperature 80.0
Temperature range Hyperthermophilic
Habitat Specialized
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Nonsporulating
Metabolism NA
Energy source Autotroph
Diseases NA
Pathogenicity No