Thermococcus sibiricus MM 739

Names Thermococcus sibiricus MM 739
Accession numbers NC_012883
Background Thermococcus sibiricus (strain MM 739 / DSM 12597) is a hyperthermophilic anaerobic archaeon isolated from a well of the never flooded oil-bearing Jurassic horizon of a high-temperature oil reservoir. T. sibiricus indicate the ability to metabolize the buried organic matter from the original oceanic sediment. Its numerous hydrolytic enzymes (e.g., cellulolytic enzymes, agarase, laminarinase, and lipases) and metabolic pathways, support the proposal of the indigenous origin of T. sibiricus in the oil reservoir, and explain its survival over geologic time and its proliferation in this habitat. Indeed, in addition to proteinaceous compounds known previously to be present in oil reservoirs at limiting concentrations, its growth is stimulated by cellulose, agarose, and triacylglycerides, as well as by alkanes. Two polysaccharide degradation loci are probably acquired by T. sibiricus from thermophilic bacteria following lateral gene transfer events. The first, a "saccharolytic gene island" absent in the genomes of other members of the order Thermococcales, contains the complete set of genes responsible for the hydrolysis of cellulose and beta-linked polysaccharides. The second harbors genes for maltose and trehalose degradation. Moreover, T. sibiricus probably possesses a new mechanism of n-alkane degradation, since its growth is stimulated by hexadecane and no enzymes of currently known pathways are encoded. Not reported so far for hyperthermophilic archaea is the lipolytic growth of T. sibiricus on triacylglycerides. This growth is apparently enabled by the function of an extracellular true lipase(s) encoded by the genome. (Adaptated from PMID:19447963). (HAMAP: THESM)
Strain MM 739
Complete Yes
Sequencing centre (19-FEB-2009) Centre 'Bioengineering' RAS, Prosp. 60-let Oktiabria, Bld. 7-1, Moscow 117312, Russia
(29-JUN-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method 454-GS-FLX
Isolation site well of the never flooded oil-bearing Jurassic horizon of a high temperature Samoltor oil reservoir; Russia, Western Siberia
Isolation country Russia
Number of replicons 1
Gram staining properties NA
Shape Cocci
Mobility Yes
Flagellar presence No
Number of membranes 1
Oxygen requirements Anaerobic
Optimal temperature NA
Temperature range Hyperthermophilic
Habitat Hot spring
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Nonsporulating
Metabolism NA
Energy source Organotroph
Diseases NA
Pathogenicity No