Thermococcus gammatolerans EJ3

Thermococcus_gammatolerans
Names Thermococcus gammatolerans EJ3
Accession numbers NC_012804
Background Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) is an obligatory anaerobic heterotrophic hyperthermophilic archaeon isolated from samples collected from hydrothermal chimneys located in the mid-Atlantic Ridge and at the Guyamas basin. T. gammatolerans is obtained by culture enrichment after irradiation with gamma rays at massive doses (30 kGy). It grows optimally at 88 degrees Celsius in the presence of sulfur or cystine on yeast extract, tryptone and peptone, producing H2S. This organism withstands 5 kGy of radiation without any detectable lethality. Exposure to higher doses slightly reduces its viability whereas cell survival of other thermophilic radioresistant archaea drastically decreases when cells are exposed to such radiation doses. Based on these data, T. gammatolerans is one of the most radioresistant archaeon isolated and characterized thus far. An important feature of the T. gammatolerans genome is the absence of genes encoding transposases found in other Archaea, indicating they are not played a role in the evolution of the Thermococcus genomes. The genome of T. gammatolerans contains two virus-related regions, tgv1 (20,832 bp) and tgv2 (20,418 bp). Even if T. gammatolerans is grown with peptides and S, numerous metabolic pathways appear to be active, including those involved in sugar catabolism and amino acid metabolism. (Adaptated from PMID:19558674). (HAMAP: THEGJ)
Taxonomy
Kingdom:Archaea
Phylum:Euryarchaeota
Class:Thermococci
Order:Thermococcales
Family:Thermococcaceae
Genus:Thermococcus
Species:gammatolerans
Strain EJ3
Complete Yes
Sequencing centre (15-JUN-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(22-JAN-2009) Institut de Genetique et Microbiologie, Universite Paris XI / UMR8621 CNRS, Universite Paris-Sud, Centre
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method WGS, BAC clones
Isolation site hydrothermal vent chimneys located in the mid-Atlantic Ridge and Guyamas basin.
Isolation country NA
Number of replicons 1
Gram staining properties NA
Shape Cocci
Mobility No
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Anaerobic
Optimal temperature 88.0
Temperature range Hyperthermophilic
Habitat Specialized
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Nonsporulating
Metabolism NA
Energy source Organotroph
Diseases NA
Pathogenicity No