Brevibacillus brevis NBRC 100599

Names Brevibacillus brevis NBRC 100599
Accession numbers NC_012491
Background Brevibacillus brevis NBRC 100599, formerly known as Bacillus brevis 47, is a strictly aerobic, Gram-positive, spore-forming soil bacterium. It has a characteristic three-layered cell wall composed of a peptidoglycan-layer and the outer two S-layers, each consisting of single protein species, and is known to secrete large amounts of proteins into the culture media. The proteins secreted by this strain are mainly derived from the outer two layers of the cell wall, which are shed into the medium with the progress of cell growth. In secretes little protease, which enables easy recovery of the secreted proteins with minimal degradation, and thus it has been used as a good host for production of heterologous proteins. The genome lacks several sugar metabolism genes found in Bacillus subtilis, showing that B. brevis is unable to utilize L-arabinose, D-mannose and D-xylose. The abundance of genes involved in response to environmental stress, such as those encoding sigma factors and chemotaxis receptors, suggests that this bacterium has a great capacity of adaptation to diverse environments (adapted from (HAMAP: BREBN)
Strain NBRC 100599
Complete Yes
Sequencing centre (01-APR-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(31-MAR-2005) Contact:Director-General Department of Biotechnology National Institute of Technology and Evaluation
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method NA
Isolation site soil
Isolation country Japan
Number of replicons 1
Gram staining properties Positive
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Aerobic
Optimal temperature NA
Temperature range Mesophilic
Habitat Terrestrial
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Sporulating
Metabolism Gramicidin producer
Tyrosidine producer
Energy source NA
Diseases NA
Pathogenicity No