Geobacter sp. FRC-32

Geobacter_sp.
Names Geobacter sp. FRC-32
Accession numbers NC_011979
Background Geobacter sp. (strain FRC-32) is an iron(III)-reducing bacterium isolated from the uranium-contaminated subsurface of the U.S. Department of Energy NABIR Field Research Center (FRC), Oak Ridge, Tennessee. It represents an important environment, the radioactive metal-contaminated subsurface, from which members of the Geobacteraceae have been understudied in pure culture and no genome sequence is available. Several recent studies have verified using cultivation-independent methods that the Geobacteraceae are predominant in subsurface environments where dissimilatory metal reduction is important to the remediation of uranium and other contaminants. Using the 16S rRNA gene as a molecular marker, strain FRC-32 shares 96 % sequence identity to Geobacter bremensis and > 99 % sequence identity to phylotypes detected in the FRC subsurface using cultivation-independent techniques, but it appears to have important physiological differences. Phylotype sequences essentially identical to strain FRC-32 are shown to increase in abundance in parallel with iron(III) and uranium(VI) reduction activity during an in situ bioremediation experiment conducted in the FRC subsurface. Distinct physiological characteristics of strain FRC-32 are that it does not grow on many soluble electron acceptors such as ferric citrate or anthraquinone disulfonate (AQDS). Sequencing the genome of strain FRC-32 will provide further insights into the metabolic capabilities of the Geobacteraceae that may be specific to subsurface members. The genome sequence of strain FRC-32 will aid in the testing of in silico models for the remediation of metal contaminants which have been developed under the Genomes to Life Program using Geobacter strains isolated from surficial aquatic sediments. (adapted from http://genome.jgi-psf.org/finished_microbes/geo_f/geo_f.home.html). (EBI Integr8)
Taxonomy
Kingdom:Bacteria
Phylum:Proteobacteria
Class:Deltaproteobacteria
Order:Desulfuromonadales
Family:Geobacteraceae
Genus:Geobacter
Species:FRC-32
Strain FRC-32
Complete Yes
Sequencing centre (21-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
(27-JAN-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method Sanger
Isolation site Uranium-contaminated subsurface at US
Isolation country NA
Number of replicons 1
Gram staining properties Negative
Shape Bacilli
Mobility No
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Anaerobic
Optimal temperature NA
Temperature range Mesophilic
Habitat Multiple
Biotic relationship Free living
Host name NA
Cell arrangement Singles
Sporulation Nonsporulating
Metabolism Iron reducer
Uranium reducer
Energy source Chemolithotroph
Diseases NA
Pathogenicity No