Methylocella silvestris BL2
Names | Methylocella silvestris BL2 |
---|---|
Accession numbers | NC_011666 |
Background | Methylocella silvestris is an acidophilic, Gram-negative, non-pigmented, non-motile, rod-shaped, aerobic methane-oxidizing bacteria isolated from an acidic forest cambisol (soil characterized by an absence of a layer of accumulated clay, humus, soluble salts, or iron and aluminum oxides) near Marburg, Germany. Unlike other methanotrophs, Methylocella is a catabolically flexible "facultative methanotroph", able to grow on some multicarbon substrates such as acetate, ethanol, pyruvate, succinate, malate and propane. It is able to fix atmospheric nitrogen via an oxygen-sensitive nitrogenase. The cells possess a highly specific bipolar appearance. They lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except Methylocella, but contain a vesicular membrane system connected to the cytoplasmic membrane. A soluble methane monooxygenase was present, but no particulate methane monooxygenase could be detected. These bacteria utilize the serine pathway for carbon assimilation. It is capable of growth at pH values between 4.5 and 7 (with an optimum at pH 5.5) and at temperatures between 4 and 30 degrees C. Compared with Methylocella palustris KT, these strains have greater tolerance of cold temperatures, dissolved salts and methanol (adapted from http://genome.jgi-psf.org/finished_microbes/metsi/metsi.home.html and PubMed 13130000). (EBI Integr8) |
Taxonomy | |
Kingdom: | Bacteria |
Phylum: | Proteobacteria |
Class: | Alphaproteobacteria |
Order: | Rhizobiales |
Family: | Beijerinckiaceae |
Genus: | Methylocella |
Species: | silvestris |
Strain | BL2 |
Complete | Yes |
Sequencing centre | (10-DEC-2008) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA (15-DEC-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA |
Sequencing quality | Level 6: Finished |
Sequencing depth | NA |
Sequencing method | 454-Paired-end, Solexa, Sanger |
Isolation site | Acidic forest cambisol near Marburg Germany |
Isolation country | Germany |
Number of replicons | 1 |
Gram staining properties | Negative |
Shape | Bacilli |
Mobility | No |
Flagellar presence | No |
Number of membranes | 2 |
Oxygen requirements | Aerobic |
Optimal temperature | NA |
Temperature range | Mesophilic |
Habitat | Terrestrial |
Biotic relationship | Free living |
Host name | NA |
Cell arrangement | NA |
Sporulation | NA |
Metabolism | Methane oxidation |
Energy source | Methylotroph |
Diseases | NA |
Pathogenicity | No |
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Selenocompound metabolism
Cyanoamino acid metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Streptomycin biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
Xylene degradation
Chloroalkane and chloroalkene degradation
Glyoxylate and dicarboxylate metabolism
Nitrotoluene degradation
Propanoate metabolism
Ethylbenzene degradation
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Methane metabolism
Carbon fixation in photosynthetic organisms
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Biotin metabolism
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Aminoacyl-tRNA biosynthesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Selenocompound metabolism
Cyanoamino acid metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Streptomycin biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
Xylene degradation
Chloroalkane and chloroalkene degradation
Glyoxylate and dicarboxylate metabolism
Nitrotoluene degradation
Propanoate metabolism
Ethylbenzene degradation
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Methane metabolism
Carbon fixation in photosynthetic organisms
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Biotin metabolism
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Aminoacyl-tRNA biosynthesis