Methylocella silvestris BL2

Names Methylocella silvestris BL2
Accession numbers NC_011666
Background Methylocella silvestris is an acidophilic, Gram-negative, non-pigmented, non-motile, rod-shaped, aerobic methane-oxidizing bacteria isolated from an acidic forest cambisol (soil characterized by an absence of a layer of accumulated clay, humus, soluble salts, or iron and aluminum oxides) near Marburg, Germany. Unlike other methanotrophs, Methylocella is a catabolically flexible "facultative methanotroph", able to grow on some multicarbon substrates such as acetate, ethanol, pyruvate, succinate, malate and propane. It is able to fix atmospheric nitrogen via an oxygen-sensitive nitrogenase. The cells possess a highly specific bipolar appearance. They lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except Methylocella, but contain a vesicular membrane system connected to the cytoplasmic membrane. A soluble methane monooxygenase was present, but no particulate methane monooxygenase could be detected. These bacteria utilize the serine pathway for carbon assimilation. It is capable of growth at pH values between 4.5 and 7 (with an optimum at pH 5.5) and at temperatures between 4 and 30 degrees C. Compared with Methylocella palustris KT, these strains have greater tolerance of cold temperatures, dissolved salts and methanol (adapted from http://genome.jgi-psf.org/finished_microbes/metsi/metsi.home.html and PubMed 13130000). (EBI Integr8)
Taxonomy
Kingdom:Bacteria
Phylum:Proteobacteria
Class:Alphaproteobacteria
Order:Rhizobiales
Family:Beijerinckiaceae
Genus:Methylocella
Species:silvestris
Strain BL2
Complete Yes
Sequencing centre (10-DEC-2008) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
(15-DEC-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method 454-Paired-end, Solexa, Sanger
Isolation site Acidic forest cambisol near Marburg Germany
Isolation country Germany
Number of replicons 1
Gram staining properties Negative
Shape Bacilli
Mobility No
Flagellar presence No
Number of membranes 2
Oxygen requirements Aerobic
Optimal temperature NA
Temperature range Mesophilic
Habitat Terrestrial
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation NA
Metabolism Methane oxidation
Energy source Methylotroph
Diseases NA
Pathogenicity No