Oligotropha carboxidovorans OM5

Names Oligotropha carboxidovorans OM5
Accession numbers NC_011386, NC_015684, NC_015685, NC_015689
Background Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen as an energy source. It was originally isolated from wastewater by enrichment culture studies that were conducted to isolate CO-utilizing bacteria. Originally identified as Pseudomonas carboxidovorans, it was reclassified later as Oligotropha carboxidovorans. No plasmids were found except the megaplasmid pHCG3. The gene for carbon monoxide dehydrogenase, a key enzyme in the utilization of carbon monoxide, is carried on the low-copy-number megaplasmid, which contains also the genes required for chemolithoautotrophic utilization of CO and CO2 (carboxidotrophy), H2 and CO2 (hydrogenotrophy), and CO2 fixation under aerobic conditions. It is also capable of heterotrophic growth under appropriate environmental conditions. O. carboxidovorans is capable of utilizing syngas, a mixture of CO, CO2, and H2 that results from gasification of organic wastes for chemolithoautotrophic growth. Microbial fermentation of this gas mixture could serve as a source for biofuels. The O. carboxidovorans genome sequence will allow future investigations into how it is able to assimilate carbon that is fixed by chemolithoautotrophy into its metabolism, which could benefit the future use of syngas for biofuel production. (EBI Integr8)
Taxonomy
Kingdom:Bacteria
Phylum:Proteobacteria
Class:Alphaproteobacteria
Order:Rhizobiales
Family:Bradyrhizobiaceae
Genus:Oligotropha
Species:carboxidovorans
Strain OM5
Complete Yes
Sequencing centre (22-JUN-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(25-MAY-2011) Goettingen Genomics Laboratory, Georg-August University Goettingen, Institute of Microbiology and
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method 454
Isolation site wastewater enrichment culture
Isolation country Germany
Number of replicons 4
Gram staining properties Negative
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Aerobic
Optimal temperature NA
Temperature range Mesophilic
Habitat Multiple
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation NA
Metabolism NA
Energy source Chemolithoautotroph
Diseases NA
Pathogenicity No