Thermotoga lettingae TMO
Names | Thermotoga lettingae TMO |
---|---|
Accession numbers | NC_009828 |
Background | Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) is an anaerobic, non-spore-forming, mobile, Gram-negative, thermophilic bacterium phylogenetically associated with the Thermotogae. It was isolated from a thermophilic methanol-degrading, sulfate-reducing bioreactor. This organism can convert methanol to CO2 (and partly to alanine) using thiosulfate or elemental sulfur as electron acceptors. The oxidation of methanol is more efficient when Thermotoga lettingae grows in a syntrophic culture with Methanothermobacter thermautotrophicus or Thermodesulfovibrio yellowstonii. The optimum pH, NaCl concentration, and temperature for growth are 7.0, 10%, and 65 degrees Celsius, respectively. In pure culture, strain TMO is also able to ferment methanol to acetate, CO2 and H2. However, this degradation occurs slower than in syntrophic cultures or in the presence of electron acceptors. Besides growing on methanol, strain TMO grows by fermentation on a variety of carbohydrates including monomeric and oligomeric sugars, starch and xylan. Acetate, alanine, CO2, H2, and traces of ethanol, lactate and alpha-aminobutyrate are produced during glucose fermentation. Comparison of 16S rDNA genes reveals that strain TMO is related to Thermotoga subterranea (98%) and Thermotoga elfii (98%). On the basis of the fact that these organisms differ physiologically from strain TMO, it is proposed that strain TMO be classified as a new species, within the genus Thermotoga, as Thermotoga lettingae. (EBI Integr8) |
Taxonomy | |
Kingdom: | Bacteria |
Phylum: | Thermotogae |
Class: | Thermotogae |
Order: | Thermotogales |
Family: | Thermotogaceae |
Genus: | Thermotoga |
Species: | lettingae |
Strain | TMO |
Complete | Yes |
Sequencing centre | (19-SEP-2007) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA (23-AUG-2007) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA |
Sequencing quality | Level 6: Finished |
Sequencing depth | NA |
Sequencing method | Sanger |
Isolation site | Thermophilic sulfate-reducing bioreactor; The Netherlands, Wageningen |
Isolation country | Netherlands |
Number of replicons | 1 |
Gram staining properties | Negative |
Shape | Bacilli |
Mobility | No |
Flagellar presence | Yes |
Number of membranes | 2 |
Oxygen requirements | Anaerobic |
Optimal temperature | 65.0 |
Temperature range | Hyperthermophilic |
Habitat | Specialized |
Biotic relationship | Free living |
Host name | NA |
Cell arrangement | Singles |
Sporulation | Nonsporulating |
Metabolism | Methanol degrading |
Energy source | Heterotroph |
Diseases | NA |
Pathogenicity | No |
Glycolysis / Gluconeogenesis
Pentose phosphate pathway
Galactose metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Lysine biosynthesis
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
Peptidoglycan biosynthesis
One carbon pool by folate
Thiamine metabolism
Pantothenate and CoA biosynthesis
Folate biosynthesis
Terpenoid backbone biosynthesis
Aminoacyl-tRNA biosynthesis
Pentose phosphate pathway
Galactose metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Lysine biosynthesis
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
Peptidoglycan biosynthesis
One carbon pool by folate
Thiamine metabolism
Pantothenate and CoA biosynthesis
Folate biosynthesis
Terpenoid backbone biosynthesis
Aminoacyl-tRNA biosynthesis