Thermotoga lettingae TMO

Names Thermotoga lettingae TMO
Accession numbers NC_009828
Background Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) is an anaerobic, non-spore-forming, mobile, Gram-negative, thermophilic bacterium phylogenetically associated with the Thermotogae. It was isolated from a thermophilic methanol-degrading, sulfate-reducing bioreactor. This organism can convert methanol to CO2 (and partly to alanine) using thiosulfate or elemental sulfur as electron acceptors. The oxidation of methanol is more efficient when Thermotoga lettingae grows in a syntrophic culture with Methanothermobacter thermautotrophicus or Thermodesulfovibrio yellowstonii. The optimum pH, NaCl concentration, and temperature for growth are 7.0, 10%, and 65 degrees Celsius, respectively. In pure culture, strain TMO is also able to ferment methanol to acetate, CO2 and H2. However, this degradation occurs slower than in syntrophic cultures or in the presence of electron acceptors. Besides growing on methanol, strain TMO grows by fermentation on a variety of carbohydrates including monomeric and oligomeric sugars, starch and xylan. Acetate, alanine, CO2, H2, and traces of ethanol, lactate and alpha-aminobutyrate are produced during glucose fermentation. Comparison of 16S rDNA genes reveals that strain TMO is related to Thermotoga subterranea (98%) and Thermotoga elfii (98%). On the basis of the fact that these organisms differ physiologically from strain TMO, it is proposed that strain TMO be classified as a new species, within the genus Thermotoga, as Thermotoga lettingae. (EBI Integr8)
Strain TMO
Complete Yes
Sequencing centre (19-SEP-2007) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(23-AUG-2007) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method Sanger
Isolation site Thermophilic sulfate-reducing bioreactor; The Netherlands, Wageningen
Isolation country Netherlands
Number of replicons 1
Gram staining properties Negative
Shape Bacilli
Mobility No
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Anaerobic
Optimal temperature 65.0
Temperature range Hyperthermophilic
Habitat Specialized
Biotic relationship Free living
Host name NA
Cell arrangement Singles
Sporulation Nonsporulating
Metabolism Methanol degrading
Energy source Heterotroph
Diseases NA
Pathogenicity No