Dehalococcoides sp. BAV1
Names | Dehalococcoides sp. BAV1 |
---|---|
Accession numbers | NC_009455 |
Background | Dehalococcoides sp. (strain BAV1) is an anaerobic bacterium phylogenetically associated with the Chloroflexi. Dehalococcoides sp. (strain BAV1) performs reductive dechlorination and transforms chlorinated ethenes, including vinyl chloride (VC), to innocuous products (i.e., ethene and inorganic chloride. Dehalococcoides sp. (strain BAV1) was isolated from tetrachloroethene-to-ethene-dechlorinating microcosms established with aquifer material collected at the contaminated Bachman Road site in Oscoda, Michigan. Widespread groundwater contamination with chlorinated solvents, particularly chlorinated ethenes, poses environmental and health concerns. All chlorinated ethenes are toxic, and only complete dechlorination results in detoxification. Prior to isolation of strain BAV1, only two bacterial populations were described as capable of dechlorinating tetrachloroethene (PCE) to ethene; Dehalococcoides ethenogenes (strain 195) uses PCE, trichloroethene (TCE) and cis -1,2-dichloroethene (cis-DCE) as metabolic electron acceptors, and Dehalococcoides sp. (strain FL2) grows with TCE, cis-DCE, and trans-1,2-dichloroethene (trans-DCE) . Unfortunately, neither of these two populations grow with VC and the final dechlorination step, VC to ethene, proved to be cometabolic and slow, resulting in VC formation. VC accumulation is particularly troublesome because VC is a human carcinogen. Overcoming the DCE and VC stall is a major obstacle in bioremediation of chlorinated solvent sites. Dehalococcoides sp. (strain BAV1) has been successfully employed in bioremediation at chlorinated solvent sites The genome sequence of strain BAV1 will provide information on the evolution of reductive dehalogenation and the environmentally relevant Dehalococcoides group. The genome will serve as a blueprint for the design of molecular tools to assess and manipulate dechlorination activity at contaminated sites. (EBI Integr8) |
Taxonomy | |
Kingdom: | Bacteria |
Phylum: | Chloroflexi |
Class: | Dehalococcoidetes |
Order: | NA |
Family: | NA |
Genus: | Dehalococcoides |
Species: | BAV1 |
Strain | BAV1 |
Complete | Yes |
Sequencing centre | (01-NOV-2002) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA (03-MAY-2007) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA |
Sequencing quality | Level 6: Finished |
Sequencing depth | NA |
Sequencing method | NA |
Isolation site | a chloroethene-contaminated aquifer |
Isolation country | NA |
Number of replicons | 1 |
Gram staining properties | Negative |
Shape | NA |
Mobility | Yes |
Flagellar presence | No |
Number of membranes | 1 |
Oxygen requirements | Anaerobic |
Optimal temperature | NA |
Temperature range | Mesophilic |
Habitat | Fresh water, Groundwater |
Biotic relationship | Free living |
Host name | NA |
Cell arrangement | NA |
Sporulation | Nonsporulating |
Metabolism | Dechlorinates Tetrachloroethene |
Energy source | Chemolithotroph |
Diseases | NA |
Pathogenicity | No |
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Valine, leucine and isoleucine biosynthesis
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Nitrotoluene degradation
One carbon pool by folate
Riboflavin metabolism
Pantothenate and CoA biosynthesis
Folate biosynthesis
Terpenoid backbone biosynthesis
Aminoacyl-tRNA biosynthesis
Alanine, aspartate and glutamate metabolism
Valine, leucine and isoleucine biosynthesis
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Nitrotoluene degradation
One carbon pool by folate
Riboflavin metabolism
Pantothenate and CoA biosynthesis
Folate biosynthesis
Terpenoid backbone biosynthesis
Aminoacyl-tRNA biosynthesis