Pseudomonas fluorescens SBW25

Names Pseudomonas fluorescens SBW25
Accession numbers NC_009444, NC_012660
Background Pseudomonas fluorescens is a is a physiologically diverse species of opportunistic bacteria that colonizes soil, water and plant surface environments. Some isolates, such as SBW25 and Pf-5, are beneficial to plant health and nutrition. It produces a soluble, greenish fluorescent pigment, particularly under conditions of low iron availability. It is a obligate aerobe, except for some strains that can utilize NO3 as an electron acceptor in place of O2. It is motile by means of multiple polar flagella. It has simple nutritional requirements and grows well in mineral salts media supplemented with any of a large number of carbon sources. Pseudomonas fluorescens strains are being studied for use in applications that require release and survival of bacteria in the soil such as bioremediation of various organic compounds, and biocontrol of pathogens in agriculture. A number of strains suppress plant diseases by protecting the seeds and roots from fungal infection. Competitive exclusion of pathogens as the result of rapid colonization of the rhizosphere by Pseudomonas fluorescens may also be an important factor in disease control.SBW25 was isolated in 1989 from the leaf surface of a sugar beet plant grown at the University Farm, Wytham, Oxford, UK. Comparisons of the 3 available strains (as of June 2009, SBW25, Pf-5 and Pf0-1) indicate they share only 61% of their genes, most of which cluster near the origin of replication. In fact, the three P. fluorescens strains could be different species. 125 SBW25 genes have been shown to be upregulated in the presence of plants; 83 have orthologs in Pf0-1 and 73 have orthologs in Pf-5. Of the 3 strains only SBW25 has plasmid (pQBR103), which was acquired during a field release experiment, however this plasmid is not present in the originally isolated strain. (HAMAP: PSEFS)
Strain SBW25
Complete Yes
Sequencing centre (03-MAY-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(09-JAN-2006) Cerdeno-Tarraga A.M., Pathogen Sequencing Unit, Sanger Institute, Wellcome Trust Genome Campus, Hinxton,
(18-SEP-2008) Cerdeno-Tarraga A.M., Pathogen Sequencing Unit, Sanger Institute, Wellcome Trust Genome Campus, Hinxton,
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method Sanger
Isolation site leaf surfaces of a sugar beet plant
Isolation country NA
Number of replicons 2
Gram staining properties Negative
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Aerobic
Optimal temperature 25.0
Temperature range Mesophilic
Habitat Multiple
Biotic relationship Free living
Host name NA
Cell arrangement Singles
Sporulation Nonsporulating
Metabolism NA
Energy source Heterotroph
Diseases NA
Pathogenicity No
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fructose and mannose metabolism
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Ubiquinone and other terpenoid-quinone biosynthesis
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Geraniol degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Lysine degradation
Arginine and proline metabolism
Histidine metabolism
Tyrosine metabolism
Phenylalanine metabolism
Tryptophan metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
beta-Alanine metabolism
Selenocompound metabolism
Cyanoamino acid metabolism
D-Glutamine and D-glutamate metabolism
D-Alanine metabolism
Glutathione metabolism
Streptomycin biosynthesis
Lipopolysaccharide biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
Toluene degradation
Glyoxylate and dicarboxylate metabolism
Propanoate metabolism
Styrene degradation
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Biotin metabolism
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Caprolactam degradation
Aminoacyl-tRNA biosynthesis