Geobacillus thermodenitrificans NG80-2

Names Geobacillus thermodenitrificans NG80-2
Accession numbers NC_009328, NC_009329
Background Geobacillus thermodenitrificans (strain NG80-2) is a facultative aerobic thermophilic bacterium phylogenetically associated with the Firmicutes. It was isolated from oilfield in Dagang (Northern China) at a depth of 2000 m and a temperature of 73 degrees Celsius. The complete genome sequence consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches (not only in geothermal areas, but also in temperate regions and permanently cold habitats), by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. This strain can use crude oil as a sole carbon source and can degrade 16 to 36 carbon alkanes. It grows between 45 degrees Celsius and 73 degrees Celsius (optimum 65 degrees Celsius), and is capable of oxygen and nitrate respiration. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules. Geobacillus thermodenitrificans NG80-2 also produces an emulsifier, which may be useful for high temperature biodegradation or other industrial purposes. (EBI Integr8)
Taxonomy
Kingdom:Bacteria
Phylum:Firmicutes
Class:Bacilli
Order:Bacillales
Family:Bacillaceae
Genus:Geobacillus
Species:thermodenitrificans
Strain NG80-2
Complete Yes
Sequencing centre (03-APR-2007) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(22-JAN-2007) TEDA School of Biological Sciences and Biotechnology, Nankai University, Hongda Street, TEDA, Tianjin
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method Sanger
Isolation site deep-subsurface oil reservoir, Dagang oilfield, Northern China
Isolation country China
Number of replicons 2
Gram staining properties Positive
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Facultative
Optimal temperature 65.0
Temperature range Thermophilic
Habitat Specialized
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Sporulating
Metabolism Degrades long-chain alkanes
Hydrocarbon degrading
Energy source Chemoorganotroph
Diseases NA
Pathogenicity No