Geobacillus thermodenitrificans NG80-2
Names | Geobacillus thermodenitrificans NG80-2 |
---|---|
Accession numbers | NC_009328, NC_009329 |
Background | Geobacillus thermodenitrificans (strain NG80-2) is a facultative aerobic thermophilic bacterium phylogenetically associated with the Firmicutes. It was isolated from oilfield in Dagang (Northern China) at a depth of 2000 m and a temperature of 73 degrees Celsius. The complete genome sequence consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches (not only in geothermal areas, but also in temperate regions and permanently cold habitats), by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. This strain can use crude oil as a sole carbon source and can degrade 16 to 36 carbon alkanes. It grows between 45 degrees Celsius and 73 degrees Celsius (optimum 65 degrees Celsius), and is capable of oxygen and nitrate respiration. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules. Geobacillus thermodenitrificans NG80-2 also produces an emulsifier, which may be useful for high temperature biodegradation or other industrial purposes. (EBI Integr8) |
Taxonomy | |
Kingdom: | Bacteria |
Phylum: | Firmicutes |
Class: | Bacilli |
Order: | Bacillales |
Family: | Bacillaceae |
Genus: | Geobacillus |
Species: | thermodenitrificans |
Strain | NG80-2 |
Complete | Yes |
Sequencing centre | (03-APR-2007) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA (22-JAN-2007) TEDA School of Biological Sciences and Biotechnology, Nankai University, Hongda Street, TEDA, Tianjin |
Sequencing quality | Level 6: Finished |
Sequencing depth | NA |
Sequencing method | Sanger |
Isolation site | deep-subsurface oil reservoir, Dagang oilfield, Northern China |
Isolation country | China |
Number of replicons | 2 |
Gram staining properties | Positive |
Shape | Bacilli |
Mobility | Yes |
Flagellar presence | Yes |
Number of membranes | 1 |
Oxygen requirements | Facultative |
Optimal temperature | 65.0 |
Temperature range | Thermophilic |
Habitat | Specialized |
Biotic relationship | Free living |
Host name | NA |
Cell arrangement | NA |
Sporulation | Sporulating |
Metabolism | Degrades long-chain alkanes Hydrocarbon degrading |
Energy source | Chemoorganotroph |
Diseases | NA |
Pathogenicity | No |
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Pentose and glucuronate interconversions
Fructose and mannose metabolism
Galactose metabolism
Fatty acid biosynthesis
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Geraniol degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Starch and sucrose metabolism
Amino sugar and nucleotide sugar metabolism
Peptidoglycan biosynthesis
Pyruvate metabolism
Glyoxylate and dicarboxylate metabolism
Propanoate metabolism
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Pantothenate and CoA biosynthesis
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Aminoacyl-tRNA biosynthesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Pentose and glucuronate interconversions
Fructose and mannose metabolism
Galactose metabolism
Fatty acid biosynthesis
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Geraniol degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Starch and sucrose metabolism
Amino sugar and nucleotide sugar metabolism
Peptidoglycan biosynthesis
Pyruvate metabolism
Glyoxylate and dicarboxylate metabolism
Propanoate metabolism
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Pantothenate and CoA biosynthesis
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Aminoacyl-tRNA biosynthesis