Caulobacter crescentus CB15

Names Caulobacter crescentus CB15
Accession numbers NC_002696
Background Caulobacter crescentus is a single-celled, small bacterium that divides asymmetrically producing cells that differ in structure and function - it is an ideal model system for the mechanisms of asymmetric cell division and has been studied thoroughly.The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes. It was found that the C. crescentus genome encodes for more two-component signal transduction proteins that assist in cell cycle progression than any other bacterial genome sequenced so far. Caulobacter also uses DNA methylation as a regulatory mechanism in cell cycle progression. Caulobacter also has several genes and clusters of genes that are essential for survival in habitats with poor nutrients. Some of these genes encode for proteins involved in chemotaxis, outer membrane channel function, degradation of compounds with aromatic rings, and the breakdown of plant-derived carbon sources (list from Nierman et al. 2001). Others are involved in extracytoplasmic function sigma factors, which allow the bacterium to respond to "a wide range of environmental fluctuations" (Nierman et al. 2001).Caulobacter are Gram-negative, rod-like cells that can be flagellated in a polar manner or have a stalk. Caulobacter lack intracellular organelles. They are hereotrophic aerobes and can be found in aquatic environments attached to particulate matter, plant materials, or other microorganisms by its stalk. Caulobacter asymmetrically divides to produce two types of daughter cells that are functionally and structurally different. The cylindrical body of a stalk cell is approximately 0.7 micrometers in diameter and 2-3 micrometers in length. The swarmer cell is smaller than the stalk cell initially; the different hydrodynamics due to the different morphologies between the swarmer and stalk cells allow swarmers to be isolated relatively easily (Stanford).Caulobacter generally live in a dilute aquatic environment where the most common limiting nutrient is phosphorus, an essential element for healthy growth. Lack of this nutrient induce Caulobacter to dramatically elongate its stalk up to 30 times longer than those in phosphate-rich medium (Brun et al. 2000).Caulobacter asymmetrically divides to produce a motile swarmer cell and a stalk cell. The swarmer cell, which has a flagellum, swims for about 30-45 minutes before shedding the flagellum and differentiating into a stalk cell. Within the swarmer cell, chromosomes do not replicate; however, chromosome replication begins immediately in the daughter cell with the stalk and when the swarmer loses its tail (Stanford). The stalk adheres to surfaces through an adhesive organelle called the holdfast.Several two-component signal transduction proteins are involved in the cell cycle progression by accumulating at one or both poles "in a spatial and temporal pattern that is reproduced during each cycle" (Jacobs-Wagner 2003). In addition to this, DNA methylation is main component of signaling differentiation. Throughout the cell cycle, the chromosome progressively goes from being fully methylated to hemimethylated during DNA replication - this results in differential binding of regulatory proteins to activate or repress transcription. This was studied by using the CtrA gene, which encodes for an important cell cycle regulatory protein. The CtrA gene has two promoters, one of which "fires early" in the S phase and is recognized by the CcrM DNA methyltransferase. Analysis showed that this P1 promoter is actually repressed by DNA methylation (Reinsenauer and Shapiro 2002).In order to analyze the cell cycle regulatory mechanisms and other mechanisms of Caulobacter, many mutant strains have been made and studied. These fascinating mutants are a hands-on way to analyze genes. Stalk synthesis, for example, is greatly affected by the amount of phosphate in the bacterium's environment. These stalks, generally 1 micrometer in length, grow up to 20 to 30 micrometers in length in response to phosphate starvation. The increased surface/volume ratio is though to enhance nutrient uptake. One type of mutant, the skl mutant, has increased stalk enlongation even in phosphate-rich medium. This type of mutant maps to homologues of Pst genes that, in E. coli and other bacteria, encode for high-affinity phosphate transport proteins. These skl mutants allow expression of PhoB in Caulobacter uncharacteristic of wild-type cells in phosphate-rich medium. It was also found that this same phoB gene is necessary for stalk elongation during phosphate starvation because it encodes for the transcriptional activator of the Pho regulon. In E. coli, PhoB acivates or represses the transcription of pho regulon genes by binding to Pho boxes in the promoters of pho regulon genes. In E. coli and Pseudomonoas aeruginosa, it has been shown that pst mutants can no longer repress PhoR, thus inducing the Pho regulon and inducing the formation of an elongated stalk (Brun et al. 2000). (From (MicrobeWiki: Caulobacter)
Strain CB15
Complete Yes
Sequencing centre (28-MAR-2001) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(31-JAN-2001) The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, MD 20850, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method NA
Isolation site pond water
Isolation country USA
Number of replicons 1
Gram staining properties Negative
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Aerobic
Optimal temperature 35.0
Temperature range Mesophilic
Habitat Aquatic
Biotic relationship Free living
Host name NA
Cell arrangement Singles
Sporulation NA
Metabolism NA
Energy source NA
Diseases NA
Pathogenicity No