Polaromonas sp. JS666
| Names | Polaromonas sp. JS666 |
|---|---|
| Accession numbers | NC_007948, NC_007949, NC_007950 |
| Background | Polaromonas (strain JS666 / ATCC BAA-500), a member of the family Comamonadaceae in the beta-proteobacteria, is a novel, aerobic, cis-dichloroethene (cDCE)-assimilating organism with optimum growth at 20-25 degrees C. It is closely related to the Antarctic marine isolate Polaromonas vacuolata. The substantial phylogenetic distance from other known aerobic alkene-assimilating bacteria suggests a novel biochemistry for cDCE oxidation. Polaromonas is the only aerobic organism known to use cDCE for energy and growth. cDCE is a common groundwater contaminant derived mainly from incomplete anaerobic reductive dechlorination of the widely used chlorinated solvents tetrachloroethene and trichloroethene. The toxicity and suspected carcinogenicity of cDCE qualifies it as an EPA priority pollutant. Since growth-coupled oxidation of cDCE does not appear to be common at field sites, JS666 is a prime candidate for bioremediation at sites where cDCE has migrated into aerobic zones. Recently, a Polaromonas was reported to be the organism responsible for in situ biodegradation of naphthalene at a coal-tar-contaminated site. Closely related strains have also been found recently in a variety of contaminated sites, but their roles are unknown. The recent isolation of the above strains suggests that members of the genus Polaromonas play a major role in the subsurface degradation of environmental contaminants that has been overlooked to date because of an emphasis on mesophilic bacteria. (EBI Integr8) |
| Taxonomy | |
| Kingdom: | Bacteria |
| Phylum: | Proteobacteria |
| Class: | Betaproteobacteria |
| Order: | Burkholderiales |
| Family: | Comamonadaceae |
| Genus: | Polaromonas |
| Species: | JS666 |
| Strain | NA |
| Complete | Yes |
| Sequencing centre | (03-APR-2006) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA (11-APR-2006) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA |
| Sequencing quality | Level 6: Finished |
| Sequencing depth | NA |
| Sequencing method | Sanger |
| Isolation site | Sediment contaminated with cis-dichloroethane |
| Isolation country | NA |
| Number of replicons | 3 |
| Gram staining properties | Negative |
| Shape | Bacilli |
| Mobility | No |
| Flagellar presence | No |
| Number of membranes | 2 |
| Oxygen requirements | Aerobic |
| Optimal temperature | 20.0 |
| Temperature range | Mesophilic |
| Habitat | Multiple |
| Biotic relationship | Free living |
| Host name | NA |
| Cell arrangement | NA |
| Sporulation | NA |
| Metabolism | NA |
| Energy source | NA |
| Diseases | None |
| Pathogenicity | No |
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Geraniol degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine metabolism
Benzoate degradation
Tryptophan metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
beta-Alanine metabolism
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Glutathione metabolism
Streptomycin biosynthesis
Lipopolysaccharide biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
Xylene degradation
Chloroalkane and chloroalkene degradation
Naphthalene degradation
Glyoxylate and dicarboxylate metabolism
Nitrotoluene degradation
Propanoate metabolism
Ethylbenzene degradation
Styrene degradation
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Carbon fixation in photosynthetic organisms
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Biotin metabolism
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Caprolactam degradation
Aminoacyl-tRNA biosynthesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Geraniol degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine metabolism
Benzoate degradation
Tryptophan metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
beta-Alanine metabolism
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Glutathione metabolism
Streptomycin biosynthesis
Lipopolysaccharide biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
Xylene degradation
Chloroalkane and chloroalkene degradation
Naphthalene degradation
Glyoxylate and dicarboxylate metabolism
Nitrotoluene degradation
Propanoate metabolism
Ethylbenzene degradation
Styrene degradation
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Carbon fixation in photosynthetic organisms
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Biotin metabolism
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Caprolactam degradation
Aminoacyl-tRNA biosynthesis