Escherichia coli O157:H7 str. Sakai

Names Escherichia coli O157:H7 str. Sakai
Accession numbers NC_002127, NC_002128, NC_002695
Background Escherichia coli is a Gram-negative straight rod, which either uses peritrichous flagella for mobility or is nonmotile. It is a facultatively anaerobic chemoorganotroph capable of both respiratory and fermentative metabolism. E.coli serves a useful function in the body by suppressing the growth of harmful bacterial species and by synthesising appreciable amounts of vitamins. It is an important component of the biosphere. It colonizes the lower gut of animals and survives when released to the natural environment, allowing widespread dissemination to new hosts. Pathogenic E.coli strains are responsible for infection of the enteric, urinary, pulmonary and nervous systems. Comparison of 20 E.coli/Shigella strains shows the core genome to be about 2000 genes while the pan-genome has over 18,000 genes. There are multiple, striking integration hotspots that are conserved across the genomes, corresponding to regions of abundant and parallel insertions and deletions of genetic material.This strain is an avian pathogenic E.coli (APEC), and was isolated from the lung of a chicken with colisepticemia. E.coli APEC O1 is an O1:K1:H7 strain belonging to phylogroup B2 and was chosen for sequencing as it possesses traits characteristics of E.coli which cause disease outside of the intestinal tract i.e. APEC and UPEC (uropathogenic E.coli) strains. It is highly virulent in chickens. It is closely related to E.coli UTI89, a UPEC strain of E.coli (ECOUT). It contains 4 plasmids, pAPEC-O1-ColBM, pAPEC-O1-R, pAPEC-O1-Cryptic1 and pAPEC-O1-Cryptic2. Plasmid pAPEC-O1-ColBM is an F-type plasmid that produces colicins B and M and encodes a putative virulence cluster. Plasmid pAPEC-O1-R encodes resistance to eight antimicrobial agents. The cryptic plasmids are somewhat related to Yersinia-type plasmids and do not confer any apparent phenotypes. (HAMAP: ECOK1)
Strain O157:H7
Complete Yes
Sequencing centre (10-SEP-2004) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(25-JUN-2001) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method NA
Isolation site Patient in 1922
Isolation country NA
Number of replicons 3
Gram staining properties Negative
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Facultative
Optimal temperature 37.0
Temperature range Mesophilic
Habitat HostAssociated
Biotic relationship Free living
Host name Homo sapiens
Cell arrangement Pairs, Singles
Sporulation Nonsporulating
Metabolism NA
Energy source NA
Diseases Hemorrhagic colitis
Pathogenicity NA
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Pentose and glucuronate interconversions
Fructose and mannose metabolism
Galactose metabolism
Ascorbate and aldarate metabolism
Fatty acid metabolism
Ubiquinone and other terpenoid-quinone biosynthesis
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
beta-Alanine metabolism
Taurine and hypotaurine metabolism
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Alanine metabolism
Glutathione metabolism
Starch and sucrose metabolism
Amino sugar and nucleotide sugar metabolism
Streptomycin biosynthesis
Lipopolysaccharide biosynthesis
Peptidoglycan biosynthesis
Glycerolipid metabolism
Glycerophospholipid metabolism
Pyruvate metabolism
Glyoxylate and dicarboxylate metabolism
Nitrotoluene degradation
Propanoate metabolism
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Biotin metabolism
Lipoic acid metabolism
Folate biosynthesis
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Aminoacyl-tRNA biosynthesis
Biosynthesis of siderophore group nonribosomal peptides