Agrobacterium sp. H13-3

Names Agrobacterium sp. H13-3
Accession numbers NC_015183, NC_015184, NC_015508
Background Agrobacterium are Gram-negative, motile, soil-dwelling plant pathogens with the species name given based on the disease phenotype associated with the bacteria. They invade the crown, roots and stems of a great variety of plants via wounds causing tumors. The diseases are crown gall, hairy root, and cane gall. Some strains possess a wide host range, whereas other possess a very limited host range. The tumor is correlated with the presence of a large tumor-inducing plasmid (Ti plasmid) in the bacteria. Thus A.tumefaciens causes crown gall on many dicotyledonous plants; A.rubi causes crown gall on raspberries; A.vitis gall formation on grapes; A.rhizogenes causes hairy roots; A.radiobacter is avirulent. However the ability to cause disease is associated with transmissible plasmids, and this grouping is easily disrupted when plasmids move from one strain to another. More recently Agrobacterium have been classified into 3 biovars based on physiological and biochemical phenotypes without consideration of disease; the 2 classification systems are not compatible.Agrobacterium sp. H13-3, formerly known as Rhizobium lupini strain H13-3, was isolated from the rhizosphere of Lupinus luteus, the European yellow lupin, in Hungary in 1957. It has been used to study flagellum structure and chemotaxis. Sequencing has identified it as a genomic species G1 strain of the biovar I cluster. It lacks a Ti tumor-inducing plasmid, and so is non-pathogenic. It does not nodulate L.luteus under laboratory conditions. Despite being unable to induce tumors it is more competent for opine utilization than the tumorigenic A.tumefaciens C58 strain (AGTR5), and also encodes more iron acquisition systems (adapted from PMID 21329740). (HAMAP: AGRSH)
Strain H13-3
Complete Yes
Sequencing centre (01-OCT-2010) Center for Biotechnology (CeBiTec), Bielefeld University, Universitaetstrasse 27, Bielefeld, NRW 33594,
(02-MAR-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method 454-GS-FLX
Isolation site rhizosphere of Lupinus luteus
Isolation country NA
Number of replicons 3
Gram staining properties Negative
Shape NA
Mobility Yes
Flagellar presence Yes
Number of membranes 2
Oxygen requirements NA
Optimal temperature NA
Temperature range NA
Habitat HostAssociated
Biotic relationship NA
Host name NA
Cell arrangement NA
Sporulation NA
Metabolism NA
Energy source NA
Diseases NA
Pathogenicity No