Arthrobacter phenanthrenivorans Sphe3

Names Arthrobacter phenanthrenivorans Sphe3
Accession numbers NC_015145, NC_015146, NC_015147
Background Arthrobacter phenanthrenivorans (strain DSM 18606 / JCM 16027 / LMG 23796 / Sphe3) is an aerobic, non-motile Gram-positive bacterium isolated from Perivleptos, a creosote polluted site in Epirus, Greece (12 Km North of the city of Ioannina), where a wood preserving industry was operating for over 30 years. A. phenanthrenivorans is of particular interest because it is able to metabolize phenanthrene at concentrations of up to 400 mg/L as a sole source of carbon and energy, at rates faster than those reported for other Arthrobacter species. It appears to internalize phenanthrene with two mechanisms: a passive diffusion when cells are grown on glucose, and an inducible active transport system, when cells are grown on phenanthrene as a sole carbon source. A. phenanthrenivorans exhibits a rod-coccus cycle, with a cell size of approximately 1.0-1.5 x 2.5-4.0 um. Colonies are slightly yellowish on Luria agar. The temperature range is between 40-37 degrees Celsius with optimum growth between 30-37 degrees Celsius. The pH range is between 6.5-8.5 with optimal growth at pH 7.0-7.5. it is found to be sensitive to various antibiotics: ampicillin (20 mg/L), chloramphenicol (10 mg/L), erythromycin (10 mg/L), neomycin (20 mg/L), rifampicin (10 mg/L) and tetracycline (10 mg/L). (Adapted from: (EBI Integr8)
Strain Sphe3
Complete Yes
Sequencing centre (10-DEC-2010) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
(17-FEB-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method Sanger, 454
Isolation site Creosote polluted soil at Perivleptos (Epirus, Greece)
Isolation country Greece
Number of replicons 3
Gram staining properties Positive
Shape Bacilli
Mobility No
Flagellar presence No
Number of membranes 1
Oxygen requirements Aerobic
Optimal temperature 30.0
Temperature range Mesophilic
Habitat Terrestrial
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Nonsporulating
Metabolism PAH-degrading
Energy source NA
Diseases None
Pathogenicity No