Bacillus thuringiensis BMB171

Names Bacillus thuringiensis BMB171
Accession numbers NC_014171, NC_014172
Background Bacillus thuringiensis is a Gram-positive, spore-forming bacterium that belongs to the B.cereus group along with Bacillus anthracis and Bacillus cereus. Bacillus thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture due to its various endogenous crystal proteins and spores. When insects ingest toxin crystals, the alkaline pH of their digestive tract activates the toxin. Cry inserts into the insect gut cell membrane, forming a pore. The pore results cell lysis and eventual death of the insect.B.thuringiensis is not amenable to genetic engineering due to its thick cell wall layer, which along with the natural presence of many plasmids reduces its transformation efficiency. Strain BMB171 is a laboratory derivative of wild-type crystalliferous strain YBT- 1463. It has a high transformation efficiency and has only 1 plasmid. While it no longer encodes crystal protein genes it is able to produce these proteins upon transformation with appropriate plasmids (adapted from PMID 20525827 and Wikipedia). (HAMAP: BACT1)
Strain BMB171
Complete Yes
Sequencing centre (01-FEB-2010) State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Road,
(20-MAY-2010) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method 454-GS-FLX
Isolation site NA
Isolation country NA
Number of replicons 2
Gram staining properties Positive
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Microaerophilic
Optimal temperature NA
Temperature range Mesophilic
Habitat HostAssociated
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation NA
Metabolism NA
Energy source NA
Diseases NA
Pathogenicity No