Thermosphaera aggregans DSM 11486

Names Thermosphaera aggregans DSM 11486
Accession numbers NC_014160
Background Thermosphaera aggregans (strain DSM 11486 / M11TL) is a strictly anaerobic, hyperthermophile archaeum isolated from water and sediment samples of a terrestrial circumneutral hot solfataric spring ("Obsidian Pool") located in the Mud Volcano area of the Yellowstone National Park, Wyoming. It is restricted to hot, pH neutral, terrestrial springs. T. aggregans is a regular coccus that preferentially grows in grape-like aggregates consisting of five to several hundred individuals. It grows optimally at 85 degrees Celsius, and the temperature range for growth is 67 to 90 degrees Celsius. The pH range for growth is 5.0-7.0 with an optimum at pH 6.5. T. aggregans grows optimally in the absence of exogenous NaCl, but can be adapted to salt concentrations of up to 0.7%. Upon growth on yeast extract and peptone, the fermentation products acetate, isovalerate, CO(2) and H(2) are identified, but no growth on meat extract, amylose, glycogen, cellulose, cellobiose, maltose, raffinose, pyruvate and acetate is observed. Growth is inhibited by sulfur and H(2). Interestingly, an inhibiting effect in cultures of T. aggregans is not observed, if growth media are supplemented with the sulfur compounds sulfide, sulfite or thiosulfate, so that this effect seems to be restricted to elemental sulfur. The inhibiting effect of H(2) on growth is reversible and can be explained by a product inhibition of sensitive hydrogenases, which may be required for the disposal of reducing equivalents as hydrogen during fermentation. (Adapted from : (HAMAP: THEAM)
Strain DSM 11486
Complete Yes
Sequencing centre (16-FEB-2010) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA
(17-MAY-2010) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method Illumina, 454-GS-FLX-Titanium
Isolation site Hot spring from Obsidian Pool at Yellowstone Natl. Park
Isolation country USA
Number of replicons 1
Gram staining properties NA
Shape Cocci
Mobility Yes
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Anaerobic
Optimal temperature NA
Temperature range Hyperthermophilic
Habitat Hot spring, Solfataric field
Biotic relationship Free living
Host name NA
Cell arrangement Chains, Pairs, Singles
Sporulation Nonsporulating
Metabolism NA
Energy source Heterotroph
Diseases NA
Pathogenicity No