Azospirillum sp. B510

Names Azospirillum sp. B510
Accession numbers NC_013854, NC_013855, NC_013856, NC_013857, NC_013858, NC_013859, NC_013860
Background Azospirillum sp. B510 is a rice endophyte isolated in August 1999 from the surface-sterilized stems of rice plants (Oryza sativa cv. Nipponbare) cultivated in an experimental paddy field in Japan. In addition to being a diazotroph under free-living conditions, B510 was found to have positive motility, and to be capable of degrading plant cell walls. B510 is capable of colonizing internal rice tissues, and inoculation with B510 was shown to promote plant growth under both laboratory and field conditions. It is capable of fixing N(2) in planta. B510 inoculation increases stem number resulting in an increase in seed yield. The genome consists of 1 chromosome and 6 plasmids, 2 of which encode essential genes. There are 280 insertions sequences and at least 2 putative prophages in the genome. Three putative plant hormone-related genes encoding tryptophan 2-monooxytenase (iaaM) and indole-3-acetaldehyde hydrolase (iaaH), which are involved in indole-3-actic acid biosynthesis, and 1-aminocyclopropane-1-carboxylate deaminase (acdS), which reduces ethylene levels, were identified. Moreover, B510 inoculation enhanced disease resistance to virulent rice blast fungus and the bacterial pathogen Xanthomonas oryzae.15 Thus, Azospirillum sp. B510 is likely a beneficial bacterium with agronomic applications (adapted from PMID 20047946). (EBI Integr8)
Strain B510
Complete Yes
Sequencing centre (17-FEB-2010) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(22-DEC-2008) Contact:Shusei Sato Kazusa DNA Research Institute, Department of Plant Genome Research
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method BAC clones, WGS
Isolation site rice plants (Oryza sativa cv. Nipponbare)
Isolation country Japan
Number of replicons 7
Gram staining properties Negative
Shape Bacilli
Mobility No
Flagellar presence Yes
Number of membranes 2
Oxygen requirements Facultative
Optimal temperature 34.0
Temperature range Mesophilic
Habitat Terrestrial
Biotic relationship Symbiotic
Host name NA
Cell arrangement NA
Sporulation Nonsporulating
Metabolism Nitrogen fixation
Energy source NA
Diseases NA
Pathogenicity No
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Pentose and glucuronate interconversions
Galactose metabolism
Fatty acid metabolism
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Arginine and proline metabolism
Histidine metabolism
Phenylalanine metabolism
Benzoate degradation
Fluorobenzoate degradation
Phenylalanine, tyrosine and tryptophan biosynthesis
Taurine and hypotaurine metabolism
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Glutathione metabolism
Streptomycin biosynthesis
Lipopolysaccharide biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
Toluene degradation
Glyoxylate and dicarboxylate metabolism
Nitrotoluene degradation
Propanoate metabolism
Ethylbenzene degradation
Styrene degradation
Butanoate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Riboflavin metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Lipoic acid metabolism
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Nitrogen metabolism
Sulfur metabolism
Caprolactam degradation
Aminoacyl-tRNA biosynthesis