Bacillus pseudofirmus OF4

Names Bacillus pseudofirmus OF4
Accession numbers NC_013791, NC_013792, NC_013793
Background This facultatively alkaliphilic Bacillus (it will grow at pH 7.5 and 10.5 on malate-containing medium) is the best studied of the alkaliphile Bacillus. Oxidative phosphorylation supported by the proton-coupled ATP synthase of alkaliphilic Bacillus species at pH 10.5 presents a set of challenges with respect to capture of protons, successful proton translocation to the cytoplasm, and proton retention in the cytoplasm. B. pseudofirmus OF4 maintains a cytoplasmic pH of 8.2 during growth in rigorously controlled continuous culture at pH 10.5 in medium containing malate, a non-fermentable substrate. A Na+ cycle plays a central role in the capacity of these aerobic, extremely alkaliphilic Bacillus species for pH homeostasis. The capacity for pH homeostasis, in turn, appears to set the upper pH limit for growth. One limb of the alkaliphile Na+ cycle consists of Na+/H+ antiporters that achieve net H+ accumulation that is coupled to Na+ efflux (modified from PMID 11248197 and 19176524). (EBI Integr8)
Strain OF4
Complete Yes
Sequencing centre (04-JAN-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
(15-DEC-2010) Center for Genomic Sciences, Allegheny-Singer Research Institute, 320 E. North Ave. 11th Floor,
(25-JAN-2010) Center for Genomic Sciences, Allegheny-Singer Research Institute, 320 E. North Ave. 11th Floor,
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method NA
Isolation site soil in New York State by alkaline enrichment cultures
Isolation country USA
Number of replicons 3
Gram staining properties Positive
Shape Bacilli
Mobility Yes
Flagellar presence Yes
Number of membranes 1
Oxygen requirements Facultative
Optimal temperature NA
Temperature range Mesophilic
Habitat Terrestrial
Biotic relationship Free living
Host name NA
Cell arrangement NA
Sporulation Sporulating
Metabolism NA
Energy source NA
Diseases NA
Pathogenicity No