Listeria monocytogenes 08-5923

Listeria_monocytogenes
Names Listeria monocytogenes 08-5923
Accession numbers NC_013768
Background Gram-positive rods motile by a few peritrichous flagella. 1-10% of humans might be intestinal carriers of L.monocytogenes. It has been found in at least 37 mammalian species, both domestic and feral, as well as at least 17 species of birds and possibly some species of fish and shellfish. It can be isolated from soil, silage, and other environmental sources. L.monocytogenes is quite hardy and resists the deleterious effects of freezing, drying, and heat remarkably well for a bacterium that does not form spores. The manifestations of listeriosis include septicemia, meningitis (or meningoencephalitis), encephalitis, and intrauterine or cervical infections in pregnant women. Most listeriosis is caused by serotypes 4b, 1/2a and 1/2b, while serotype 4a is naturally avirulent, and hence has possibilities as a vaccine candidate.This is one of 2 strains sequenced during a nationwide outbreak of listeriosis associated with ready-to-eat meat products in Canada during the summer of 2008; it was isolated from human blood. The outbreak resulted in 22 deaths and at least 57 illnesses. The 2 genomes are colinear with differences including 27 single nucleotide polymorphisms (SNPs) and three indels, including a 33 kb prophage. The SNPs observed in the genome of this isolate included 20 non-synonymous, 5 synonymous and 2 intergenic mutations. This predominance of non-synonymous changes suggests that it was under strong positive selection for functional divergence rather than purifying selection. The two isolates were found to harbor a 50 kb putative mobile genomic island encoding translocation and efflux functions that has not been observed in other Listeria genomes (adapted from PMID 20167121). (HAMAP: LISM2)
Taxonomy
Kingdom:Bacteria
Phylum:Firmicutes
Class:Bacilli
Order:Bacillales
Family:Listeriaceae
Genus:Listeria
Species:monocytogenes
Strain 08-5923
Complete Yes
Sequencing centre (09-APR-2009) National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba
(27-JAN-2010) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Sequencing quality Level 6: Finished
Sequencing depth NA
Sequencing method 454-GS-FLX
Isolation site clinical isolate during a nationwide outbreak
Isolation country Canada
Number of replicons 1
Gram staining properties Positive
Shape Bacilli
Mobility Yes
Flagellar presence Yes?
Number of membranes 1
Oxygen requirements Facultative
Optimal temperature NA
Temperature range Mesophilic
Habitat HostAssociated
Biotic relationship Free living
Host name Homo sapiens
Cell arrangement Chains, Singles
Sporulation Nonsporulating
Metabolism NA
Energy source Chemoorganotroph
Diseases NA
Pathogenicity Yes