Listeria monocytogenes 08-5923
Names | Listeria monocytogenes 08-5923 |
---|---|
Accession numbers | NC_013768 |
Background | Gram-positive rods motile by a few peritrichous flagella. 1-10% of humans might be intestinal carriers of L.monocytogenes. It has been found in at least 37 mammalian species, both domestic and feral, as well as at least 17 species of birds and possibly some species of fish and shellfish. It can be isolated from soil, silage, and other environmental sources. L.monocytogenes is quite hardy and resists the deleterious effects of freezing, drying, and heat remarkably well for a bacterium that does not form spores. The manifestations of listeriosis include septicemia, meningitis (or meningoencephalitis), encephalitis, and intrauterine or cervical infections in pregnant women. Most listeriosis is caused by serotypes 4b, 1/2a and 1/2b, while serotype 4a is naturally avirulent, and hence has possibilities as a vaccine candidate.This is one of 2 strains sequenced during a nationwide outbreak of listeriosis associated with ready-to-eat meat products in Canada during the summer of 2008; it was isolated from human blood. The outbreak resulted in 22 deaths and at least 57 illnesses. The 2 genomes are colinear with differences including 27 single nucleotide polymorphisms (SNPs) and three indels, including a 33 kb prophage. The SNPs observed in the genome of this isolate included 20 non-synonymous, 5 synonymous and 2 intergenic mutations. This predominance of non-synonymous changes suggests that it was under strong positive selection for functional divergence rather than purifying selection. The two isolates were found to harbor a 50 kb putative mobile genomic island encoding translocation and efflux functions that has not been observed in other Listeria genomes (adapted from PMID 20167121). (HAMAP: LISM2) |
Taxonomy | |
Kingdom: | Bacteria |
Phylum: | Firmicutes |
Class: | Bacilli |
Order: | Bacillales |
Family: | Listeriaceae |
Genus: | Listeria |
Species: | monocytogenes |
Strain | 08-5923 |
Complete | Yes |
Sequencing centre | (09-APR-2009) National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba (27-JAN-2010) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA |
Sequencing quality | Level 6: Finished |
Sequencing depth | NA |
Sequencing method | 454-GS-FLX |
Isolation site | clinical isolate during a nationwide outbreak |
Isolation country | Canada |
Number of replicons | 1 |
Gram staining properties | Positive |
Shape | Bacilli |
Mobility | Yes |
Flagellar presence | Yes? |
Number of membranes | 1 |
Oxygen requirements | Facultative |
Optimal temperature | NA |
Temperature range | Mesophilic |
Habitat | HostAssociated |
Biotic relationship | Free living |
Host name | Homo sapiens |
Cell arrangement | Chains, Singles |
Sporulation | Nonsporulating |
Metabolism | NA |
Energy source | Chemoorganotroph |
Diseases | NA |
Pathogenicity | Yes |
Glycolysis / Gluconeogenesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fructose and mannose metabolism
Fatty acid biosynthesis
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Taurine and hypotaurine metabolism
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Streptomycin biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Aminoacyl-tRNA biosynthesis
Citrate cycle (TCA cycle)
Pentose phosphate pathway
Fructose and mannose metabolism
Fatty acid biosynthesis
Synthesis and degradation of ketone bodies
Purine metabolism
Pyrimidine metabolism
Alanine, aspartate and glutamate metabolism
Cysteine and methionine metabolism
Valine, leucine and isoleucine degradation
Valine, leucine and isoleucine biosynthesis
Lysine biosynthesis
Histidine metabolism
Phenylalanine, tyrosine and tryptophan biosynthesis
Taurine and hypotaurine metabolism
Selenocompound metabolism
D-Glutamine and D-glutamate metabolism
D-Arginine and D-ornithine metabolism
D-Alanine metabolism
Streptomycin biosynthesis
Peptidoglycan biosynthesis
Pyruvate metabolism
C5-Branched dibasic acid metabolism
One carbon pool by folate
Thiamine metabolism
Vitamin B6 metabolism
Nicotinate and nicotinamide metabolism
Pantothenate and CoA biosynthesis
Folate biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
Aminoacyl-tRNA biosynthesis