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The map label for this gene is cysS

Identifier: 99080994

GI number: 99080994

Start: 1243613

End: 1244986

Strand: Direct

Name: cysS

Synonym: TM1040_1153

Alternate gene names: 99080994

Gene position: 1243613-1244986 (Clockwise)

Preceding gene: 99080993

Following gene: 99080995

Centisome position: 38.85

GC content: 60.26

Gene sequence:

>1374_bases
ATGACGACGATCAAATTGCACAACTCAAAGACCCGCAAGAAAGAGGTCTTTACGCCGATTGATCCCGACAATGTGCAAAT
GTATGTCTGTGGCCCAACGGTTTATGATCGCGCGCATCTTGGGAATGCCCGCCCGGTGGTGGTGTTTGACGTTCTGTTTC
GCCTGCTGCGCCACGTCTATGGCGACAAGAAAGTCACCTACGCGCGCAATTTCACCGACGTCGATGACAAGATCAACACT
CGCGCCGCCGAAAGCGGGCGCGCAATTGGCGAGATCACCTCGGAGACGATCCAGTGGTTCCTTGAGGATATGGGCGCGCT
TGGGGCGCTGCAGCCCACGCATATGCCACGCGCCACGGAATACATCCCGCAGATGATCGCCATGATCGAAGAGTTGATCG
CGCGCGATCACGCCTATGAAGCCGAGGGGCATGTGCTCTTTGCGGTGGACTCCTGGCGCGAAGGGTATGGGCAGCTCTCG
GGACGGTCGGTTGACGATATGATCGCTGGCGCGCGGGTTGAGGTGGCACCCTACAAGAAAAACCCGATGGATTTTGTGCT
CTGGAAGCCGTCGGATGCCGACCAGCCGGGCTGGGAGAGCCCATGGGGGCGGGGACGGCCGGGCTGGCACATCGAATGCT
CTGCCATGAGTCATGATCTCTTGGGCGAGAGTTTTGATATTCATTGCGGTGGCAATGACCTGATGTTCCCGCACCATGAA
AACGAGATCGCCCAGAGCTGCTGTGCCAACCCCGAAGGCGAGTTTGCGCGCTATTGGCTGCACAATGAGATGCTGCAGGT
TGAGGGCAAGAAAATGTCTAAATCGCTGGGCAATTTCTTTACCGTGCGCGATCTCCTGGATCAGGGAATCCCGGGCGAGG
TGGTGCGATTTGTGTTCCTGCAGACCCACTATCGAAAGCCGATGGACTGGACCGAGAAGAAAGCCGCCGAGGCGGAGGCG
ACGTTGCGCAAGTGGCGGGCGCTGACAGCGGGCATCGAGCCCGCCGCCACCGCAGCTGCCGCGGTTGTGGACGCGCTGTC
GGATGATCTCAATACCGCAGGGGCGATTGCCGAGATGCACAAGCTGGCGGCGGCTGGCGATGGTGCAGGGCTTTTGGCGG
CTGGCCAACTGGTTGGCCTTTTGGGCGATGAGATGGGCGAATGGGCTGCTGCGCCCTCGGTGGATCTCTCGGCCCATGAG
GCGCGCCTGTTTAAGGCACGCCAGGCGGCCATGGAGACCAAGGATTTCTCCGAGGTGGATCGCCTGAAGGCCGCCTATAC
CGAGGCAGGGCTTGAGGTGCGCATGTCCAAAACCGGGGTGGAATTGGTCCCGGGCGCAGGCTTTGACGCTGCAAAGCTGG
AGGCGCTGTCGTGA

Upstream 100 bases:

>100_bases
TCTCTTTCTTGTGACAAGTTGCTAAGCCCGCGCTACGCGCAGTGCGCTTGCTGTGGTGCTTGGGATTTGCCCGCGCTGAC
TTGTTGTCGAAGGAGCTGAC

Downstream 100 bases:

>100_bases
CGGAACGGCTCTATCTTTATGACACCACCCTGCGCGACGGTCAGCAGACCCAAGGGGTGCAATTCTCCACGCTTGAAAAA
AAGCAGATCGCCCAGGCCCT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTTIKLHNSKTRKKEVFTPIDPDNVQMYVCGPTVYDRAHLGNARPVVVFDVLFRLLRHVYGDKKVTYARNFTDVDDKINT
RAAESGRAIGEITSETIQWFLEDMGALGALQPTHMPRATEYIPQMIAMIEELIARDHAYEAEGHVLFAVDSWREGYGQLS
GRSVDDMIAGARVEVAPYKKNPMDFVLWKPSDADQPGWESPWGRGRPGWHIECSAMSHDLLGESFDIHCGGNDLMFPHHE
NEIAQSCCANPEGEFARYWLHNEMLQVEGKKMSKSLGNFFTVRDLLDQGIPGEVVRFVFLQTHYRKPMDWTEKKAAEAEA
TLRKWRALTAGIEPAATAAAAVVDALSDDLNTAGAIAEMHKLAAAGDGAGLLAAGQLVGLLGDEMGEWAAAPSVDLSAHE
ARLFKARQAAMETKDFSEVDRLKAAYTEAGLEVRMSKTGVELVPGAGFDAAKLEALS

Sequences:

>Translated_457_residues
MTTIKLHNSKTRKKEVFTPIDPDNVQMYVCGPTVYDRAHLGNARPVVVFDVLFRLLRHVYGDKKVTYARNFTDVDDKINT
RAAESGRAIGEITSETIQWFLEDMGALGALQPTHMPRATEYIPQMIAMIEELIARDHAYEAEGHVLFAVDSWREGYGQLS
GRSVDDMIAGARVEVAPYKKNPMDFVLWKPSDADQPGWESPWGRGRPGWHIECSAMSHDLLGESFDIHCGGNDLMFPHHE
NEIAQSCCANPEGEFARYWLHNEMLQVEGKKMSKSLGNFFTVRDLLDQGIPGEVVRFVFLQTHYRKPMDWTEKKAAEAEA
TLRKWRALTAGIEPAATAAAAVVDALSDDLNTAGAIAEMHKLAAAGDGAGLLAAGQLVGLLGDEMGEWAAAPSVDLSAHE
ARLFKARQAAMETKDFSEVDRLKAAYTEAGLEVRMSKTGVELVPGAGFDAAKLEALS
>Mature_456_residues
TTIKLHNSKTRKKEVFTPIDPDNVQMYVCGPTVYDRAHLGNARPVVVFDVLFRLLRHVYGDKKVTYARNFTDVDDKINTR
AAESGRAIGEITSETIQWFLEDMGALGALQPTHMPRATEYIPQMIAMIEELIARDHAYEAEGHVLFAVDSWREGYGQLSG
RSVDDMIAGARVEVAPYKKNPMDFVLWKPSDADQPGWESPWGRGRPGWHIECSAMSHDLLGESFDIHCGGNDLMFPHHEN
EIAQSCCANPEGEFARYWLHNEMLQVEGKKMSKSLGNFFTVRDLLDQGIPGEVVRFVFLQTHYRKPMDWTEKKAAEAEAT
LRKWRALTAGIEPAATAAAAVVDALSDDLNTAGAIAEMHKLAAAGDGAGLLAAGQLVGLLGDEMGEWAAAPSVDLSAHEA
RLFKARQAAMETKDFSEVDRLKAAYTEAGLEVRMSKTGVELVPGAGFDAAKLEALS

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=390, Percent_Identity=38.7179487179487, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI21269877, Length=224, Percent_Identity=39.2857142857143, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI62240992, Length=224, Percent_Identity=39.2857142857143, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI10835051, Length=224, Percent_Identity=39.2857142857143, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI303304987, Length=224, Percent_Identity=39.2857142857143, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1786737, Length=367, Percent_Identity=47.4114441416894, Blast_Score=330, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI17509795, Length=317, Percent_Identity=31.8611987381703, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17509797, Length=317, Percent_Identity=31.8611987381703, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324082, Length=272, Percent_Identity=36.0294117647059, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI19922218, Length=379, Percent_Identity=35.8839050131926, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI19922364, Length=310, Percent_Identity=34.1935483870968, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_SILST (Q1GHI0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613148.1
- ProteinModelPortal:   Q1GHI0
- SMR:   Q1GHI0
- STRING:   Q1GHI0
- GeneID:   4078449
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1153
- NMPDR:   fig|292414.1.peg.3712
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   SSP292414:TM1040_1153-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50367; Mature: 50235

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKLHNSKTRKKEVFTPIDPDNVQMYVCGPTVYDRAHLGNARPVVVFDVLFRLLRHVY
CCEEEECCCCCCHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHH
GDKKVTYARNFTDVDDKINTRAAESGRAIGEITSETIQWFLEDMGALGALQPTHMPRATE
CCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
YIPQMIAMIEELIARDHAYEAEGHVLFAVDSWREGYGQLSGRSVDDMIAGARVEVAPYKK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHCCCCCCHHHHHCCCEEEECCCCC
NPMDFVLWKPSDADQPGWESPWGRGRPGWHIECSAMSHDLLGESFDIHCGGNDLMFPHHE
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCCEEEEECCCCCCCCCCH
NEIAQSCCANPEGEFARYWLHNEMLQVEGKKMSKSLGNFFTVRDLLDQGIPGEVVRFVFL
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QTHYRKPMDWTEKKAAEAEATLRKWRALTAGIEPAATAAAAVVDALSDDLNTAGAIAEMH
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAAAGDGAGLLAAGQLVGLLGDEMGEWAAAPSVDLSAHEARLFKARQAAMETKDFSEVD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
RLKAAYTEAGLEVRMSKTGVELVPGAGFDAAKLEALS
HHHHHHHHCCCEEEEECCCCEEECCCCCCHHHHCCCC
>Mature Secondary Structure 
TTIKLHNSKTRKKEVFTPIDPDNVQMYVCGPTVYDRAHLGNARPVVVFDVLFRLLRHVY
CEEEECCCCCCHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHH
GDKKVTYARNFTDVDDKINTRAAESGRAIGEITSETIQWFLEDMGALGALQPTHMPRATE
CCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
YIPQMIAMIEELIARDHAYEAEGHVLFAVDSWREGYGQLSGRSVDDMIAGARVEVAPYKK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHCCCCCCHHHHHCCCEEEECCCCC
NPMDFVLWKPSDADQPGWESPWGRGRPGWHIECSAMSHDLLGESFDIHCGGNDLMFPHHE
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCCEEEEECCCCCCCCCCH
NEIAQSCCANPEGEFARYWLHNEMLQVEGKKMSKSLGNFFTVRDLLDQGIPGEVVRFVFL
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QTHYRKPMDWTEKKAAEAEATLRKWRALTAGIEPAATAAAAVVDALSDDLNTAGAIAEMH
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAAAGDGAGLLAAGQLVGLLGDEMGEWAAAPSVDLSAHEARLFKARQAAMETKDFSEVD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
RLKAAYTEAGLEVRMSKTGVELVPGAGFDAAKLEALS
HHHHHHHHCCCEEEEECCCCEEECCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA