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The map label for this gene is queA

Identifier: 99080934

GI number: 99080934

Start: 1171133

End: 1172182

Strand: Direct

Name: queA

Synonym: TM1040_1093

Alternate gene names: 99080934

Gene position: 1171133-1172182 (Clockwise)

Preceding gene: 99080933

Following gene: 99080935

Centisome position: 36.59

GC content: 63.33

Gene sequence:

>1050_bases
TTGAAACTGGGCGATTTTGATTTTGACCTGCCAGAAGAGCTGATCGCGACACGCCCTGCCGTGCCGCGCTCTTCGGCGCG
TCTTCTGGTGGCAGAGCAGGACCGGATTTCGGACGGGCGCGTCACCGACCTTTTGGACTGGCTGGCACCGGGGGACCGGC
TGGTGCTCAATGACACCCGCGTGATCCCTGCGCGTCTCAGCGGTCAGCGCCACCGCAACTCAGCGCAGGGACCGGTCTCG
GCACGCATCGAGGTAACGCTTCTGGAGCCGCAGGCCGATGGCAGCTGGGCGGGTCTCCTGAAGCCCCTGAAAAAAATCAA
GATCGGCGAGACCCTGCGGTTCTCCGATGCGCTTTCGGCCGAGCTTGTCGATGTGGTCGATGGTCAGGGACGGCTTCGGT
TCAACCTCGCGGGCGATGATTTTGACGCGGCGCTGGCAGAGGCGGGCGCGATGCCCCTGCCGCCCTATATCGCCGCCAAA
CGCCCCGCCGACGAGCAGGACAAGACTGACTATCAAACCGTCTGGGCGCGCAATTCCGGCGCGGTGGCGGCGCCCACCGC
GAGCCTTCATTTTGACGAGCCGCTCCTGAAGGCGCTGTCCGAGAAGGGGGTGACCTTTACCTATGTGACCCTGCATGTGG
GCGCGGGCACCTTCCTGCCCGTGAAGGTCGAGGATGTGACCACCCACAAGATGCACGCCGAATGGGGCCAGGTGACGCCG
GAGGCCGCCGCCGAGATTGCAGCCACCAAGGCCGCAGGCAATCGGGTGATCCCCGTGGGCACCACGGCGCTGCGCCTGAT
CGAAAGCGCGGCACGCGAAAGCGGCGCCATCACACCCTGGGAAGGCGAGACGGACATTTTTATCTATCCGGGTTTCGAGT
TCCGGGTGGCTGATGCGTTGATGACGAATTTTCATCTGCCAAAATCGACCCTGATGATGCTGGTCTCTGCCCTGATGGGG
CAGGATCGCATCCGCGCGATCTATGCGCATGCGGTAAAGGAGCGGTATCGCTTCTTTTCCTACGGGGATGCCTCGCTGCT
CATTCCCTGA

Upstream 100 bases:

>100_bases
AGGTGGCACGACGAACGGCAATCCGCCCGGCAAGACAGACGACATCCCAAGCGGCGACGCAGTTGGTGAAACAGACGGTG
AGCAGACCATGACAGAGTCC

Downstream 100 bases:

>100_bases
TCGCGGCCCGTTGACGGCGCTGTGTCGGGGACCGGCAAGGGGTTGACCCATGTTGTCACTGTGCGAAAGGTCGCCCTAAG
ACCGCCCCGAGTCGGAATTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKLGDFDFDLPEELIATRPAVPRSSARLLVAEQDRISDGRVTDLLDWLAPGDRLVLNDTRVIPARLSGQRHRNSAQGPVS
ARIEVTLLEPQADGSWAGLLKPLKKIKIGETLRFSDALSAELVDVVDGQGRLRFNLAGDDFDAALAEAGAMPLPPYIAAK
RPADEQDKTDYQTVWARNSGAVAAPTASLHFDEPLLKALSEKGVTFTYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVTP
EAAAEIAATKAAGNRVIPVGTTALRLIESAARESGAITPWEGETDIFIYPGFEFRVADALMTNFHLPKSTLMMLVSALMG
QDRIRAIYAHAVKERYRFFSYGDASLLIP

Sequences:

>Translated_349_residues
MKLGDFDFDLPEELIATRPAVPRSSARLLVAEQDRISDGRVTDLLDWLAPGDRLVLNDTRVIPARLSGQRHRNSAQGPVS
ARIEVTLLEPQADGSWAGLLKPLKKIKIGETLRFSDALSAELVDVVDGQGRLRFNLAGDDFDAALAEAGAMPLPPYIAAK
RPADEQDKTDYQTVWARNSGAVAAPTASLHFDEPLLKALSEKGVTFTYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVTP
EAAAEIAATKAAGNRVIPVGTTALRLIESAARESGAITPWEGETDIFIYPGFEFRVADALMTNFHLPKSTLMMLVSALMG
QDRIRAIYAHAVKERYRFFSYGDASLLIP
>Mature_349_residues
MKLGDFDFDLPEELIATRPAVPRSSARLLVAEQDRISDGRVTDLLDWLAPGDRLVLNDTRVIPARLSGQRHRNSAQGPVS
ARIEVTLLEPQADGSWAGLLKPLKKIKIGETLRFSDALSAELVDVVDGQGRLRFNLAGDDFDAALAEAGAMPLPPYIAAK
RPADEQDKTDYQTVWARNSGAVAAPTASLHFDEPLLKALSEKGVTFTYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVTP
EAAAEIAATKAAGNRVIPVGTTALRLIESAARESGAITPWEGETDIFIYPGFEFRVADALMTNFHLPKSTLMMLVSALMG
QDRIRAIYAHAVKERYRFFSYGDASLLIP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=47.3087818696884, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SILST (Q1GHP0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613088.1
- ProteinModelPortal:   Q1GHP0
- SMR:   Q1GHP0
- STRING:   Q1GHP0
- GeneID:   4076326
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1093
- NMPDR:   fig|292414.1.peg.3772
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   KARIHEA
- PhylomeDB:   Q1GHP0
- ProtClustDB:   PRK00147
- BioCyc:   SSP292414:TM1040_1093-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38031; Mature: 38031

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGDFDFDLPEELIATRPAVPRSSARLLVAEQDRISDGRVTDLLDWLAPGDRLVLNDTR
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCE
VIPARLSGQRHRNSAQGPVSARIEVTLLEPQADGSWAGLLKPLKKIKIGETLRFSDALSA
EEEHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCHHHHH
ELVDVVDGQGRLRFNLAGDDFDAALAEAGAMPLPPYIAAKRPADEQDKTDYQTVWARNSG
HHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
AVAAPTASLHFDEPLLKALSEKGVTFTYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVTP
CEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCCCHHHHHHHHCCCCH
EAAAEIAATKAAGNRVIPVGTTALRLIESAARESGAITPWEGETDIFIYPGFEFRVADAL
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHH
MTNFHLPKSTLMMLVSALMGQDRIRAIYAHAVKERYRFFSYGDASLLIP
HHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCEEEECC
>Mature Secondary Structure
MKLGDFDFDLPEELIATRPAVPRSSARLLVAEQDRISDGRVTDLLDWLAPGDRLVLNDTR
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCE
VIPARLSGQRHRNSAQGPVSARIEVTLLEPQADGSWAGLLKPLKKIKIGETLRFSDALSA
EEEHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCHHHHH
ELVDVVDGQGRLRFNLAGDDFDAALAEAGAMPLPPYIAAKRPADEQDKTDYQTVWARNSG
HHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
AVAAPTASLHFDEPLLKALSEKGVTFTYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVTP
CEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCCCHHHHHHHHCCCCH
EAAAEIAATKAAGNRVIPVGTTALRLIESAARESGAITPWEGETDIFIYPGFEFRVADAL
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHH
MTNFHLPKSTLMMLVSALMGQDRIRAIYAHAVKERYRFFSYGDASLLIP
HHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA