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The map label for this gene is glnA [H]

Identifier: 99080863

GI number: 99080863

Start: 1093375

End: 1094781

Strand: Reverse

Name: glnA [H]

Synonym: TM1040_1022

Alternate gene names: 99080863

Gene position: 1094781-1093375 (Counterclockwise)

Preceding gene: 99080864

Following gene: 99080861

Centisome position: 34.2

GC content: 58.85

Gene sequence:

>1407_bases
ATGAGCAAAGATGCTGTTCTCAAGCTGATCAAGGATGAAGAGGCAGAATATGTCGACATCCGCTTCACCGACCCGCGCGG
CAAGCTCCAGCACGTCACCCTGATGTCGGATCAGGTCGACGAGGACTTCCTCGACGAGGGCTTCATGTTCGACGGCTCTT
CCATCGCAGGCTGGAAATCCATTGAAGCCTCCGACATGAAGCTGATCCCCGACACCGAATCCGCCTATGTCGACCCCTTC
TACGCCGAAAAAACCATCTGCATTCACTGCTCCGTGGTCGAGCCCGACACCGGCGAAGCCTATGAGCGCGACCCGCGCGG
CACTGCCGAAAAAGCAGAAGCCTACCTCAAGGCCTCCGGCATCGGTGACGTCGCCTACATGGGCCCCGAAGCAGAATTCT
TCCTGTTTGACGATGTACGTTTCTCCAACTCCATCAACAAAGTGTCCTACGAGGTCGATGCAACCGACGCCTCCTGGAAC
ACCGATGCTGAGTATGAAACCGGCAACATGGGCCACCGCCCGGGCGTCAAAGGCGGCTACTTCCCGGTAAACCCGGTCGA
CGAGTCCCAGGACCTGCGCTCCGAGATGCTCTCCACCATGAAGCGTCTGGGCATGAAAGTGGACAAGCACCACCACGAGG
TTGCGTCCTGTCAGCACGAGCTGGGTCTGATCTTTGACAGCCTGACCAAACAGGCCGACGAGCTGCAGAAGTACAAATAC
GTGATCCACAACGTGGCCCACGCCTATGGCAAATCCGCGACCTTCATGCCCAAGCCGATCGCAGGCGACAACGGCACCGG
CATGCACGTCAACATGTCCATCTGGAAAGACGGCAAGCCGCTCTTTGCAGGTGACAAATACGCGGACCTGTCGCAGGAAG
CGCTGTACTTCATCGGTGGTATCCTGAAGCACGCAAAAACCCTGAATGCTTTCACCAACCCGTCCACCAACTCCTACAAG
CGTCTGATCCCGGGCTTTGAAGCCCCCGTTCTGCGCGCGTATTCCGCTCGTAACCGTTCCGGCTGCGTGCGTATTCCGTG
GACCGAATCCCCGAAGGCAAAGCGCGTCGAGGCCCGCTTCCCCGATCCGGCGGCAAACCCCTACCTGGCGTTTGCAGCGC
TTCTGATGGCCGGCCTTGACGGCATCAAGAACAAGATCGATCCGGGCGAAGCCATGGATAAGAACCTCTATGACCTGCCC
GCAGAAGAGCTGGCCGACATCCCGACCGTCTGTGGCTCCCTGCGCGAAGCGCTCGAGTCGCTCGCGGCCGACCACGACTT
CCTGCTGCAGGGCGACGTGTTCACCAAAGACCAGATCGAAGGCTACATCGCCCTCAAGATGGAAGAGGTGGAAACCTACG
AGCACACCCCGCATCCGGTCGAATACCAGATGTACTACAGCTGCTGA

Upstream 100 bases:

>100_bases
TGACGCCCTTTAAGAGGTCTGCCCTGCGCGCGACCCCGTGCCGCCGGGCAACAATGAACACCCCGAAACACCCAAGTTTC
TCACGGAGGAATAACTTCAA

Downstream 100 bases:

>100_bases
TCTCTCGAGATTGGACTGATATGGAAAGGGCGCCCCATCTGGGCGCCCTTTTTCTATGCTCTGGCAGCCGGGGTTTTCAG
CTCGATCCTCGTCTCACTGA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSKDAVLKLIKDEEAEYVDIRFTDPRGKLQHVTLMSDQVDEDFLDEGFMFDGSSIAGWKSIEASDMKLIPDTESAYVDPF
YAEKTICIHCSVVEPDTGEAYERDPRGTAEKAEAYLKASGIGDVAYMGPEAEFFLFDDVRFSNSINKVSYEVDATDASWN
TDAEYETGNMGHRPGVKGGYFPVNPVDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFDSLTKQADELQKYKY
VIHNVAHAYGKSATFMPKPIAGDNGTGMHVNMSIWKDGKPLFAGDKYADLSQEALYFIGGILKHAKTLNAFTNPSTNSYK
RLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARFPDPAANPYLAFAALLMAGLDGIKNKIDPGEAMDKNLYDLP
AEELADIPTVCGSLREALESLAADHDFLLQGDVFTKDQIEGYIALKMEEVETYEHTPHPVEYQMYYSC

Sequences:

>Translated_468_residues
MSKDAVLKLIKDEEAEYVDIRFTDPRGKLQHVTLMSDQVDEDFLDEGFMFDGSSIAGWKSIEASDMKLIPDTESAYVDPF
YAEKTICIHCSVVEPDTGEAYERDPRGTAEKAEAYLKASGIGDVAYMGPEAEFFLFDDVRFSNSINKVSYEVDATDASWN
TDAEYETGNMGHRPGVKGGYFPVNPVDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFDSLTKQADELQKYKY
VIHNVAHAYGKSATFMPKPIAGDNGTGMHVNMSIWKDGKPLFAGDKYADLSQEALYFIGGILKHAKTLNAFTNPSTNSYK
RLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARFPDPAANPYLAFAALLMAGLDGIKNKIDPGEAMDKNLYDLP
AEELADIPTVCGSLREALESLAADHDFLLQGDVFTKDQIEGYIALKMEEVETYEHTPHPVEYQMYYSC
>Mature_467_residues
SKDAVLKLIKDEEAEYVDIRFTDPRGKLQHVTLMSDQVDEDFLDEGFMFDGSSIAGWKSIEASDMKLIPDTESAYVDPFY
AEKTICIHCSVVEPDTGEAYERDPRGTAEKAEAYLKASGIGDVAYMGPEAEFFLFDDVRFSNSINKVSYEVDATDASWNT
DAEYETGNMGHRPGVKGGYFPVNPVDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFDSLTKQADELQKYKYV
IHNVAHAYGKSATFMPKPIAGDNGTGMHVNMSIWKDGKPLFAGDKYADLSQEALYFIGGILKHAKTLNAFTNPSTNSYKR
LIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARFPDPAANPYLAFAALLMAGLDGIKNKIDPGEAMDKNLYDLPA
EELADIPTVCGSLREALESLAADHDFLLQGDVFTKDQIEGYIALKMEEVETYEHTPHPVEYQMYYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=59.9147121535181, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI87081870, Length=299, Percent_Identity=25.0836120401338, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52214; Mature: 52083

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDAVLKLIKDEEAEYVDIRFTDPRGKLQHVTLMSDQVDEDFLDEGFMFDGSSIAGWKS
CCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEHHHHCCHHHHHCCCEECCCCCCCCCC
IEASDMKLIPDTESAYVDPFYAEKTICIHCSVVEPDTGEAYERDPRGTAEKAEAYLKASG
CCCCCCEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHCC
IGDVAYMGPEAEFFLFDDVRFSNSINKVSYEVDATDASWNTDAEYETGNMGHRPGVKGGY
CCCEEEECCCCEEEEEECEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
FPVNPVDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFDSLTKQADELQKYKY
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIHNVAHAYGKSATFMPKPIAGDNGTGMHVNMSIWKDGKPLFAGDKYADLSQEALYFIGG
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
ILKHAKTLNAFTNPSTNSYKRLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARF
HHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEECCC
PDPAANPYLAFAALLMAGLDGIKNKIDPGEAMDKNLYDLPAEELADIPTVCGSLREALES
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAADHDFLLQGDVFTKDQIEGYIALKMEEVETYEHTPHPVEYQMYYSC
HHCCCCEEEECCCCCHHHCCEEEEEEEEHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
SKDAVLKLIKDEEAEYVDIRFTDPRGKLQHVTLMSDQVDEDFLDEGFMFDGSSIAGWKS
CHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEHHHHCCHHHHHCCCEECCCCCCCCCC
IEASDMKLIPDTESAYVDPFYAEKTICIHCSVVEPDTGEAYERDPRGTAEKAEAYLKASG
CCCCCCEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHCC
IGDVAYMGPEAEFFLFDDVRFSNSINKVSYEVDATDASWNTDAEYETGNMGHRPGVKGGY
CCCEEEECCCCEEEEEECEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
FPVNPVDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFDSLTKQADELQKYKY
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIHNVAHAYGKSATFMPKPIAGDNGTGMHVNMSIWKDGKPLFAGDKYADLSQEALYFIGG
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
ILKHAKTLNAFTNPSTNSYKRLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARF
HHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEECCC
PDPAANPYLAFAALLMAGLDGIKNKIDPGEAMDKNLYDLPAEELADIPTVCGSLREALES
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAADHDFLLQGDVFTKDQIEGYIALKMEEVETYEHTPHPVEYQMYYSC
HHCCCCEEEECCCCCHHHCCEEEEEEEEHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7921264 [H]