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The map label for this gene is mltB [H]

Identifier: 99080860

GI number: 99080860

Start: 1090440

End: 1091252

Strand: Reverse

Name: mltB [H]

Synonym: TM1040_1019

Alternate gene names: 99080860

Gene position: 1091252-1090440 (Counterclockwise)

Preceding gene: 99080861

Following gene: 99080859

Centisome position: 34.09

GC content: 62.12

Gene sequence:

>813_bases
ATGCTGTTTTCCCGTCTCGCCGCCCTTGGTCTTGTGACCTGCCTTGCCGTTGCCACCCCCGAGGTCGTGCGCGCCGCGAG
TTGTGGCAACACCGCCGCGGGGTTTGAAGCCTGGAAGGCCGATTTTGCGCGCACGGCCAAACGGGCCGGGGTCCGTCAGG
CCGGGCTCAACGCGCTGGCGCAAACCCGATATGCCACCGGCACCATCGCCGCCGACCGCAATCAGAAGAGCTTTCGCTAT
AGTCTTGAGAAGTTCATGCAGGTGCGCGGCGCGACAACCATCGTGGCACAGGGCCGCCGTCACAAGGCCCGCAATGCAGA
GTTCTTTGCCGCGCTTGAGCGCAAATATGGCGTGCCGGCGGGGCTGTTGATCGCCATTCATGGCATGGAGACCGCCTTTG
GCAATTATATGGGCGACAGTCAGGTGGTGTCGTCGATTGTGACCCTCACCTATGACTGCCGCCGCTCGGAGTTTTTTGAA
CCCCACGCCATCGGCGCGCTCAAGCTCGTGGATCAGGGTGCGATCTCGATGGCCACCAAAGGTGCGAAACACGGGGAGCT
GGGCCACACGCAGTTTCTGCCAGGCAACGCACTGAAATATGGTCAGGACTGGAACCGCGACGGGCGGGTGGATTTCTACG
ACATGGGCGACGCACTCGCCTCTACCGCGCATTTCCTGCGTCAAAAAGGCTGGCGGCCGGGCAAAGGCTATCAGCAGGGC
GAGCCCAACTACAAAGTGCTCAAACAATGGAATGCCGCCACCGTGTACCAGCAGTCCCTCGCCATCATGGGCCGGCAGAT
CGACGGCTCCTGA

Upstream 100 bases:

>100_bases
TGGCGCCGCCTGACAAAAGCTCCCCGCAAATAAGAGATGAAAACAGAGGCTCCGGCGCGTAGGTTGATCCTATCCGCGCC
ATTTCCAATGAGGGTCTTTC

Downstream 100 bases:

>100_bases
GCCTGAGATCCGGGCGTTAAGAACAATCAACGACGAGGTCGCCAGATTGTAAGGGCTTGGGCATCAATGTGGCTGTCAGA
GGTCCAGTTCCTGCCGCATT

Product: hypothetical protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLFSRLAALGLVTCLAVATPEVVRAASCGNTAAGFEAWKADFARTAKRAGVRQAGLNALAQTRYATGTIAADRNQKSFRY
SLEKFMQVRGATTIVAQGRRHKARNAEFFAALERKYGVPAGLLIAIHGMETAFGNYMGDSQVVSSIVTLTYDCRRSEFFE
PHAIGALKLVDQGAISMATKGAKHGELGHTQFLPGNALKYGQDWNRDGRVDFYDMGDALASTAHFLRQKGWRPGKGYQQG
EPNYKVLKQWNAATVYQQSLAIMGRQIDGS

Sequences:

>Translated_270_residues
MLFSRLAALGLVTCLAVATPEVVRAASCGNTAAGFEAWKADFARTAKRAGVRQAGLNALAQTRYATGTIAADRNQKSFRY
SLEKFMQVRGATTIVAQGRRHKARNAEFFAALERKYGVPAGLLIAIHGMETAFGNYMGDSQVVSSIVTLTYDCRRSEFFE
PHAIGALKLVDQGAISMATKGAKHGELGHTQFLPGNALKYGQDWNRDGRVDFYDMGDALASTAHFLRQKGWRPGKGYQQG
EPNYKVLKQWNAATVYQQSLAIMGRQIDGS
>Mature_270_residues
MLFSRLAALGLVTCLAVATPEVVRAASCGNTAAGFEAWKADFARTAKRAGVRQAGLNALAQTRYATGTIAADRNQKSFRY
SLEKFMQVRGATTIVAQGRRHKARNAEFFAALERKYGVPAGLLIAIHGMETAFGNYMGDSQVVSSIVTLTYDCRRSEFFE
PHAIGALKLVDQGAISMATKGAKHGELGHTQFLPGNALKYGQDWNRDGRVDFYDMGDALASTAHFLRQKGWRPGKGYQQG
EPNYKVLKQWNAATVYQQSLAIMGRQIDGS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=121, Percent_Identity=31.404958677686, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 29479; Mature: 29479

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSRLAALGLVTCLAVATPEVVRAASCGNTAAGFEAWKADFARTAKRAGVRQAGLNALA
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
QTRYATGTIAADRNQKSFRYSLEKFMQVRGATTIVAQGRRHKARNAEFFAALERKYGVPA
HHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHEECCCCCCCCCCHHHHHHHHHHCCCCC
GLLIAIHGMETAFGNYMGDSQVVSSIVTLTYDCRRSEFFEPHAIGALKLVDQGAISMATK
EEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHC
GAKHGELGHTQFLPGNALKYGQDWNRDGRVDFYDMGDALASTAHFLRQKGWRPGKGYQQG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
EPNYKVLKQWNAATVYQQSLAIMGRQIDGS
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLFSRLAALGLVTCLAVATPEVVRAASCGNTAAGFEAWKADFARTAKRAGVRQAGLNALA
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
QTRYATGTIAADRNQKSFRYSLEKFMQVRGATTIVAQGRRHKARNAEFFAALERKYGVPA
HHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHEECCCCCCCCCCHHHHHHHHHHCCCCC
GLLIAIHGMETAFGNYMGDSQVVSSIVTLTYDCRRSEFFEPHAIGALKLVDQGAISMATK
EEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHC
GAKHGELGHTQFLPGNALKYGQDWNRDGRVDFYDMGDALASTAHFLRQKGWRPGKGYQQG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
EPNYKVLKQWNAATVYQQSLAIMGRQIDGS
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]