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The map label for this gene is dapE [H]

Identifier: 99080700

GI number: 99080700

Start: 911391

End: 912782

Strand: Reverse

Name: dapE [H]

Synonym: TM1040_0859

Alternate gene names: 99080700

Gene position: 912782-911391 (Counterclockwise)

Preceding gene: 99080704

Following gene: 99080698

Centisome position: 28.52

GC content: 61.14

Gene sequence:

>1392_bases
ATGTCCCTTACCCCTGTTCTCGACAAAATCGATTCCGATGTTTCCGCCGCCACCGACCGGCTGCTGGAACTGTTGCGCAT
TCCCTCCATTTCCACTGATCCCGCCTACAAGGCCGACTGCGACGCCGCTGCGGATTGGTTGGTCAAGGATCTGAACTCCA
TCGGCATCAAGGCTGAGAAGCGCGAAACACCCGGCCATCCGATGGTGGTGGGCCATGTGGGCGAGGAAAACACCAAAGGC
CCGCATCTGCTGTTTTACGGCCATTATGATGTGCAGCCCGTCGATCCGCTGAACCTCTGGAACACGCCACCCTTTGAGCC
TCAGCTTGAAGAAACGCCCAATGGCACGGTGATCCGCGGCCGCGGTGCATCGGATGACAAGGGTCAGTTGATGACCTTTG
TAGAAGCCTGCCGGGCGTGGCAGGCGGTGCATGGCACCCTGCCCTGCCGCATCACCTTTTTCTTCGAGGGCGAAGAGGAA
TCAGGGTCGCCCTCGCTCATTCCGTTCCTCGAGGCCGCCAAAGAAGAGCTCTCTGCCGATATCGCGCTGATCTGCGACAC
GTCTATGGTGGCGCGCGGGGTGCCTTCGGTGGCGTCGCAGCTGCGCGGCATGATGAAGGATGAATTCACCCTGATCGGCC
CCAAGATCGACCTGCATTCGGGGCACTATGGTGGCCCTGGTCTGAACCCGCTGCGCGAGATCTCCCACATCATTGCATCC
TTCCATGACCCCGACACCGGCGCTGTGGCCGTGGAGGGCTTTTACGAGGGCGTGCCGGAGGTGCCGGCGGATATCCTGCG
ACAGTGGGAAGGCTGTGGCTTTGACGAGAAGGACTATCTCTCCAACGCGGGCTACACCGAGGCGCATGGCGAAAAGAAAT
ACTCCACCCTGGAGCAGCAATGGGCGCGTCCGACCCTCGAGATCAACGGGCTTTGGGGCGGCTATCAGGGCGCAGGCACC
AAGACGGTGATTCCCTCAGAGGCGCATTGCAAGATCACCTGCCGCCTGGTGGGCGACATGGACCCTGCCGATCTGCGCCA
GAAGATTCGCAAACATGTCGAAGACCGCCTGCCTACGGATTGCACCGTGAAATGGGACCTCGATCTGGATGGCGCACCCG
CCTATGTGATCGACACCACCCGGCCCGAGTTCGAGGCCGCGCGCGGCGCTTTGACCGAGGAATGGAACCGCGAGGCGGTG
TTCTCCGGCATGGGCGGGTCGATTCCGGTGGCGGGCTATTTCAAATCGATCCTCGGCATGGACGCGATGCTGATTGGCTT
TGCCAATGAGGATGACGCGATCCACTCCCCCAATGAGAAATACGACCTCGAGAGCTTCCACAAGGGGATCCGCTCTTGGG
CGCGGGTGCTGGACGCCCTGAGCAAAGCCTGA

Upstream 100 bases:

>100_bases
CTTGTCATGCAGCGCTGTTAGGCTCCGCCACGGACCCGCCCTGCCCTGTTGAAGCTCATCACGGCCCAAGGGCAACACGG
CACTATTTCAGGAGAGACCC

Downstream 100 bases:

>100_bases
GGGGCTCAGGGCGCAGTGGCGCCCTGCCCTCTCCCCGAAACGATCAGACCAGCCCTCGCAGCCTCTGCGGGGGCTGTGTC
GTTTTACGCCTCGCGCAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSLTPVLDKIDSDVSAATDRLLELLRIPSISTDPAYKADCDAAADWLVKDLNSIGIKAEKRETPGHPMVVGHVGEENTKG
PHLLFYGHYDVQPVDPLNLWNTPPFEPQLEETPNGTVIRGRGASDDKGQLMTFVEACRAWQAVHGTLPCRITFFFEGEEE
SGSPSLIPFLEAAKEELSADIALICDTSMVARGVPSVASQLRGMMKDEFTLIGPKIDLHSGHYGGPGLNPLREISHIIAS
FHDPDTGAVAVEGFYEGVPEVPADILRQWEGCGFDEKDYLSNAGYTEAHGEKKYSTLEQQWARPTLEINGLWGGYQGAGT
KTVIPSEAHCKITCRLVGDMDPADLRQKIRKHVEDRLPTDCTVKWDLDLDGAPAYVIDTTRPEFEAARGALTEEWNREAV
FSGMGGSIPVAGYFKSILGMDAMLIGFANEDDAIHSPNEKYDLESFHKGIRSWARVLDALSKA

Sequences:

>Translated_463_residues
MSLTPVLDKIDSDVSAATDRLLELLRIPSISTDPAYKADCDAAADWLVKDLNSIGIKAEKRETPGHPMVVGHVGEENTKG
PHLLFYGHYDVQPVDPLNLWNTPPFEPQLEETPNGTVIRGRGASDDKGQLMTFVEACRAWQAVHGTLPCRITFFFEGEEE
SGSPSLIPFLEAAKEELSADIALICDTSMVARGVPSVASQLRGMMKDEFTLIGPKIDLHSGHYGGPGLNPLREISHIIAS
FHDPDTGAVAVEGFYEGVPEVPADILRQWEGCGFDEKDYLSNAGYTEAHGEKKYSTLEQQWARPTLEINGLWGGYQGAGT
KTVIPSEAHCKITCRLVGDMDPADLRQKIRKHVEDRLPTDCTVKWDLDLDGAPAYVIDTTRPEFEAARGALTEEWNREAV
FSGMGGSIPVAGYFKSILGMDAMLIGFANEDDAIHSPNEKYDLESFHKGIRSWARVLDALSKA
>Mature_462_residues
SLTPVLDKIDSDVSAATDRLLELLRIPSISTDPAYKADCDAAADWLVKDLNSIGIKAEKRETPGHPMVVGHVGEENTKGP
HLLFYGHYDVQPVDPLNLWNTPPFEPQLEETPNGTVIRGRGASDDKGQLMTFVEACRAWQAVHGTLPCRITFFFEGEEES
GSPSLIPFLEAAKEELSADIALICDTSMVARGVPSVASQLRGMMKDEFTLIGPKIDLHSGHYGGPGLNPLREISHIIASF
HDPDTGAVAVEGFYEGVPEVPADILRQWEGCGFDEKDYLSNAGYTEAHGEKKYSTLEQQWARPTLEINGLWGGYQGAGTK
TVIPSEAHCKITCRLVGDMDPADLRQKIRKHVEDRLPTDCTVKWDLDLDGAPAYVIDTTRPEFEAARGALTEEWNREAVF
SGMGGSIPVAGYFKSILGMDAMLIGFANEDDAIHSPNEKYDLESFHKGIRSWARVLDALSKA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=479, Percent_Identity=27.348643006263, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI21071039, Length=401, Percent_Identity=28.927680798005, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI271398277, Length=323, Percent_Identity=28.1733746130031, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788816, Length=223, Percent_Identity=29.1479820627803, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17563146, Length=477, Percent_Identity=29.979035639413, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI193211059, Length=480, Percent_Identity=28.75, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321159, Length=482, Percent_Identity=29.253112033195, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319758, Length=414, Percent_Identity=25.1207729468599, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24585880, Length=468, Percent_Identity=29.2735042735043, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50770; Mature: 50638

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTPVLDKIDSDVSAATDRLLELLRIPSISTDPAYKADCDAAADWLVKDLNSIGIKAEK
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEC
RETPGHPMVVGHVGEENTKGPHLLFYGHYDVQPVDPLNLWNTPPFEPQLEETPNGTVIRG
CCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
RGASDDKGQLMTFVEACRAWQAVHGTLPCRITFFFEGEEESGSPSLIPFLEAAKEELSAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
IALICDTSMVARGVPSVASQLRGMMKDEFTLIGPKIDLHSGHYGGPGLNPLREISHIIAS
EEEEECCHHHHCCCHHHHHHHHHHHHCCEEEECCEEEECCCCCCCCCCCHHHHHHHHHHH
FHDPDTGAVAVEGFYEGVPEVPADILRQWEGCGFDEKDYLSNAGYTEAHGEKKYSTLEQQ
CCCCCCCEEEECHHHCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCHHHHHHHHH
WARPTLEINGLWGGYQGAGTKTVIPSEAHCKITCRLVGDMDPADLRQKIRKHVEDRLPTD
HCCCCEEEECCCCCCCCCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
CTVKWDLDLDGAPAYVIDTTRPEFEAARGALTEEWNREAVFSGMGGSIPVAGYFKSILGM
CEEEEECCCCCCCEEEEECCCCHHHHHHCCHHHHCCCHHHHCCCCCCCCHHHHHHHHHCC
DAMLIGFANEDDAIHSPNEKYDLESFHKGIRSWARVLDALSKA
CEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLTPVLDKIDSDVSAATDRLLELLRIPSISTDPAYKADCDAAADWLVKDLNSIGIKAEK
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEC
RETPGHPMVVGHVGEENTKGPHLLFYGHYDVQPVDPLNLWNTPPFEPQLEETPNGTVIRG
CCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
RGASDDKGQLMTFVEACRAWQAVHGTLPCRITFFFEGEEESGSPSLIPFLEAAKEELSAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
IALICDTSMVARGVPSVASQLRGMMKDEFTLIGPKIDLHSGHYGGPGLNPLREISHIIAS
EEEEECCHHHHCCCHHHHHHHHHHHHCCEEEECCEEEECCCCCCCCCCCHHHHHHHHHHH
FHDPDTGAVAVEGFYEGVPEVPADILRQWEGCGFDEKDYLSNAGYTEAHGEKKYSTLEQQ
CCCCCCCEEEECHHHCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCHHHHHHHHH
WARPTLEINGLWGGYQGAGTKTVIPSEAHCKITCRLVGDMDPADLRQKIRKHVEDRLPTD
HCCCCEEEECCCCCCCCCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
CTVKWDLDLDGAPAYVIDTTRPEFEAARGALTEEWNREAVFSGMGGSIPVAGYFKSILGM
CEEEEECCCCCCCEEEEECCCCHHHHHHCCHHHHCCCHHHHCCCCCCCCHHHHHHHHHCC
DAMLIGFANEDDAIHSPNEKYDLESFHKGIRSWARVLDALSKA
CEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]