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The map label for this gene is pleD [H]

Identifier: 94985714

GI number: 94985714

Start: 1716136

End: 1717731

Strand: Reverse

Name: pleD [H]

Synonym: Dgeo_1614

Alternate gene names: 94985714

Gene position: 1717731-1716136 (Counterclockwise)

Preceding gene: 94985715

Following gene: 94985713

Centisome position: 69.62

GC content: 67.98

Gene sequence:

>1596_bases
ATGAACCTTGCCTACGCGGCCTGGCTCCTGGCGGCGACGCTGATGGCTTTGTTGACGGCGGGGGTGGCCTGGCATCGCCG
CAGCGCGCCGGGCGCCTGGCCGCTGGTGCTGCTGCTGCTCGCGCTGGCCGAGTGGACCTTCACCTATGCCCTGCACTGGC
TGGTGGCGGCGCCCGAGGAGCGGCGCGGATGGCTGGACGCTACCTTTGTGGGCGTTTCTCTGGCACCGGTCTTCGTGCTT
TTGATGACGCTGGAACTCACCGAGCGGTCCTTGAACCGCGCGGCCAGGACGGCCCTGTTCGGCGTGCCGCTGGTCACCCT
GGTGCTGCTGCTGGCCGGGGATCCCCAGGGGCTGCTGTTCGGGGTGCGGCCACTCGACGCGCACAATCTGTTGCAGGGTG
GTCCCTGGTTCCGGGTGGTGGCCCTGTATGGCTACGCGCTGCTGGTGCTCAGTGCGGCCCTGCTGATCCACTTTTCCTGG
CGCTCGCCGCCCCTCTACCGGCGGCAGGCAACGCTGCTGCTGGCCGGGCTGGTGTTGCCCTGGGGCGTCAATGTGGTGAG
TGTGCTGGGGGCGCGGCCCTTTCCGGACCTCGACCTGACGCCGGTGCTGTTCCTGCTGACTGCGCTGGTGTTCCTGTGGG
GCATTGTGGGCCTCAAGCTGTTTGACGTGCTGCCGGTGGCCCGGCACCTGTTGGTCGAGCAGATGGCGGAAGGTGTGATG
GTGCTTGATGAGCGAGGTCGGGTGGTCGACCTCAATCCGGCGGCGCGCAGGCTCAGCCGCTGGTCTGGCCCCTCCCCGCT
TGGTCAGTCTGTAGACGTGGTCTTTGCCAATTGGAGTCACGTGTTGGAGCGCTACCGGGAGGTTGAGCAGGCCCATGCCG
AGCTGTTGCTGGATGGAAAGGTGTTCCTGGACCTGCAAATCTCGCCGCTGTATGACGGACGGGGGCGACTGCGGGGGCGA
CTGGTGGTATGGCGCGACATGACCGCGCGCCGTGAGGCCGAGACGCGGCTGCGACAGGCCCACGCGGAGCTGCAGGCCCG
GCTGAGCGAGATTGAGCAGCTGCAACTGGCCCTCCAGGAACAGAGCCTGCGCGATCCCCTCACCGGCCTGCACAATCGCC
GTTTCCTCCAAACAGCCCTGGAAGAGGCGCAGGGTCGGGCAGCGGCGAGTGCGCAGCCCTTTAGCCTGGTGATTGTGGAT
ATCGACCACTTCAAGCAGATCAACGATCGGGTGGGACACGGGGGCGGGGACGCCGTCTTACACAGCGTCGCGCGCCTGCT
TGAAGCCCACCGGGGTACAGGGGAGACGCTCTGCCGCTACGGAGGGGAAGAATTCGTGCTGGTTCTGCCCGATACGCCGC
TGGAAGCGGCCCTCACCCGGGCCGAAGCCTACCGGGCCGCTCTGGAGGGCCAGGTGGTGATGTACGGCGGTCAGGCGCTG
CGTTGCACGGCCTCGCTGGGGGTGGCCACCTTTCCCACACACGGGACTGCTGCCGAGGTGGTGCTGACGGCGGCGGACGA
GGCCCTCTACACGGCCAAGCGCCAGGGGCGCAACCGGGTGGTGGCCGCGCCCCCGGCAGCAGCACCGCTATCCTGA

Upstream 100 bases:

>100_bases
CCGGTACCCTGACGGCAAGCATGACGCTGCTGCTCCACAGCCCAGGTGGCTGCGTTCTCCTGTCTCGCCACAACATTCCA
GGGGTGAGAGGGGAGTCCAG

Downstream 100 bases:

>100_bases
GACGGCATGACTGCGCCAACCCTCGAAACCCGCTTTCTGGCCGGTGATCCCCGCGCCCTGGCCCGCGCGATCACGCTGGC
CGAGAGCGGCCTGCCCGCTG

Product: diguanylate cyclase with PAS/PAC sensor

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MNLAYAAWLLAATLMALLTAGVAWHRRSAPGAWPLVLLLLALAEWTFTYALHWLVAAPEERRGWLDATFVGVSLAPVFVL
LMTLELTERSLNRAARTALFGVPLVTLVLLLAGDPQGLLFGVRPLDAHNLLQGGPWFRVVALYGYALLVLSAALLIHFSW
RSPPLYRRQATLLLAGLVLPWGVNVVSVLGARPFPDLDLTPVLFLLTALVFLWGIVGLKLFDVLPVARHLLVEQMAEGVM
VLDERGRVVDLNPAARRLSRWSGPSPLGQSVDVVFANWSHVLERYREVEQAHAELLLDGKVFLDLQISPLYDGRGRLRGR
LVVWRDMTARREAETRLRQAHAELQARLSEIEQLQLALQEQSLRDPLTGLHNRRFLQTALEEAQGRAAASAQPFSLVIVD
IDHFKQINDRVGHGGGDAVLHSVARLLEAHRGTGETLCRYGGEEFVLVLPDTPLEAALTRAEAYRAALEGQVVMYGGQAL
RCTASLGVATFPTHGTAAEVVLTAADEALYTAKRQGRNRVVAAPPAAAPLS

Sequences:

>Translated_531_residues
MNLAYAAWLLAATLMALLTAGVAWHRRSAPGAWPLVLLLLALAEWTFTYALHWLVAAPEERRGWLDATFVGVSLAPVFVL
LMTLELTERSLNRAARTALFGVPLVTLVLLLAGDPQGLLFGVRPLDAHNLLQGGPWFRVVALYGYALLVLSAALLIHFSW
RSPPLYRRQATLLLAGLVLPWGVNVVSVLGARPFPDLDLTPVLFLLTALVFLWGIVGLKLFDVLPVARHLLVEQMAEGVM
VLDERGRVVDLNPAARRLSRWSGPSPLGQSVDVVFANWSHVLERYREVEQAHAELLLDGKVFLDLQISPLYDGRGRLRGR
LVVWRDMTARREAETRLRQAHAELQARLSEIEQLQLALQEQSLRDPLTGLHNRRFLQTALEEAQGRAAASAQPFSLVIVD
IDHFKQINDRVGHGGGDAVLHSVARLLEAHRGTGETLCRYGGEEFVLVLPDTPLEAALTRAEAYRAALEGQVVMYGGQAL
RCTASLGVATFPTHGTAAEVVLTAADEALYTAKRQGRNRVVAAPPAAAPLS
>Mature_531_residues
MNLAYAAWLLAATLMALLTAGVAWHRRSAPGAWPLVLLLLALAEWTFTYALHWLVAAPEERRGWLDATFVGVSLAPVFVL
LMTLELTERSLNRAARTALFGVPLVTLVLLLAGDPQGLLFGVRPLDAHNLLQGGPWFRVVALYGYALLVLSAALLIHFSW
RSPPLYRRQATLLLAGLVLPWGVNVVSVLGARPFPDLDLTPVLFLLTALVFLWGIVGLKLFDVLPVARHLLVEQMAEGVM
VLDERGRVVDLNPAARRLSRWSGPSPLGQSVDVVFANWSHVLERYREVEQAHAELLLDGKVFLDLQISPLYDGRGRLRGR
LVVWRDMTARREAETRLRQAHAELQARLSEIEQLQLALQEQSLRDPLTGLHNRRFLQTALEEAQGRAAASAQPFSLVIVD
IDHFKQINDRVGHGGGDAVLHSVARLLEAHRGTGETLCRYGGEEFVLVLPDTPLEAALTRAEAYRAALEGQVVMYGGQAL
RCTASLGVATFPTHGTAAEVVLTAADEALYTAKRQGRNRVVAAPPAAAPLS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=42.6136363636364, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788381, Length=312, Percent_Identity=26.9230769230769, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=38.8535031847134, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=181, Percent_Identity=38.6740331491713, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=32.5714285714286, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=35.8024691358025, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=33.1360946745562, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788956, Length=204, Percent_Identity=31.3725490196078, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=33.5365853658537, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081977, Length=182, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787541, Length=309, Percent_Identity=25.8899676375405, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=31.638418079096, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=26.4150943396226, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788549, Length=185, Percent_Identity=28.1081081081081, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 58208; Mature: 58208

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAYAAWLLAATLMALLTAGVAWHRRSAPGAWPLVLLLLALAEWTFTYALHWLVAAPEE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RRGWLDATFVGVSLAPVFVLLMTLELTERSLNRAARTALFGVPLVTLVLLLAGDPQGLLF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
GVRPLDAHNLLQGGPWFRVVALYGYALLVLSAALLIHFSWRSPPLYRRQATLLLAGLVLP
ECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHH
WGVNVVSVLGARPFPDLDLTPVLFLLTALVFLWGIVGLKLFDVLPVARHLLVEQMAEGVM
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VLDERGRVVDLNPAARRLSRWSGPSPLGQSVDVVFANWSHVLERYREVEQAHAELLLDGK
EEECCCCEEECCHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHEEECCE
VFLDLQISPLYDGRGRLRGRLVVWRDMTARREAETRLRQAHAELQARLSEIEQLQLALQE
EEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSLRDPLTGLHNRRFLQTALEEAQGRAAASAQPFSLVIVDIDHFKQINDRVGHGGGDAVL
HHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHH
HSVARLLEAHRGTGETLCRYGGEEFVLVLPDTPLEAALTRAEAYRAALEGQVVMYGGQAL
HHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCEE
RCTASLGVATFPTHGTAAEVVLTAADEALYTAKRQGRNRVVAAPPAAAPLS
EEEECCCCEECCCCCCHHHEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MNLAYAAWLLAATLMALLTAGVAWHRRSAPGAWPLVLLLLALAEWTFTYALHWLVAAPEE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RRGWLDATFVGVSLAPVFVLLMTLELTERSLNRAARTALFGVPLVTLVLLLAGDPQGLLF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
GVRPLDAHNLLQGGPWFRVVALYGYALLVLSAALLIHFSWRSPPLYRRQATLLLAGLVLP
ECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHH
WGVNVVSVLGARPFPDLDLTPVLFLLTALVFLWGIVGLKLFDVLPVARHLLVEQMAEGVM
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VLDERGRVVDLNPAARRLSRWSGPSPLGQSVDVVFANWSHVLERYREVEQAHAELLLDGK
EEECCCCEEECCHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHEEECCE
VFLDLQISPLYDGRGRLRGRLVVWRDMTARREAETRLRQAHAELQARLSEIEQLQLALQE
EEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSLRDPLTGLHNRRFLQTALEEAQGRAAASAQPFSLVIVDIDHFKQINDRVGHGGGDAVL
HHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHH
HSVARLLEAHRGTGETLCRYGGEEFVLVLPDTPLEAALTRAEAYRAALEGQVVMYGGQAL
HHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCEE
RCTASLGVATFPTHGTAAEVVLTAADEALYTAKRQGRNRVVAAPPAAAPLS
EEEECCCCEECCCCCCHHHEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA