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The map label for this gene is ltaA [H]

Identifier: 94985551

GI number: 94985551

Start: 1538020

End: 1539057

Strand: Reverse

Name: ltaA [H]

Synonym: Dgeo_1451

Alternate gene names: 94985551

Gene position: 1539057-1538020 (Counterclockwise)

Preceding gene: 94985552

Following gene: 94985550

Centisome position: 62.38

GC content: 70.04

Gene sequence:

>1038_bases
ATGACCGCCACCCTGCCCGCGACGACCCGGCCCCACGTGATCGCCGACCTGCGCTCCGACACCGTGACCACGCCGACGCC
CGAGATGCGCGAGGCGATGGCACAGGCTCCGGTCGGGGATGACGTGTACGGCGAGGATCCCACCGTCAATGCCCTGCAGG
CGGAAGTCGCGCGCTTGACCGGACATGAGGCGGGCCTCTTTATGCCCAGCGGCACGATGACCAACCAGGTGGCGATCGCC
CTGCACACCCGCCGCGGCGAGGAGGTCATTTGCGCCGAGGGATCGCACATCTATGAGTGGGAACTTGGGATGATGGCGAC
CTTTTCCGGCGTGGTGCCGCGCTTCGTGCCCGCCCCGCTGGGGGTGCCGGACCCCGAAGCAGTGCGTTTGGCGGTGCGGC
GCTCGGTCCACCAGTCGCCCACCGGGCTGATCAGCCTCGAGAACACCCACAACAAGGCGGGCGGTACAGTGATTCCGCTG
GACGTGCTGGCCGCCATTCGTCATGTCGCGGACGACGAGGGCCTGCCGCTGCACCTCGACGGGGCGCGGGTGTTCAACGC
AGCAGCGGCCCTGGACGTGCCCGTCTCGGAGATCACCCGGCAGTTTGACACGGTGAGTGTCTGCCTCAGCAAGGGGCTGG
GGGCGCCGGTCGGGAGCGTGCTCGTGGGCAGTGCCGCCGCCATGCAGCAGGCGCACCGCTACCGCAAGATGATGGGCGGT
GGGATGCGGCAGGCCGGGGTGCTGGCTGCCGCCGCGCTGATCGCTCTGCGGGATGGTCCCGCCCGGCTGAAGGAGGACCA
CCGCCGCGCCCGGATTCTGGCCGAGGCGCTGGCTGAAGCGGGGTTTGACGTGGACCTCGCCGCCGTGCAGACGAACATGG
TCTATGTGACCCTGCCGGACGCGGCGGCGCAGGTGGCGCGCTGGGCTTCGCTGGGCGTGCTGGCGAGCGCACTTGGCCCG
GACTCGGTGCGCTTCGTGCTGCACCACCAGATCAGTGACGCGGCGCTGGCAGAGGCCCTCCACGTGCTGACGGCATGA

Upstream 100 bases:

>100_bases
AAGCAGCTCAGGATCTATCTCCGCGACTGACTGCGCCCTGGTGGGCCGTGGGGAAGAGGTTTCTCCGTTGGGTCGTGGGA
TGGCAGAGTAGCATGCCCCC

Downstream 100 bases:

>100_bases
AGGGCCAAGCCCTGTCTGGGTCGCGGGAAAGGGTGAAGTGTCTCCGCCTTTCGGTGGAGGTGTTCCTCGCCTGCGCCGCG
CTAGGCTGAGGGGTATGACG

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTATLPATTRPHVIADLRSDTVTTPTPEMREAMAQAPVGDDVYGEDPTVNALQAEVARLTGHEAGLFMPSGTMTNQVAIA
LHTRRGEEVICAEGSHIYEWELGMMATFSGVVPRFVPAPLGVPDPEAVRLAVRRSVHQSPTGLISLENTHNKAGGTVIPL
DVLAAIRHVADDEGLPLHLDGARVFNAAAALDVPVSEITRQFDTVSVCLSKGLGAPVGSVLVGSAAAMQQAHRYRKMMGG
GMRQAGVLAAAALIALRDGPARLKEDHRRARILAEALAEAGFDVDLAAVQTNMVYVTLPDAAAQVARWASLGVLASALGP
DSVRFVLHHQISDAALAEALHVLTA

Sequences:

>Translated_345_residues
MTATLPATTRPHVIADLRSDTVTTPTPEMREAMAQAPVGDDVYGEDPTVNALQAEVARLTGHEAGLFMPSGTMTNQVAIA
LHTRRGEEVICAEGSHIYEWELGMMATFSGVVPRFVPAPLGVPDPEAVRLAVRRSVHQSPTGLISLENTHNKAGGTVIPL
DVLAAIRHVADDEGLPLHLDGARVFNAAAALDVPVSEITRQFDTVSVCLSKGLGAPVGSVLVGSAAAMQQAHRYRKMMGG
GMRQAGVLAAAALIALRDGPARLKEDHRRARILAEALAEAGFDVDLAAVQTNMVYVTLPDAAAQVARWASLGVLASALGP
DSVRFVLHHQISDAALAEALHVLTA
>Mature_344_residues
TATLPATTRPHVIADLRSDTVTTPTPEMREAMAQAPVGDDVYGEDPTVNALQAEVARLTGHEAGLFMPSGTMTNQVAIAL
HTRRGEEVICAEGSHIYEWELGMMATFSGVVPRFVPAPLGVPDPEAVRLAVRRSVHQSPTGLISLENTHNKAGGTVIPLD
VLAAIRHVADDEGLPLHLDGARVFNAAAALDVPVSEITRQFDTVSVCLSKGLGAPVGSVLVGSAAAMQQAHRYRKMMGGG
MRQAGVLAAAALIALRDGPARLKEDHRRARILAEALAEAGFDVDLAAVQTNMVYVTLPDAAAQVARWASLGVLASALGPD
SVRFVLHHQISDAALAEALHVLTA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=327, Percent_Identity=46.177370030581, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI193206522, Length=352, Percent_Identity=41.7613636363636, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI193206520, Length=352, Percent_Identity=41.7613636363636, Blast_Score=247, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI193206524, Length=352, Percent_Identity=41.7613636363636, Blast_Score=247, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6320789, Length=293, Percent_Identity=42.6621160409556, Blast_Score=203, Evalue=3e-53,
Organism=Drosophila melanogaster, GI21355439, Length=366, Percent_Identity=44.8087431693989, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36273; Mature: 36142

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLPATTRPHVIADLRSDTVTTPTPEMREAMAQAPVGDDVYGEDPTVNALQAEVARLT
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
GHEAGLFMPSGTMTNQVAIALHTRRGEEVICAEGSHIYEWELGMMATFSGVVPRFVPAPL
CCCCCEEECCCCCCCEEEEEEEECCCCEEEEECCCEEEEEECCHHHHHHHCCHHHCCCCC
GVPDPEAVRLAVRRSVHQSPTGLISLENTHNKAGGTVIPLDVLAAIRHVADDEGLPLHLD
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCEEEEC
GARVFNAAAALDVPVSEITRQFDTVSVCLSKGLGAPVGSVLVGSAAAMQQAHRYRKMMGG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GMRQAGVLAAAALIALRDGPARLKEDHRRARILAEALAEAGFDVDLAAVQTNMVYVTLPD
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCH
AAAQVARWASLGVLASALGPDSVRFVLHHQISDAALAEALHVLTA
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATLPATTRPHVIADLRSDTVTTPTPEMREAMAQAPVGDDVYGEDPTVNALQAEVARLT
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
GHEAGLFMPSGTMTNQVAIALHTRRGEEVICAEGSHIYEWELGMMATFSGVVPRFVPAPL
CCCCCEEECCCCCCCEEEEEEEECCCCEEEEECCCEEEEEECCHHHHHHHCCHHHCCCCC
GVPDPEAVRLAVRRSVHQSPTGLISLENTHNKAGGTVIPLDVLAAIRHVADDEGLPLHLD
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCEEEEC
GARVFNAAAALDVPVSEITRQFDTVSVCLSKGLGAPVGSVLVGSAAAMQQAHRYRKMMGG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GMRQAGVLAAAALIALRDGPARLKEDHRRARILAEALAEAGFDVDLAAVQTNMVYVTLPD
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCH
AAAQVARWASLGVLASALGPDSVRFVLHHQISDAALAEALHVLTA
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]