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The map label for this gene is gutB [H]

Identifier: 94972327

GI number: 94972327

Start: 158030

End: 159109

Strand: Direct

Name: gutB [H]

Synonym: Dgeo_2864

Alternate gene names: 94972327

Gene position: 158030-159109 (Clockwise)

Preceding gene: 94972328

Following gene: 94972326

Centisome position: 27.53

GC content: 67.41

Gene sequence:

>1080_bases
ATGACTTCCCCGGATTCCTCTTTGTCTTCCCGCATCAGCGTTTTGCATGGCATCCGCGACCTGCGCTGGGAGACGCGCGA
GGTCCCCGCGCCCGGCCCGCGCGAGGTCCGCGTGCGTGTGCGGCGGGTGGGCGTGTGCGGCAGCGACGTGCACTACTACA
CCCATGGCCGGATCGGCTCTTTTGTGGTGGAGGCGCCGCTGATCCTCGGCCATGAGGTAATGGGCGTGGTGGACGCGGTG
GGCGAGGGCGTCACCCACGTCCGGCCTGGCGACCGGGTGGCGCTGGAACCTGGTGTGCCGTGCCGCCGCTGCGCTTTCTG
TAAGCGCGGCGAGTACAACCTCTGCCCCGACATGACCTTTATGGCGACCCCGCCCGTCCACGGCGCGCTCGGTGAATACG
TGCTTTGGCCCGACGACTTTGCGTTTCTGCTGCCTGACCGCATCAGTGACGACGCGGGCGCCCTCCTCGAACCGCTCGCG
GTCGGGATCTGGGCCGCGCGCAAGGGCGACGTGCGTCCTGGGCAAAGCGTGGCGGTCTTTGGCGCCGGTCCCATCGGCTG
CACCACCCTTCAAGCCGCCAAGGCGGCCGGGGCCACCACCCTCATTGCTGTTGATCTGGAAGACTTCCGGCTCGACCTGG
CGAGAAAGGTAGGGGCCACCCACACCATCAACGCCCGCAACGAGGACCCCGTCGCTCGCATTCGCGAGATCACGCGCGGC
GACCTGCCGATCTCCCACGCCGGAGTGGATGTGGCCTTTGAGACGGCAGGGAGCCTCCCCACCACTCGCATGAGCCTCGC
CGCGCCCCGCCCAGGCGGCACCACTGTGTTGGTGGGCCTGCCGCCCGACTCCGAAGTCAGCCTCGACATCGTCTCGGCAG
CCAGCCGCGAGGTGAGCATCCGGGGTGTTTTCCGCTATGCGAACTGCTATCCGGCGGCCATTGCGCTGGTGGAGAGCGGC
GCGGTGGACCTCGACGTGCTTGTCACCCACCGCTATCCCTTTGACCAGACCCCCGAAGCTTTTGCGTTCGCTGACCGTGA
GAAACGCGCCAGCATGAAGGTCATGATCGATGTCGGCTGA

Upstream 100 bases:

>100_bases
CTCGCCAGCGACGCCGCCAGCTTCGTCACCGGGCATACGCTCGTGGTGGACGGTGGGTACACGGTGTGGTGAGGGTTTTC
CCGATCAGAAAGGCAGGACG

Downstream 100 bases:

>100_bases
GCTGCTCATCGGGATCGACGTGGGCACATACTCCAGCAAGGGCGTGCTGGTGACGCTGGATGGCCAAATCCTGCGCCAGC
ACGTCATCCCCCACGGCATC

Product: alcohol dehydrogenase GroES-like protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTSPDSSLSSRISVLHGIRDLRWETREVPAPGPREVRVRVRRVGVCGSDVHYYTHGRIGSFVVEAPLILGHEVMGVVDAV
GEGVTHVRPGDRVALEPGVPCRRCAFCKRGEYNLCPDMTFMATPPVHGALGEYVLWPDDFAFLLPDRISDDAGALLEPLA
VGIWAARKGDVRPGQSVAVFGAGPIGCTTLQAAKAAGATTLIAVDLEDFRLDLARKVGATHTINARNEDPVARIREITRG
DLPISHAGVDVAFETAGSLPTTRMSLAAPRPGGTTVLVGLPPDSEVSLDIVSAASREVSIRGVFRYANCYPAAIALVESG
AVDLDVLVTHRYPFDQTPEAFAFADREKRASMKVMIDVG

Sequences:

>Translated_359_residues
MTSPDSSLSSRISVLHGIRDLRWETREVPAPGPREVRVRVRRVGVCGSDVHYYTHGRIGSFVVEAPLILGHEVMGVVDAV
GEGVTHVRPGDRVALEPGVPCRRCAFCKRGEYNLCPDMTFMATPPVHGALGEYVLWPDDFAFLLPDRISDDAGALLEPLA
VGIWAARKGDVRPGQSVAVFGAGPIGCTTLQAAKAAGATTLIAVDLEDFRLDLARKVGATHTINARNEDPVARIREITRG
DLPISHAGVDVAFETAGSLPTTRMSLAAPRPGGTTVLVGLPPDSEVSLDIVSAASREVSIRGVFRYANCYPAAIALVESG
AVDLDVLVTHRYPFDQTPEAFAFADREKRASMKVMIDVG
>Mature_358_residues
TSPDSSLSSRISVLHGIRDLRWETREVPAPGPREVRVRVRRVGVCGSDVHYYTHGRIGSFVVEAPLILGHEVMGVVDAVG
EGVTHVRPGDRVALEPGVPCRRCAFCKRGEYNLCPDMTFMATPPVHGALGEYVLWPDDFAFLLPDRISDDAGALLEPLAV
GIWAARKGDVRPGQSVAVFGAGPIGCTTLQAAKAAGATTLIAVDLEDFRLDLARKVGATHTINARNEDPVARIREITRGD
LPISHAGVDVAFETAGSLPTTRMSLAAPRPGGTTVLVGLPPDSEVSLDIVSAASREVSIRGVFRYANCYPAAIALVESGA
VDLDVLVTHRYPFDQTPEAFAFADREKRASMKVMIDVG

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=346, Percent_Identity=44.2196531791908, Blast_Score=284, Evalue=8e-77,
Organism=Homo sapiens, GI4501929, Length=374, Percent_Identity=32.0855614973262, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI4501933, Length=373, Percent_Identity=31.0991957104558, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI34577061, Length=373, Percent_Identity=31.0991957104558, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI262073058, Length=375, Percent_Identity=28.5333333333333, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI71743840, Length=349, Percent_Identity=29.2263610315186, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI71565152, Length=367, Percent_Identity=28.0653950953678, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4501939, Length=344, Percent_Identity=29.6511627906977, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI156523966, Length=344, Percent_Identity=29.6511627906977, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI71565154, Length=380, Percent_Identity=27.6315789473684, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1788073, Length=344, Percent_Identity=41.5697674418605, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1790718, Length=324, Percent_Identity=30.5555555555556, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1790045, Length=324, Percent_Identity=32.4074074074074, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787863, Length=336, Percent_Identity=31.25, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI226510992, Length=330, Percent_Identity=30.6060606060606, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1788407, Length=329, Percent_Identity=31.0030395136778, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788075, Length=345, Percent_Identity=28.4057971014493, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786825, Length=382, Percent_Identity=27.4869109947644, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87082125, Length=331, Percent_Identity=30.2114803625378, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786552, Length=350, Percent_Identity=31.4285714285714, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87081918, Length=258, Percent_Identity=34.4961240310078, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786518, Length=214, Percent_Identity=29.9065420560748, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=346, Percent_Identity=47.9768786127168, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17562878, Length=329, Percent_Identity=47.7203647416413, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25146526, Length=359, Percent_Identity=27.2980501392758, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71988145, Length=286, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562584, Length=291, Percent_Identity=32.3024054982818, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=284, Percent_Identity=27.112676056338, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323099, Length=326, Percent_Identity=39.8773006134969, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319955, Length=342, Percent_Identity=39.4736842105263, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6322619, Length=342, Percent_Identity=39.4736842105263, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6320033, Length=354, Percent_Identity=28.5310734463277, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319257, Length=362, Percent_Identity=27.9005524861878, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319258, Length=365, Percent_Identity=27.1232876712329, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319621, Length=287, Percent_Identity=30.6620209059233, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=312, Percent_Identity=27.5641025641026, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=283, Percent_Identity=28.9752650176678, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17737897, Length=351, Percent_Identity=46.4387464387464, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI17137530, Length=349, Percent_Identity=46.1318051575931, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI221457811, Length=359, Percent_Identity=30.6406685236769, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45551930, Length=359, Percent_Identity=30.6406685236769, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550770, Length=359, Percent_Identity=30.6406685236769, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17737895, Length=350, Percent_Identity=28.8571428571429, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 38427; Mature: 38296

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPDSSLSSRISVLHGIRDLRWETREVPAPGPREVRVRVRRVGVCGSDVHYYTHGRIGS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCCEEEECCCCCC
FVVEAPLILGHEVMGVVDAVGEGVTHVRPGDRVALEPGVPCRRCAFCKRGEYNLCPDMTF
CEECCHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCHHHHHHHCCCCCCCCCCCEE
MATPPVHGALGEYVLWPDDFAFLLPDRISDDAGALLEPLAVGIWAARKGDVRPGQSVAVF
EECCCCCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEE
GAGPIGCTTLQAAKAAGATTLIAVDLEDFRLDLARKVGATHTINARNEDPVARIREITRG
ECCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCC
DLPISHAGVDVAFETAGSLPTTRMSLAAPRPGGTTVLVGLPPDSEVSLDIVSAASREVSI
CCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCCEEEE
RGVFRYANCYPAAIALVESGAVDLDVLVTHRYPFDQTPEAFAFADREKRASMKVMIDVG
EEEEEECCCCHHHHEEECCCCCEEEEEEEECCCCCCCCCHHEECCCHHCCCEEEEEEEC
>Mature Secondary Structure 
TSPDSSLSSRISVLHGIRDLRWETREVPAPGPREVRVRVRRVGVCGSDVHYYTHGRIGS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCCEEEECCCCCC
FVVEAPLILGHEVMGVVDAVGEGVTHVRPGDRVALEPGVPCRRCAFCKRGEYNLCPDMTF
CEECCHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCHHHHHHHCCCCCCCCCCCEE
MATPPVHGALGEYVLWPDDFAFLLPDRISDDAGALLEPLAVGIWAARKGDVRPGQSVAVF
EECCCCCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEE
GAGPIGCTTLQAAKAAGATTLIAVDLEDFRLDLARKVGATHTINARNEDPVARIREITRG
ECCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCC
DLPISHAGVDVAFETAGSLPTTRMSLAAPRPGGTTVLVGLPPDSEVSLDIVSAASREVSI
CCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCCEEEE
RGVFRYANCYPAAIALVESGAVDLDVLVTHRYPFDQTPEAFAFADREKRASMKVMIDVG
EEEEEECCCCHHHHEEECCCCCEEEEEEEECCCCCCCCCHHEECCCHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]