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The map label for this gene is lacF [H]

Identifier: 94972294

GI number: 94972294

Start: 194041

End: 194904

Strand: Direct

Name: lacF [H]

Synonym: Dgeo_2831

Alternate gene names: 94972294

Gene position: 194041-194904 (Clockwise)

Preceding gene: 94972295

Following gene: 94972293

Centisome position: 33.8

GC content: 62.73

Gene sequence:

>864_bases
ATGAAGGTGCCGCGCCTCGTGCCGTGGCTGTTCCTGTCCCCGTTCCTGTTGCTGTTCGCGGCGTTCTACCTGGCGCCGGT
GTTGTACGCCGGGTATCTCAGCCTCTTTATCAAAAAGCGGAGCGGCTTTGGCCCGGCCCACGATGTGTTCGGCGGCCTGA
CCAACTACGTTCGGGCTTTTGGGGACAGTGATTTTCTCGCCAGCCTGCTGAATATCCTCAAATTTGGTGTGGTGCAGGTG
CCGCTGATGCTTGTGCTGGCCACCGCCCTCGCGCTGATCCTCGACGGCGTGAAGGGACCGCTCCAGGGCTTTTTTCGCAC
CGCCTTCTACCTGCCGTACACGATTCCCAGCGTGATCGCAGGCCTGCTGTGGGGTTACCTCTACAGCAAGAATCTCTCGC
CCTTCAATCAGCTCACCGGCCTCCAGACCGACTTCCTGTCGAGCGGCGTGGTGCTGTGGAGCATCGCCAACATCGTGACC
TGGACCTGGACGGGCTACAACATGATCACCCTGTACGCCGCGTTGCAGAACATTCCCGCCGAGCTGTACGAGGCGGCCCG
GATCGACGGCGCGTCGAACTGGACGCTGACGCGCTTCATCAAGCTGCCGTTGCTGCGGCCCAGCCTGCTGCTGGTGCTGA
TCTTCTCGATCATCGGCACGATGCAGATTTTCGCCGAGCCGTTCGTGCTGCGGCCCCTGGGCTATGTGCCCGATCACATC
ACGCCGAACACCTACCTGTATCTGGTGGCCAGCCGCGACGGCAACTTCAGCTACGCGGCGGCGCTGGCCCTCCTGCTGGC
GGTCTTCACGTTCCTGCTCAGTGCCGTCTTTCTGCGCCTGACCCGCCGGGGAGGGGCGCTGTGA

Upstream 100 bases:

>100_bases
GGGGGCCGGAAGCGCTGGCCGTCTCCGGCACCCCCTTGACACGCCCGTCCCTTTCCCTTGCCAGAGATCAGGCCATGTCC
ATCCCCCCGGGAGGCTCCCC

Downstream 100 bases:

>100_bases
CTCTGTCGCGTTCTGTCCCGGCGCAGGTGGCCCCGCGCGCTCGGTCCCGGCCCGGCTTCACGCCGCTGCAGCTGCTTGCG
CTGGTCCTCTTCGCCGCCTA

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKVPRLVPWLFLSPFLLLFAAFYLAPVLYAGYLSLFIKKRSGFGPAHDVFGGLTNYVRAFGDSDFLASLLNILKFGVVQV
PLMLVLATALALILDGVKGPLQGFFRTAFYLPYTIPSVIAGLLWGYLYSKNLSPFNQLTGLQTDFLSSGVVLWSIANIVT
WTWTGYNMITLYAALQNIPAELYEAARIDGASNWTLTRFIKLPLLRPSLLLVLIFSIIGTMQIFAEPFVLRPLGYVPDHI
TPNTYLYLVASRDGNFSYAAALALLLAVFTFLLSAVFLRLTRRGGAL

Sequences:

>Translated_287_residues
MKVPRLVPWLFLSPFLLLFAAFYLAPVLYAGYLSLFIKKRSGFGPAHDVFGGLTNYVRAFGDSDFLASLLNILKFGVVQV
PLMLVLATALALILDGVKGPLQGFFRTAFYLPYTIPSVIAGLLWGYLYSKNLSPFNQLTGLQTDFLSSGVVLWSIANIVT
WTWTGYNMITLYAALQNIPAELYEAARIDGASNWTLTRFIKLPLLRPSLLLVLIFSIIGTMQIFAEPFVLRPLGYVPDHI
TPNTYLYLVASRDGNFSYAAALALLLAVFTFLLSAVFLRLTRRGGAL
>Mature_287_residues
MKVPRLVPWLFLSPFLLLFAAFYLAPVLYAGYLSLFIKKRSGFGPAHDVFGGLTNYVRAFGDSDFLASLLNILKFGVVQV
PLMLVLATALALILDGVKGPLQGFFRTAFYLPYTIPSVIAGLLWGYLYSKNLSPFNQLTGLQTDFLSSGVVLWSIANIVT
WTWTGYNMITLYAALQNIPAELYEAARIDGASNWTLTRFIKLPLLRPSLLLVLIFSIIGTMQIFAEPFVLRPLGYVPDHI
TPNTYLYLVASRDGNFSYAAALALLLAVFTFLLSAVFLRLTRRGGAL

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=228, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787570, Length=246, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790465, Length=263, Percent_Identity=28.5171102661597, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31812; Mature: 31812

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVPRLVPWLFLSPFLLLFAAFYLAPVLYAGYLSLFIKKRSGFGPAHDVFGGLTNYVRAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GDSDFLASLLNILKFGVVQVPLMLVLATALALILDGVKGPLQGFFRTAFYLPYTIPSVIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GLLWGYLYSKNLSPFNQLTGLQTDFLSSGVVLWSIANIVTWTWTGYNMITLYAALQNIPA
HHHHHHHHHCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
ELYEAARIDGASNWTLTRFIKLPLLRPSLLLVLIFSIIGTMQIFAEPFVLRPLGYVPDHI
HHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
TPNTYLYLVASRDGNFSYAAALALLLAVFTFLLSAVFLRLTRRGGAL
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVPRLVPWLFLSPFLLLFAAFYLAPVLYAGYLSLFIKKRSGFGPAHDVFGGLTNYVRAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GDSDFLASLLNILKFGVVQVPLMLVLATALALILDGVKGPLQGFFRTAFYLPYTIPSVIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GLLWGYLYSKNLSPFNQLTGLQTDFLSSGVVLWSIANIVTWTWTGYNMITLYAALQNIPA
HHHHHHHHHCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
ELYEAARIDGASNWTLTRFIKLPLLRPSLLLVLIFSIIGTMQIFAEPFVLRPLGYVPDHI
HHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
TPNTYLYLVASRDGNFSYAAALALLLAVFTFLLSAVFLRLTRRGGAL
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]