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The map label for this gene is yfkN [H]

Identifier: 94972218

GI number: 94972218

Start: 273603

End: 275165

Strand: Direct

Name: yfkN [H]

Synonym: Dgeo_2751

Alternate gene names: 94972218

Gene position: 273603-275165 (Clockwise)

Preceding gene: 94972219

Following gene: 94972213

Centisome position: 47.66

GC content: 62.12

Gene sequence:

>1563_bases
ATGAAATCCTGGTTCCTCGCCGCTGCCCTTCTGACCTCCGTCGCGGGCGCCGCGCCCCTCACCGTGACCATCCTGCACAC
CGACGACCTGCACGGGCACCTCGATCCCGTCAAGGTCGGCTCGGGCACCTACGGCGGCTACGCGCGCCAAACCGCACTGG
TCCACAAGTATGCGGCGCAGGATCCCAATCCGCTTGTCCTCTCGGGCGGCGACACCTTCCAAGGCACGCTCTTTTACAAC
GTCTACCAGGGCCTGGCCGACGTATTGTTTATGAACCTGCAGGGCTATCAGGCGATGGCAGTGGGCAACCACGAGTTCGA
CAATGGCCCTGAGGCGCTCGCTCGCTTTGCCCAAAAGGCGCAGTTTCCGCTCCTGGCTGCCAACCTTGACCTCAGCCAAG
AACCGCGGCTGAAGGACCTGATCAAACCCTACGCGGTCCTGAACGTGGGCGGGCAGAAGGTGGGGGTGATCGGCGCGGTG
ACCCCCGACCTCCCGCTGATCAGTTCTCCCGGCGACAACGTGAAGATGCTGGAACTGATGCAGAGCCTGAAAAACAGCGT
AGAGGCGCTTCAGGCCCAGGGCATCAACAAAATCATCCTGCTGTCGCACCTGGGCTACACCCTGGAGCAGGAGGTGGCCA
AGACGGTGCCCGGCATTGATGTGATTGTGGGCGGGCATTCTCATACCCTCTTAGGGACCTTTGACAACAAGGACTTTCCC
AAGAGCGAGGGACCCTACCCCACGGTGGTGCAGAACCCCGACGGCAACCGCACGCTGCTGGTCGCCGCCTGGGAATGGGG
CAAGGTGCTGGGCCGCCTCAAGGTCACCTTCGATGATCAAGGCGCGGTGACGAGCTACGAGGGCAACCCGATCCCCGTGT
CGGCTGACCTGCCGGAAGACCCCACCGCCAAGCGCATGATCGAGACGCTGAGTGTGCCCATCGCCAATCTGCGCAAGCAA
GTGATCGGCAATACACCGGGAGGCCTCAACGGCAGCCGCGAGATCGTCCGCAAACGTGAGAGCGGTATGGCGAACGTGCT
GGCCGACGCGGCGCTCGAAGCCGGGCAGAAAGCGGGCGCTGTCATCGCCTTTGTGAATGGCGGCGGCGTGCGTGCCAGCA
TCGATCCAGGCCCGATCACCTTTGAAGAAGCGATCACGGTTCAGCCTTTCGGCAATACGCTAACGATCCTTGATCTCACC
GGTGCCGAAATCAAGCAGGCGCTCGAGCATGGCGTTGCGACCTGGAGCGAGAGCAAGGGCCAGTTTCTGCACGTCTCCAA
GGGCATGAGCTACACCTTCGACCTCTCCAAGCCTGCCGGGAGCCGCGTCACCTCGGTCACGCTGAATGGCCAGCCCCTCG
AGGACAACAAAACCTACACGGTTGCGGTGAATACCTTCACGGCCAACGGCGGCGACGGCTTCGACGTGCTCAAAAACGCC
AAGGGCCGCCGCATCGACACAGGCCTCCTCGACATCGACATTCTGGTGAACTACTTCAAGACGCATCCGACGGTTGATGC
CCAGCCGGAAGGGCGCATCGTGATTCAGAACGAACCGAAGTAA

Upstream 100 bases:

>100_bases
GACAACCGGGCACGTTGACCCGCCACGACCCCAACAAGGAACGCTTCAGGCGCTCGCACGGTGTGACAGCGGCAGCAGTA
CACCTTATGATGAGGTGGTG

Downstream 100 bases:

>100_bases
GCCGGGCGCGGGTCGAAAGAAGGTCGTACGGAAAAGTTTACAGTGCTTTTGGTCCCTGAACTGCCCGCCTGGCCCAGCAG
TGAGGCCCGAAGACCGCTCG

Product: 5'-nucleotidase-like

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKSWFLAAALLTSVAGAAPLTVTILHTDDLHGHLDPVKVGSGTYGGYARQTALVHKYAAQDPNPLVLSGGDTFQGTLFYN
VYQGLADVLFMNLQGYQAMAVGNHEFDNGPEALARFAQKAQFPLLAANLDLSQEPRLKDLIKPYAVLNVGGQKVGVIGAV
TPDLPLISSPGDNVKMLELMQSLKNSVEALQAQGINKIILLSHLGYTLEQEVAKTVPGIDVIVGGHSHTLLGTFDNKDFP
KSEGPYPTVVQNPDGNRTLLVAAWEWGKVLGRLKVTFDDQGAVTSYEGNPIPVSADLPEDPTAKRMIETLSVPIANLRKQ
VIGNTPGGLNGSREIVRKRESGMANVLADAALEAGQKAGAVIAFVNGGGVRASIDPGPITFEEAITVQPFGNTLTILDLT
GAEIKQALEHGVATWSESKGQFLHVSKGMSYTFDLSKPAGSRVTSVTLNGQPLEDNKTYTVAVNTFTANGGDGFDVLKNA
KGRRIDTGLLDIDILVNYFKTHPTVDAQPEGRIVIQNEPK

Sequences:

>Translated_520_residues
MKSWFLAAALLTSVAGAAPLTVTILHTDDLHGHLDPVKVGSGTYGGYARQTALVHKYAAQDPNPLVLSGGDTFQGTLFYN
VYQGLADVLFMNLQGYQAMAVGNHEFDNGPEALARFAQKAQFPLLAANLDLSQEPRLKDLIKPYAVLNVGGQKVGVIGAV
TPDLPLISSPGDNVKMLELMQSLKNSVEALQAQGINKIILLSHLGYTLEQEVAKTVPGIDVIVGGHSHTLLGTFDNKDFP
KSEGPYPTVVQNPDGNRTLLVAAWEWGKVLGRLKVTFDDQGAVTSYEGNPIPVSADLPEDPTAKRMIETLSVPIANLRKQ
VIGNTPGGLNGSREIVRKRESGMANVLADAALEAGQKAGAVIAFVNGGGVRASIDPGPITFEEAITVQPFGNTLTILDLT
GAEIKQALEHGVATWSESKGQFLHVSKGMSYTFDLSKPAGSRVTSVTLNGQPLEDNKTYTVAVNTFTANGGDGFDVLKNA
KGRRIDTGLLDIDILVNYFKTHPTVDAQPEGRIVIQNEPK
>Mature_520_residues
MKSWFLAAALLTSVAGAAPLTVTILHTDDLHGHLDPVKVGSGTYGGYARQTALVHKYAAQDPNPLVLSGGDTFQGTLFYN
VYQGLADVLFMNLQGYQAMAVGNHEFDNGPEALARFAQKAQFPLLAANLDLSQEPRLKDLIKPYAVLNVGGQKVGVIGAV
TPDLPLISSPGDNVKMLELMQSLKNSVEALQAQGINKIILLSHLGYTLEQEVAKTVPGIDVIVGGHSHTLLGTFDNKDFP
KSEGPYPTVVQNPDGNRTLLVAAWEWGKVLGRLKVTFDDQGAVTSYEGNPIPVSADLPEDPTAKRMIETLSVPIANLRKQ
VIGNTPGGLNGSREIVRKRESGMANVLADAALEAGQKAGAVIAFVNGGGVRASIDPGPITFEEAITVQPFGNTLTILDLT
GAEIKQALEHGVATWSESKGQFLHVSKGMSYTFDLSKPAGSRVTSVTLNGQPLEDNKTYTVAVNTFTANGGDGFDVLKNA
KGRRIDTGLLDIDILVNYFKTHPTVDAQPEGRIVIQNEPK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=536, Percent_Identity=37.1268656716418, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1786687, Length=539, Percent_Identity=30.7977736549165, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1790658, Length=548, Percent_Identity=23.1751824817518, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922446, Length=532, Percent_Identity=34.9624060150376, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24654424, Length=532, Percent_Identity=34.9624060150376, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI28573524, Length=533, Percent_Identity=31.3320825515947, Blast_Score=268, Evalue=9e-72,
Organism=Drosophila melanogaster, GI24641187, Length=532, Percent_Identity=31.203007518797, Blast_Score=248, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19922444, Length=539, Percent_Identity=31.7254174397032, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI221329836, Length=526, Percent_Identity=31.3688212927757, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI19921980, Length=495, Percent_Identity=26.6666666666667, Blast_Score=139, Evalue=7e-33,
Organism=Drosophila melanogaster, GI161076508, Length=495, Percent_Identity=26.6666666666667, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24652512, Length=495, Percent_Identity=26.6666666666667, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 55452; Mature: 55452

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWFLAAALLTSVAGAAPLTVTILHTDDLHGHLDPVKVGSGTYGGYARQTALVHKYAAQ
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCC
DPNPLVLSGGDTFQGTLFYNVYQGLADVLFMNLQGYQAMAVGNHEFDNGPEALARFAQKA
CCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHC
QFPLLAANLDLSQEPRLKDLIKPYAVLNVGGQKVGVIGAVTPDLPLISSPGDNVKMLELM
CCCEEEECCCCCCCCCHHHHHCCEEEEECCCCEEEEEEECCCCCCEECCCCCCHHHHHHH
QSLKNSVEALQAQGINKIILLSHLGYTLEQEVAKTVPGIDVIVGGHSHTLLGTFDNKDFP
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCC
KSEGPYPTVVQNPDGNRTLLVAAWEWGKVLGRLKVTFDDQGAVTSYEGNPIPVSADLPED
CCCCCCCCEEECCCCCEEEEEEEECHHHEEEEEEEEECCCCCEEECCCCEEEEECCCCCC
PTAKRMIETLSVPIANLRKQVIGNTPGGLNGSREIVRKRESGMANVLADAALEAGQKAGA
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCE
VIAFVNGGGVRASIDPGPITFEEAITVQPFGNTLTILDLTGAEIKQALEHGVATWSESKG
EEEEEECCCEEEECCCCCEEHHHEEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCCC
QFLHVSKGMSYTFDLSKPAGSRVTSVTLNGQPLEDNKTYTVAVNTFTANGGDGFDVLKNA
CEEEEECCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCHHHHHCC
KGRRIDTGLLDIDILVNYFKTHPTVDAQPEGRIVIQNEPK
CCCEEECCEEEHHHHHHHHHCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKSWFLAAALLTSVAGAAPLTVTILHTDDLHGHLDPVKVGSGTYGGYARQTALVHKYAAQ
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCC
DPNPLVLSGGDTFQGTLFYNVYQGLADVLFMNLQGYQAMAVGNHEFDNGPEALARFAQKA
CCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHC
QFPLLAANLDLSQEPRLKDLIKPYAVLNVGGQKVGVIGAVTPDLPLISSPGDNVKMLELM
CCCEEEECCCCCCCCCHHHHHCCEEEEECCCCEEEEEEECCCCCCEECCCCCCHHHHHHH
QSLKNSVEALQAQGINKIILLSHLGYTLEQEVAKTVPGIDVIVGGHSHTLLGTFDNKDFP
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCC
KSEGPYPTVVQNPDGNRTLLVAAWEWGKVLGRLKVTFDDQGAVTSYEGNPIPVSADLPED
CCCCCCCCEEECCCCCEEEEEEEECHHHEEEEEEEEECCCCCEEECCCCEEEEECCCCCC
PTAKRMIETLSVPIANLRKQVIGNTPGGLNGSREIVRKRESGMANVLADAALEAGQKAGA
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCE
VIAFVNGGGVRASIDPGPITFEEAITVQPFGNTLTILDLTGAEIKQALEHGVATWSESKG
EEEEEECCCEEEECCCCCEEHHHEEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCCC
QFLHVSKGMSYTFDLSKPAGSRVTSVTLNGQPLEDNKTYTVAVNTFTANGGDGFDVLKNA
CEEEEECCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCHHHHHCC
KGRRIDTGLLDIDILVNYFKTHPTVDAQPEGRIVIQNEPK
CCCEEECCEEEHHHHHHHHHCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]