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The map label for this gene is leuD [H]

Identifier: 94314786

GI number: 94314786

Start: 62643

End: 63155

Strand: Reverse

Name: leuD [H]

Synonym: Rmet_5867

Alternate gene names: 94314786

Gene position: 63155-62643 (Counterclockwise)

Preceding gene: 94314787

Following gene: 94314784

Centisome position: 2.45

GC content: 66.28

Gene sequence:

>513_bases
ATGACCCCGGCTCCGGATTTCCACCCCCCGCCCTCTGGCCGCATCTGGCGCTTCGGCGAAAACGTCGATACCGACGCGAT
GGCGCCGGGCCGATTCATGAAGGACGGGCTCGATGTGCTGGCAAGCCACTGCCTGGAAAACCTGCGGCCGGAGTTTCCGG
GCGCGGTCAAACCCGGCGACGTCATCGTGGCGGGCAGCAACTTCGGCATGGGGTCTTCGCGTGAGCAGGCAGCCCAGGCA
TTGAAGCATCTTGGCGTGGCGGCCGTGCTGGCGTCGTCGTTCGCCGGGCTGTTTTATCGCAACGCGATCAACATTGGGCT
GCCGGTGCTGGTGTGCGCGGATACGGCCGCATTGGCCGACGGAGCGCGAGCCACGTTCTTCCTCGATGCGGCCGAGATCA
GGCTCTGCGATGGCCGGCGCGTATCCTGCGAACCGGTGCCCGATTTCCTGCGCGCGCTGTTGCACGCGGGCGGGCTGGTG
CCGCACCTCAAAGCCCGCCTCAATAAAGTCTGA

Upstream 100 bases:

>100_bases
GCATGGGCGATGCAGGTAGCGCCGTATGGCTGGCATCGCCCATGACCGTGGCTGCCAGCGCCGCCACCGGCTGCATCACT
GACCCACGCACGTTACTGGC

Downstream 100 bases:

>100_bases
GACCCTGGTGTCAGTGGGCAGCCGGCCCGTTGCTGAGGTGTCCTGACTCACCACCTCCTGACACACCACCATTGCGCGAC
CAGGCACCATGCGTGGCCAG

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTPAPDFHPPPSGRIWRFGENVDTDAMAPGRFMKDGLDVLASHCLENLRPEFPGAVKPGDVIVAGSNFGMGSSREQAAQA
LKHLGVAAVLASSFAGLFYRNAINIGLPVLVCADTAALADGARATFFLDAAEIRLCDGRRVSCEPVPDFLRALLHAGGLV
PHLKARLNKV

Sequences:

>Translated_170_residues
MTPAPDFHPPPSGRIWRFGENVDTDAMAPGRFMKDGLDVLASHCLENLRPEFPGAVKPGDVIVAGSNFGMGSSREQAAQA
LKHLGVAAVLASSFAGLFYRNAINIGLPVLVCADTAALADGARATFFLDAAEIRLCDGRRVSCEPVPDFLRALLHAGGLV
PHLKARLNKV
>Mature_169_residues
TPAPDFHPPPSGRIWRFGENVDTDAMAPGRFMKDGLDVLASHCLENLRPEFPGAVKPGDVIVAGSNFGMGSSREQAAQAL
KHLGVAAVLASSFAGLFYRNAINIGLPVLVCADTAALADGARATFFLDAAEIRLCDGRRVSCEPVPDFLRALLHAGGLVP
HLKARLNKV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=95, Percent_Identity=44.2105263157895, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18017; Mature: 17886

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAPDFHPPPSGRIWRFGENVDTDAMAPGRFMKDGLDVLASHCLENLRPEFPGAVKPGD
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VIVAGSNFGMGSSREQAAQALKHLGVAAVLASSFAGLFYRNAINIGLPVLVCADTAALAD
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
GARATFFLDAAEIRLCDGRRVSCEPVPDFLRALLHAGGLVPHLKARLNKV
CCCEEEEEEHHHEEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TPAPDFHPPPSGRIWRFGENVDTDAMAPGRFMKDGLDVLASHCLENLRPEFPGAVKPGD
CCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VIVAGSNFGMGSSREQAAQALKHLGVAAVLASSFAGLFYRNAINIGLPVLVCADTAALAD
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
GARATFFLDAAEIRLCDGRRVSCEPVPDFLRALLHAGGLVPHLKARLNKV
CCCEEEEEEHHHEEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]