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The map label for this gene is iciA [H]

Identifier: 94312791

GI number: 94312791

Start: 406232

End: 407128

Strand: Direct

Name: iciA [H]

Synonym: Rmet_3863

Alternate gene names: 94312791

Gene position: 406232-407128 (Clockwise)

Preceding gene: 94312785

Following gene: 94312796

Centisome position: 15.74

GC content: 69.45

Gene sequence:

>897_bases
ATGCTGGACTACGCCGCGCTTTCCGCACTGGCTGCTGTGATTCGCGAGGGCAGCTTCGAGCGTGCGGCTCGCGCGCTCAA
CGTGACGCCGTCGGCGATTTCGCAGCGCATCCGCTTGCTGGAAGAGCGCGTGGGCTGCGCGCTGGTGGTGCGTGCCCAGC
CTTGCCGGGCGACCGAGACCGGACGGCGGTTGTGCCAGCACGTGGACCGGGTGCGTTTGCTTGAGCAGGAACTTCAGGGG
GCGCTGCCGGCGCTGGCGCCCGAGGGTGTGACACGGGTGGCGTTACCGGTGGCGGTGAATGCCGATAGCCTGGCCACCTG
GGCCGCGCCCGCCATTGCCACGTTCGCCGCGGCGCATCCCGTATTGATGGAAGTGGCCGTGGACGATCAGGACCACACCA
CCGAGTGGCTACGCAGCGGCGCAGTGCTGGCTGCCGTGACGGGCACCGGCCGGCCCGCGGCAGGATGCAACAGCCGCCCG
CTTGGCGCGATGCGCTATCTGGCGGCGGCCAGTCCGGCGTTCATGCAACGCTACTTTGTCGATGGCGTTGGCGCAGGCAC
TCTGGCCGAGGCGCCGAGCCTCGTGTTCAACACCAAGGATGAGCTGCAGGTGCGGTGGGCGCGGCGCTTGTGCCATCGGC
ATGTGGAACTGCCCCGGCATACGTTGCCGTCGTCGCAGGCGTTCGTGACGGCGGCCTTGGCTGGCATGGGCTGGGGCTTG
CATCCACAGGCGCTCGTCGCGCCGTATCTGGCGAATGGCACGCTGGTCGAACTGGTGCCAGGGGCGCCGCTCGATGTCCC
CCTTCACTGGCAATACGCACGCGCGGCGTCAGGCTTGCTCGACGGGCTGAGCCGCGAGGTGCTGGCTGCCGCGCGGGTTG
CGCTACTGGCGCCCTGA

Upstream 100 bases:

>100_bases
GATGGGGCGGGGAGGGCGGTGATCATCCGAGGGAGCTTGCCTGTCTTTCTGGATGAAGACAAACTATCTTTCCTTCATCT
AATTAAGTTGAGCTAAACCG

Downstream 100 bases:

>100_bases
TCTTGGCTGGTGTTGGCTGGGGGCGGCGGGTTCCCGTATGGCCTGCGGCAGCCATGCTTCGACAAGACATGGAAGCCGGG
GATATCGACGTGGTGAAGCA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLDYAALSALAAVIREGSFERAARALNVTPSAISQRIRLLEERVGCALVVRAQPCRATETGRRLCQHVDRVRLLEQELQG
ALPALAPEGVTRVALPVAVNADSLATWAAPAIATFAAAHPVLMEVAVDDQDHTTEWLRSGAVLAAVTGTGRPAAGCNSRP
LGAMRYLAAASPAFMQRYFVDGVGAGTLAEAPSLVFNTKDELQVRWARRLCHRHVELPRHTLPSSQAFVTAALAGMGWGL
HPQALVAPYLANGTLVELVPGAPLDVPLHWQYARAASGLLDGLSREVLAAARVALLAP

Sequences:

>Translated_298_residues
MLDYAALSALAAVIREGSFERAARALNVTPSAISQRIRLLEERVGCALVVRAQPCRATETGRRLCQHVDRVRLLEQELQG
ALPALAPEGVTRVALPVAVNADSLATWAAPAIATFAAAHPVLMEVAVDDQDHTTEWLRSGAVLAAVTGTGRPAAGCNSRP
LGAMRYLAAASPAFMQRYFVDGVGAGTLAEAPSLVFNTKDELQVRWARRLCHRHVELPRHTLPSSQAFVTAALAGMGWGL
HPQALVAPYLANGTLVELVPGAPLDVPLHWQYARAASGLLDGLSREVLAAARVALLAP
>Mature_298_residues
MLDYAALSALAAVIREGSFERAARALNVTPSAISQRIRLLEERVGCALVVRAQPCRATETGRRLCQHVDRVRLLEQELQG
ALPALAPEGVTRVALPVAVNADSLATWAAPAIATFAAAHPVLMEVAVDDQDHTTEWLRSGAVLAAVTGTGRPAAGCNSRP
LGAMRYLAAASPAFMQRYFVDGVGAGTLAEAPSLVFNTKDELQVRWARRLCHRHVELPRHTLPSSQAFVTAALAGMGWGL
HPQALVAPYLANGTLVELVPGAPLDVPLHWQYARAASGLLDGLSREVLAAARVALLAP

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=273, Percent_Identity=38.8278388278388, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31615; Mature: 31615

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYAALSALAAVIREGSFERAARALNVTPSAISQRIRLLEERVGCALVVRAQPCRATET
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHH
GRRLCQHVDRVRLLEQELQGALPALAPEGVTRVALPVAVNADSLATWAAPAIATFAAAHP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCH
VLMEVAVDDQDHTTEWLRSGAVLAAVTGTGRPAAGCNSRPLGAMRYLAAASPAFMQRYFV
HEEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
DGVGAGTLAEAPSLVFNTKDELQVRWARRLCHRHVELPRHTLPSSQAFVTAALAGMGWGL
HCCCCCHHHHCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
HPQALVAPYLANGTLVELVPGAPLDVPLHWQYARAASGLLDGLSREVLAAARVALLAP
CHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDYAALSALAAVIREGSFERAARALNVTPSAISQRIRLLEERVGCALVVRAQPCRATET
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHH
GRRLCQHVDRVRLLEQELQGALPALAPEGVTRVALPVAVNADSLATWAAPAIATFAAAHP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCH
VLMEVAVDDQDHTTEWLRSGAVLAAVTGTGRPAAGCNSRPLGAMRYLAAASPAFMQRYFV
HEEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
DGVGAGTLAEAPSLVFNTKDELQVRWARRLCHRHVELPRHTLPSSQAFVTAALAGMGWGL
HCCCCCHHHHCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
HPQALVAPYLANGTLVELVPGAPLDVPLHWQYARAASGLLDGLSREVLAAARVALLAP
CHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA