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The map label for this gene is flgJ [H]

Identifier: 94312674

GI number: 94312674

Start: 283972

End: 284994

Strand: Direct

Name: flgJ [H]

Synonym: Rmet_3742

Alternate gene names: 94312674

Gene position: 283972-284994 (Clockwise)

Preceding gene: 94312673

Following gene: 94312675

Centisome position: 11.01

GC content: 65.88

Gene sequence:

>1023_bases
ATGACGGGCGCGACGCTCTCCGGGCAGTCCGATATCTCGCAGCGCTTCGCGCTCGACACGCAAGGGTTCGAGGCGCTCAA
GAACCAGGCGCGCGCCGGCAACAACAGCGGTTCGCTGCAGGCCGCCGCCAGGCAGTTCGAAGCCGTGTTCACGAACATGG
TGCTGAAGAGCATGCGCGACGCGACGCCGTCTGACGGCCTCTTCGACAACGAGCAGACCAAGCTCTACATGTCGATGATG
GACCAGCAGCTGTCGCAACAGCTCTCCACACGTGGTATCGGCCTGGCCGATGTCATGATCCGCCAGCTTGCGCGCGCCAC
GGGCCAGCAACTACCGCCGAACATGGCTACGGCTGGCGGGGTCACCGCGGCAAGCATCCAGGACGCCCAGATGGCCGGGC
TGCTTGACGGCCGTGGCGCGACCGATGCCGATGGCCCGGCATTGCCGGTGGGCACGACGTTGCCCGGCCCGGCGTGGAAC
CCGACCGCCGGCGTGCGCCAGTACCAGAACCAGGCCGACTGGCAGGGCAATGGCCAGGCCCAACTGGGACAACTGCCCAA
CGACACACCCGAACACATCAGCAGCTTCGTCAACCGCATGGCAGGCCCGGCAATGGTCGCGTCGCGCGCATCGGGCGTGC
CGGCCAAGCTGATCGTTGGCCAGGCCGCGCTCGAATCGGGCTGGGGACGCCGCGAGATCCTGAATTCGGATGGCTCGACG
TCGTTCAACGTCTTCGGTATCAAGGCCGGTGCGAATTGGAAGGGCCCGACGACGGAGGTACTGACCACCGAATACGTCGA
TGGCCAGCCGCAGAAGGTGCGTGCCCGCTTCCGAGCCTATGGCTCCTACGAAGAGGCTTGTAATGACTATGCGCGGCTGC
TGACCTCCAATCCGCGTTACGCGGGCGTGGTGGCGGCAAACAGCGCCGACGAGGCCGCCCACGGCTTGCAGCGCGCGGGA
TATGCCACCGATCCGGCCTACGGCGAGAAGCTCGTCCGGATCATGAAGAAGGTTTCAACCTGA

Upstream 100 bases:

>100_bases
AGGGCCTGAACGCGCTTGGCGCGACCCCGGCCGACTTGCAGACGATCCTGGAGGCCATGCGTGCCGCGGGCGCCCTGCGC
GCCGAGCTGGAGATCATCTG

Downstream 100 bases:

>100_bases
AGCATCGCGATAGCGGGTTGGTCCCCTATGCCGCGTTGTGGGCGAGGGGAGAAAACAATCGGTGAACCTGCATGGCGCGG
CGCTTGTCAGTCGCGCTATT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTGATLSGQSDISQRFALDTQGFEALKNQARAGNNSGSLQAAARQFEAVFTNMVLKSMRDATPSDGLFDNEQTKLYMSMM
DQQLSQQLSTRGIGLADVMIRQLARATGQQLPPNMATAGGVTAASIQDAQMAGLLDGRGATDADGPALPVGTTLPGPAWN
PTAGVRQYQNQADWQGNGQAQLGQLPNDTPEHISSFVNRMAGPAMVASRASGVPAKLIVGQAALESGWGRREILNSDGST
SFNVFGIKAGANWKGPTTEVLTTEYVDGQPQKVRARFRAYGSYEEACNDYARLLTSNPRYAGVVAANSADEAAHGLQRAG
YATDPAYGEKLVRIMKKVST

Sequences:

>Translated_340_residues
MTGATLSGQSDISQRFALDTQGFEALKNQARAGNNSGSLQAAARQFEAVFTNMVLKSMRDATPSDGLFDNEQTKLYMSMM
DQQLSQQLSTRGIGLADVMIRQLARATGQQLPPNMATAGGVTAASIQDAQMAGLLDGRGATDADGPALPVGTTLPGPAWN
PTAGVRQYQNQADWQGNGQAQLGQLPNDTPEHISSFVNRMAGPAMVASRASGVPAKLIVGQAALESGWGRREILNSDGST
SFNVFGIKAGANWKGPTTEVLTTEYVDGQPQKVRARFRAYGSYEEACNDYARLLTSNPRYAGVVAANSADEAAHGLQRAG
YATDPAYGEKLVRIMKKVST
>Mature_339_residues
TGATLSGQSDISQRFALDTQGFEALKNQARAGNNSGSLQAAARQFEAVFTNMVLKSMRDATPSDGLFDNEQTKLYMSMMD
QQLSQQLSTRGIGLADVMIRQLARATGQQLPPNMATAGGVTAASIQDAQMAGLLDGRGATDADGPALPVGTTLPGPAWNP
TAGVRQYQNQADWQGNGQAQLGQLPNDTPEHISSFVNRMAGPAMVASRASGVPAKLIVGQAALESGWGRREILNSDGSTS
FNVFGIKAGANWKGPTTEVLTTEYVDGQPQKVRARFRAYGSYEEACNDYARLLTSNPRYAGVVAANSADEAAHGLQRAGY
ATDPAYGEKLVRIMKKVST

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=326, Percent_Identity=42.0245398773006, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 36027; Mature: 35895

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGATLSGQSDISQRFALDTQGFEALKNQARAGNNSGSLQAAARQFEAVFTNMVLKSMRD
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
ATPSDGLFDNEQTKLYMSMMDQQLSQQLSTRGIGLADVMIRQLARATGQQLPPNMATAGG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHCCC
VTAASIQDAQMAGLLDGRGATDADGPALPVGTTLPGPAWNPTAGVRQYQNQADWQGNGQA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
QLGQLPNDTPEHISSFVNRMAGPAMVASRASGVPAKLIVGQAALESGWGRREILNSDGST
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCC
SFNVFGIKAGANWKGPTTEVLTTEYVDGQPQKVRARFRAYGSYEEACNDYARLLTSNPRY
EEEEEEEECCCCCCCCCCHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
AGVVAANSADEAAHGLQRAGYATDPAYGEKLVRIMKKVST
EEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGATLSGQSDISQRFALDTQGFEALKNQARAGNNSGSLQAAARQFEAVFTNMVLKSMRD
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
ATPSDGLFDNEQTKLYMSMMDQQLSQQLSTRGIGLADVMIRQLARATGQQLPPNMATAGG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHCCC
VTAASIQDAQMAGLLDGRGATDADGPALPVGTTLPGPAWNPTAGVRQYQNQADWQGNGQA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
QLGQLPNDTPEHISSFVNRMAGPAMVASRASGVPAKLIVGQAALESGWGRREILNSDGST
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCC
SFNVFGIKAGANWKGPTTEVLTTEYVDGQPQKVRARFRAYGSYEEACNDYARLLTSNPRY
EEEEEEEECCCCCCCCCCHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
AGVVAANSADEAAHGLQRAGYATDPAYGEKLVRIMKKVST
EEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]