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The map label for this gene is pncA [H]

Identifier: 94310792

GI number: 94310792

Start: 2008700

End: 2009365

Strand: Reverse

Name: pncA [H]

Synonym: Rmet_1854

Alternate gene names: 94310792

Gene position: 2009365-2008700 (Counterclockwise)

Preceding gene: 94310793

Following gene: 94310791

Centisome position: 51.15

GC content: 66.82

Gene sequence:

>666_bases
ATGGCCTATGGCGCGCAGGATTGCCTGCTGGTGATCGATGTGCAGAACGACTTCATGCCGGGGGGCGCGCTGGCCGTGCC
GCGCGGCGATGAAGTCGTGCCGATCATCAACCGGTTGGCGCCGGCATTCGAGCACGTGGTGCTGACGCAGGACTGGCATC
CCGCCGGTCATGTGTCCTTCGCAACGAGCCATGTCGGTACCGCGCCGTTCCAGACCATGATGTTGCCGTATGGCGAGCAA
GTGCTGTGGCCCGATCACTGCGTGCAGGGCACGCCCGGCGCCGCGCTGCATGCAGACCTGCGCATCCCGCACGCGCGGAT
GGTGATCCGCAAGGGTCACCACGCCAACGTGGACAGCTACAGCGCGTTCATGGAGGCGGACCGCGCGACGAAGACCGGAC
TGGCTGGCTACCTGCGCGAGCAGGGCGTTCGCCGGGTGGTCTGCGTCGGGTTGGCAACGGATTACTGCGTGGCCTGGACC
GCGCTCGATGCGCGCGCAGCGGGCTTCGAGGCGGTGGTCGTGGAAGATGCCTGCCGCGCGATCGATCTGAACGGATCGCT
CGACCGGGCATGGCGAGAACTGGCTGCGGCCGGTGTTCGTCGTACGACTGCCGATGCACTGCGGTCTGGTGCTGGTACGC
ACAATCCCTGGCTCCCTGAGTCCTGA

Upstream 100 bases:

>100_bases
AGCGTGGGTGGTGGCGCCGCTGCTGCAGAAGCTGGCAGACGAGGGCAAGGGCTTCAACGGCTGATCGCGACCGCGTGAAC
GCAAAGGGATAACGCGAATC

Downstream 100 bases:

>100_bases
AGCGTCACGCAACGAAAGCCGAACACACCGAATATTTCTCGCAAGTTTGCAAGAGACACCAAGATACCGAGGAGCAACAC
ATGAACGAGGCAGTCATCGT

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAYGAQDCLLVIDVQNDFMPGGALAVPRGDEVVPIINRLAPAFEHVVLTQDWHPAGHVSFATSHVGTAPFQTMMLPYGEQ
VLWPDHCVQGTPGAALHADLRIPHARMVIRKGHHANVDSYSAFMEADRATKTGLAGYLREQGVRRVVCVGLATDYCVAWT
ALDARAAGFEAVVVEDACRAIDLNGSLDRAWRELAAAGVRRTTADALRSGAGTHNPWLPES

Sequences:

>Translated_221_residues
MAYGAQDCLLVIDVQNDFMPGGALAVPRGDEVVPIINRLAPAFEHVVLTQDWHPAGHVSFATSHVGTAPFQTMMLPYGEQ
VLWPDHCVQGTPGAALHADLRIPHARMVIRKGHHANVDSYSAFMEADRATKTGLAGYLREQGVRRVVCVGLATDYCVAWT
ALDARAAGFEAVVVEDACRAIDLNGSLDRAWRELAAAGVRRTTADALRSGAGTHNPWLPES
>Mature_220_residues
AYGAQDCLLVIDVQNDFMPGGALAVPRGDEVVPIINRLAPAFEHVVLTQDWHPAGHVSFATSHVGTAPFQTMMLPYGEQV
LWPDHCVQGTPGAALHADLRIPHARMVIRKGHHANVDSYSAFMEADRATKTGLAGYLREQGVRRVVCVGLATDYCVAWTA
LDARAAGFEAVVVEDACRAIDLNGSLDRAWRELAAAGVRRTTADALRSGAGTHNPWLPES

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=200, Percent_Identity=44, Blast_Score=160, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI193206735, Length=236, Percent_Identity=31.3559322033898, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17543130, Length=236, Percent_Identity=30.9322033898305, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71999339, Length=215, Percent_Identity=32.5581395348837, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=210, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648247, Length=199, Percent_Identity=35.678391959799, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23819; Mature: 23688

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYGAQDCLLVIDVQNDFMPGGALAVPRGDEVVPIINRLAPAFEHVVLTQDWHPAGHVSF
CCCCCCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEE
ATSHVGTAPFQTMMLPYGEQVLWPDHCVQGTPGAALHADLRIPHARMVIRKGHHANVDSY
ECCCCCCCCHHHHHCCCCCEEECCHHHCCCCCCCEEEEECCCCHHHHHEECCCCCCCHHH
SAFMEADRATKTGLAGYLREQGVRRVVCVGLATDYCVAWTALDARAAGFEAVVVEDACRA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCHHE
IDLNGSLDRAWRELAAAGVRRTTADALRSGAGTHNPWLPES
EECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AYGAQDCLLVIDVQNDFMPGGALAVPRGDEVVPIINRLAPAFEHVVLTQDWHPAGHVSF
CCCCCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEE
ATSHVGTAPFQTMMLPYGEQVLWPDHCVQGTPGAALHADLRIPHARMVIRKGHHANVDSY
ECCCCCCCCHHHHHCCCCCEEECCHHHCCCCCCCEEEEECCCCHHHHHEECCCCCCCHHH
SAFMEADRATKTGLAGYLREQGVRRVVCVGLATDYCVAWTALDARAAGFEAVVVEDACRA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEECCHHE
IDLNGSLDRAWRELAAAGVRRTTADALRSGAGTHNPWLPES
EECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]