BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is ligD [H]

Identifier: 94310784

GI number: 94310784

Start: 2000070

End: 2000972

Strand: Reverse

Name: ligD [H]

Synonym: Rmet_1846

Alternate gene names: 94310784

Gene position: 2000972-2000070 (Counterclockwise)

Preceding gene: 94310785

Following gene: 94310783

Centisome position: 50.94

GC content: 63.68

Gene sequence:

>903_bases
ATGAAGCAGTTCGAGAGCAAGGTGGCAGTGATTACCGGCGGTGCTTCAGGATTCGGCAAGGAATTCGCGCGAATCGGTGC
GTCGCTGGGCATGAAGCTGGTGCTGGCGGACGTCCAGGAGGACTCGCTGGACAGCACCGTGGCCGAATTCAAATCGCAGG
GCGCCCAGGTGATCGGGTTGCGCACGGACGTGTCGAAGGCCGAGGAAGTCCAGGTGCTGGCCGATGCCGCGATGACGGCG
TTCGGCGAGGTCAACCTGCTGTTCAACAACGCGGGTGTGGGCGCGGGCGGCCTGATCTGGGAAAACACGCAGAAAGACTG
GGACTGGGTGCTCGGCGTGAACCTCTACGGCGTGGTCAATGGCGTGCGGATCTTCACGCCGCTGATGCTGGAGGCGGCCC
GCAAGGACCCGGGCTATCAGGGGCATATCGTCAATACCGCGTCGATGGCGGGCCTGCTGAATCCGCCGGCCATGGGCGTG
TACAACGTTTCCAAACATGCGGTGGTCGCGTTGTCCGAGACGCTCTACCAGGATCTGAGCCTGGTCACGGACCAGGTGCA
TTGCTCGGTGCTGTGCCCGTTCTACGTGCCCACCGGCATCAGCCAGTCGCAGCGTAACCGGCCGGCCAATATGGCCAACG
ATCAACCACCCACGCGCTCGCAGATGGTGTCGCAGGCGCTGTCCGACAAGGCGGTCAACTCGGGCAAGGTTAGCGCGGCC
GACGTGGCGCAGATGACGTTCGATGCGATCCGCGAGGACGGTTTCTATATCTACTCCCACCCGCAATCGCTGGATTCCGT
CCAGCGGCGCATGGAGGAGATCGTCAACCAGCGCAATCCGGGCGATCCGTTCGGTGACAAGAGCGAAGTGCGGGCGGGCC
TGATCGCCGCGCTGCGCGGCTAA

Upstream 100 bases:

>100_bases
CATTACCAGGTGCAAGCGGCATAGGCATAGGCATAGCCCATCCAGACAAGAACTACGAACACTACGTTCACTACGAACGA
CAAGTAGAGGGAGACGTTTC

Downstream 100 bases:

>100_bases
GCTGGCCAGGAACTTGCGTACACGAACACGAACAGGACGAACCGGACAACCGATATGGCCGAGACCGCCAGGGAGACCAT
GATGACCGCACCCGCCGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKQFESKVAVITGGASGFGKEFARIGASLGMKLVLADVQEDSLDSTVAEFKSQGAQVIGLRTDVSKAEEVQVLADAAMTA
FGEVNLLFNNAGVGAGGLIWENTQKDWDWVLGVNLYGVVNGVRIFTPLMLEAARKDPGYQGHIVNTASMAGLLNPPAMGV
YNVSKHAVVALSETLYQDLSLVTDQVHCSVLCPFYVPTGISQSQRNRPANMANDQPPTRSQMVSQALSDKAVNSGKVSAA
DVAQMTFDAIREDGFYIYSHPQSLDSVQRRMEEIVNQRNPGDPFGDKSEVRAGLIAALRG

Sequences:

>Translated_300_residues
MKQFESKVAVITGGASGFGKEFARIGASLGMKLVLADVQEDSLDSTVAEFKSQGAQVIGLRTDVSKAEEVQVLADAAMTA
FGEVNLLFNNAGVGAGGLIWENTQKDWDWVLGVNLYGVVNGVRIFTPLMLEAARKDPGYQGHIVNTASMAGLLNPPAMGV
YNVSKHAVVALSETLYQDLSLVTDQVHCSVLCPFYVPTGISQSQRNRPANMANDQPPTRSQMVSQALSDKAVNSGKVSAA
DVAQMTFDAIREDGFYIYSHPQSLDSVQRRMEEIVNQRNPGDPFGDKSEVRAGLIAALRG
>Mature_300_residues
MKQFESKVAVITGGASGFGKEFARIGASLGMKLVLADVQEDSLDSTVAEFKSQGAQVIGLRTDVSKAEEVQVLADAAMTA
FGEVNLLFNNAGVGAGGLIWENTQKDWDWVLGVNLYGVVNGVRIFTPLMLEAARKDPGYQGHIVNTASMAGLLNPPAMGV
YNVSKHAVVALSETLYQDLSLVTDQVHCSVLCPFYVPTGISQSQRNRPANMANDQPPTRSQMVSQALSDKAVNSGKVSAA
DVAQMTFDAIREDGFYIYSHPQSLDSVQRRMEEIVNQRNPGDPFGDKSEVRAGLIAALRG

Specific function: Catalyzes the C alpha dehydrogenation of arylglycerol- beta-aryl ether (C alpha alcohol type) (compound IV) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=200, Percent_Identity=31.5, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31542939, Length=204, Percent_Identity=29.9019607843137, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI20149619, Length=204, Percent_Identity=28.921568627451, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI25282469, Length=253, Percent_Identity=28.0632411067194, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI33667109, Length=203, Percent_Identity=30.0492610837438, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI5031737, Length=200, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI32483357, Length=200, Percent_Identity=30, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI40807363, Length=230, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI109715829, Length=202, Percent_Identity=27.2277227722772, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310128830, Length=215, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310115468, Length=215, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI224922801, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4758504, Length=227, Percent_Identity=27.7533039647577, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI210032110, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI83715985, Length=185, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI87082100, Length=204, Percent_Identity=28.921568627451, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787905, Length=148, Percent_Identity=31.0810810810811, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789208, Length=222, Percent_Identity=27.4774774774775, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=206, Percent_Identity=27.1844660194175, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787335, Length=201, Percent_Identity=22.8855721393035, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789057, Length=188, Percent_Identity=27.1276595744681, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=199, Percent_Identity=30.6532663316583, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193203117, Length=194, Percent_Identity=30.9278350515464, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564282, Length=200, Percent_Identity=29, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17568967, Length=309, Percent_Identity=27.5080906148867, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17508895, Length=200, Percent_Identity=27, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=203, Percent_Identity=26.6009852216749, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=234, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=209, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=202, Percent_Identity=24.2574257425743, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=184, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319635, Length=206, Percent_Identity=25.7281553398058, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640492, Length=207, Percent_Identity=29.4685990338164, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355319, Length=214, Percent_Identity=29.9065420560748, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581726, Length=217, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364892, Length=229, Percent_Identity=27.5109170305677, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571526, Length=181, Percent_Identity=30.3867403314917, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221510726, Length=196, Percent_Identity=30.1020408163265, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21355631, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639444, Length=193, Percent_Identity=28.4974093264249, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357041, Length=181, Percent_Identity=27.6243093922652, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281364494, Length=232, Percent_Identity=28.8793103448276, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24644339, Length=190, Percent_Identity=28.4210526315789, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18859837, Length=267, Percent_Identity=25.0936329588015, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737361, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007236, Length=206, Percent_Identity=28.1553398058252, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007238, Length=198, Percent_Identity=28.2828282828283, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641388, Length=238, Percent_Identity=30.672268907563, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571387, Length=204, Percent_Identity=26.9607843137255, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI23397609, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 32200; Mature: 32200

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFESKVAVITGGASGFGKEFARIGASLGMKLVLADVQEDSLDSTVAEFKSQGAQVIGL
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEE
RTDVSKAEEVQVLADAAMTAFGEVNLLFNNAGVGAGGLIWENTQKDWDWVLGVNLYGVVN
ECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCEEECCHHHHHHH
GVRIFTPLMLEAARKDPGYQGHIVNTASMAGLLNPPAMGVYNVSKHAVVALSETLYQDLS
HHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
LVTDQVHCSVLCPFYVPTGISQSQRNRPANMANDQPPTRSQMVSQALSDKAVNSGKVSAA
HHHHHHHEEEEECCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
DVAQMTFDAIREDGFYIYSHPQSLDSVQRRMEEIVNQRNPGDPFGDKSEVRAGLIAALRG
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQFESKVAVITGGASGFGKEFARIGASLGMKLVLADVQEDSLDSTVAEFKSQGAQVIGL
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEE
RTDVSKAEEVQVLADAAMTAFGEVNLLFNNAGVGAGGLIWENTQKDWDWVLGVNLYGVVN
ECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCEEECCHHHHHHH
GVRIFTPLMLEAARKDPGYQGHIVNTASMAGLLNPPAMGVYNVSKHAVVALSETLYQDLS
HHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
LVTDQVHCSVLCPFYVPTGISQSQRNRPANMANDQPPTRSQMVSQALSDKAVNSGKVSAA
HHHHHHHEEEEECCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
DVAQMTFDAIREDGFYIYSHPQSLDSVQRRMEEIVNQRNPGDPFGDKSEVRAGLIAALRG
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726; 7764263 [H]