Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 93007070

GI number: 93007070

Start: 2755114

End: 2756586

Strand: Direct

Name: mltB [H]

Synonym: Pcryo_2246

Alternate gene names: 93007070

Gene position: 2755114-2756586 (Clockwise)

Preceding gene: 93007069

Following gene: 93007075

Centisome position: 90.04

GC content: 46.03

Gene sequence:

>1473_bases
ATGCGCTTGAATCACTTATCCACCTTATCTTTATTGACGGCAGCGATTGGCTTAAGTCTTGCCAGCACCGCTCAAGCTGC
CAAGCCAGACGCCCCAAACTTGTCTAATGCTCAGTTTCAGCAGTGTCTAGATGGTCTAAAAAACTCTAGCAAATTTCGTG
ATGTTGATAGCTATACCTTTAATAACTATCGTCCCAGTGAGCCTGACCCTAGTGTTATTCAATCGCTAAACTATCAACCT
GAATTTCAAAAAGACGTTTGGGATTATTTGTCATCACTTGTTGATACCGAACGCGTAGAAGATGGTATCCGTGCCAAGCG
TCAATGGGCGGATACGTTACGTAAAATTGAATCGCGCTATGGCGTTAAAGCCGAGCACGTATTAGGCGTTTGGGGCGTAG
AGTCTAACTTTGGGCAGACATTGGGCAAAAAACCTTTGTTTGAATCTTTAGCGACCCTATCTTGCTTTGATCGCCGCCAA
AGCTATTTCCAAGGCGAATATGCCAATGCACTAAAAATCGTCCAAAACGGTGATATTGCGCCAAGCGATATGACAGGTTC
ATGGGCAGGCGCGTTTGGTCAGACGCAGTTTATGCCAAGTACATTTTTAGAGTTGGCAGTCGACTTCGATGGCGATGGTC
GCCGCGACTTGGTCAATAGCGTCCCCGATGCCCTTGCCTCTACGGCAAACTTCTTAGATAAACGTGGCTACCGTTCAGGC
GAAGCATGGGGTTATGAGGTAAAACTGCCAAGTGGATTTTGGGCAGCGTCCAACCGTAAAGATAAAAAATCGATGAGCCA
CTGGCGCAATCAAGGTTTAACCCTTGCTAATGGCAGCCCGCTTCCTTCTGACTTGAGTAGTGCTGGATTATTATTACCAG
CCGGTCCTGACGGCCCTGCATTCTTAGTCGGTAAAAACTTTGATACGTTCTATTCTTATAACGCCTCAGAAAGCTATGCA
CTGGCGATTGCCCATTTATCAGATTTGATTACTCGCGAGAGTAGTAGTAAAACTGACTTTATCACAGCATGGCCAACTGA
CGATCCTGGTATTGGTCGTCAAGAATCCAGAGCGATTCAGCAAGCATTATTAAATGCAGGATATGATATCGGCGCCGTCG
ATGGCATTATCGGTGATAATACTCGTACTGCTATCCAGCAGTACCAGTCTAGCCGTGGCATTTTTCCAGCTGATGGACGT
GCAGGGCAGAAATTCTATCGTGCCATCGTTGGTAATACGGCGCCTAATACTTCGCAATACGGCAATCAGTATAACCAGCC
AACAAACAGTCGTCCTTTAAACCCCGCGTCTGCTGATCGTATGGGACAATTAATTCAACAGCAAAATACTACATCTAATA
GCTACTCACAGCCTATAGCGATCAGCCCATCAAAATCAAGCACAACGCGATATCGCCGTGTAACGGGTGCTGATGGTACT
ATAAAACTGGTACGTATTGATGAAGGCTCTTGA

Upstream 100 bases:

>100_bases
CTAAGTAAAATCGACTTTTCATCGCTTTGTTTTACTTTCTAATAACTAATTTCTAGTAACTGTTTTTTAACAGCTACTTC
GACGCTTATAGGATATTTTT

Downstream 100 bases:

>100_bases
TTAGGTAATGCCTTTTAAGTATTAATGAAAATAAAAAGCCACGCTAATTAGCGTGGCTTTTTGATCATGTCAGTTCTGAC
ACTCTTTATGACTTGTCACA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRLNHLSTLSLLTAAIGLSLASTAQAAKPDAPNLSNAQFQQCLDGLKNSSKFRDVDSYTFNNYRPSEPDPSVIQSLNYQP
EFQKDVWDYLSSLVDTERVEDGIRAKRQWADTLRKIESRYGVKAEHVLGVWGVESNFGQTLGKKPLFESLATLSCFDRRQ
SYFQGEYANALKIVQNGDIAPSDMTGSWAGAFGQTQFMPSTFLELAVDFDGDGRRDLVNSVPDALASTANFLDKRGYRSG
EAWGYEVKLPSGFWAASNRKDKKSMSHWRNQGLTLANGSPLPSDLSSAGLLLPAGPDGPAFLVGKNFDTFYSYNASESYA
LAIAHLSDLITRESSSKTDFITAWPTDDPGIGRQESRAIQQALLNAGYDIGAVDGIIGDNTRTAIQQYQSSRGIFPADGR
AGQKFYRAIVGNTAPNTSQYGNQYNQPTNSRPLNPASADRMGQLIQQQNTTSNSYSQPIAISPSKSSTTRYRRVTGADGT
IKLVRIDEGS

Sequences:

>Translated_490_residues
MRLNHLSTLSLLTAAIGLSLASTAQAAKPDAPNLSNAQFQQCLDGLKNSSKFRDVDSYTFNNYRPSEPDPSVIQSLNYQP
EFQKDVWDYLSSLVDTERVEDGIRAKRQWADTLRKIESRYGVKAEHVLGVWGVESNFGQTLGKKPLFESLATLSCFDRRQ
SYFQGEYANALKIVQNGDIAPSDMTGSWAGAFGQTQFMPSTFLELAVDFDGDGRRDLVNSVPDALASTANFLDKRGYRSG
EAWGYEVKLPSGFWAASNRKDKKSMSHWRNQGLTLANGSPLPSDLSSAGLLLPAGPDGPAFLVGKNFDTFYSYNASESYA
LAIAHLSDLITRESSSKTDFITAWPTDDPGIGRQESRAIQQALLNAGYDIGAVDGIIGDNTRTAIQQYQSSRGIFPADGR
AGQKFYRAIVGNTAPNTSQYGNQYNQPTNSRPLNPASADRMGQLIQQQNTTSNSYSQPIAISPSKSSTTRYRRVTGADGT
IKLVRIDEGS
>Mature_490_residues
MRLNHLSTLSLLTAAIGLSLASTAQAAKPDAPNLSNAQFQQCLDGLKNSSKFRDVDSYTFNNYRPSEPDPSVIQSLNYQP
EFQKDVWDYLSSLVDTERVEDGIRAKRQWADTLRKIESRYGVKAEHVLGVWGVESNFGQTLGKKPLFESLATLSCFDRRQ
SYFQGEYANALKIVQNGDIAPSDMTGSWAGAFGQTQFMPSTFLELAVDFDGDGRRDLVNSVPDALASTANFLDKRGYRSG
EAWGYEVKLPSGFWAASNRKDKKSMSHWRNQGLTLANGSPLPSDLSSAGLLLPAGPDGPAFLVGKNFDTFYSYNASESYA
LAIAHLSDLITRESSSKTDFITAWPTDDPGIGRQESRAIQQALLNAGYDIGAVDGIIGDNTRTAIQQYQSSRGIFPADGR
AGQKFYRAIVGNTAPNTSQYGNQYNQPTNSRPLNPASADRMGQLIQQQNTTSNSYSQPIAISPSKSSTTRYRRVTGADGT
IKLVRIDEGS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=248, Percent_Identity=34.2741935483871, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 53612; Mature: 53612

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNHLSTLSLLTAAIGLSLASTAQAAKPDAPNLSNAQFQQCLDGLKNSSKFRDVDSYTF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
NNYRPSEPDPSVIQSLNYQPEFQKDVWDYLSSLVDTERVEDGIRAKRQWADTLRKIESRY
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVKAEHVLGVWGVESNFGQTLGKKPLFESLATLSCFDRRQSYFQGEYANALKIVQNGDIA
CCCHHHEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCC
PSDMTGSWAGAFGQTQFMPSTFLELAVDFDGDGRRDLVNSVPDALASTANFLDKRGYRSG
CCCCCCCHHCCCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAWGYEVKLPSGFWAASNRKDKKSMSHWRNQGLTLANGSPLPSDLSSAGLLLPAGPDGPA
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHCCEEEECCCCCCE
FLVGKNFDTFYSYNASESYALAIAHLSDLITRESSSKTDFITAWPTDDPGIGRQESRAIQ
EEEECCCHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHH
QALLNAGYDIGAVDGIIGDNTRTAIQQYQSSRGIFPADGRAGQKFYRAIVGNTAPNTSQY
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
GNQYNQPTNSRPLNPASADRMGQLIQQQNTTSNSYSQPIAISPSKSSTTRYRRVTGADGT
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHEEEECCCCE
IKLVRIDEGS
EEEEEECCCC
>Mature Secondary Structure
MRLNHLSTLSLLTAAIGLSLASTAQAAKPDAPNLSNAQFQQCLDGLKNSSKFRDVDSYTF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
NNYRPSEPDPSVIQSLNYQPEFQKDVWDYLSSLVDTERVEDGIRAKRQWADTLRKIESRY
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVKAEHVLGVWGVESNFGQTLGKKPLFESLATLSCFDRRQSYFQGEYANALKIVQNGDIA
CCCHHHEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCC
PSDMTGSWAGAFGQTQFMPSTFLELAVDFDGDGRRDLVNSVPDALASTANFLDKRGYRSG
CCCCCCCHHCCCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAWGYEVKLPSGFWAASNRKDKKSMSHWRNQGLTLANGSPLPSDLSSAGLLLPAGPDGPA
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHCCEEEECCCCCCE
FLVGKNFDTFYSYNASESYALAIAHLSDLITRESSSKTDFITAWPTDDPGIGRQESRAIQ
EEEECCCHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHH
QALLNAGYDIGAVDGIIGDNTRTAIQQYQSSRGIFPADGRAGQKFYRAIVGNTAPNTSQY
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
GNQYNQPTNSRPLNPASADRMGQLIQQQNTTSNSYSQPIAISPSKSSTTRYRRVTGADGT
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHEEEECCCCE
IKLVRIDEGS
EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]