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The map label for this gene is rph [H]

Identifier: 93006978

GI number: 93006978

Start: 2636883

End: 2637599

Strand: Reverse

Name: rph [H]

Synonym: Pcryo_2154

Alternate gene names: 93006978

Gene position: 2637599-2636883 (Counterclockwise)

Preceding gene: 93006979

Following gene: 93006977

Centisome position: 86.2

GC content: 46.72

Gene sequence:

>717_bases
ATGCGTATTGACAACCGTGAGCTTAATCAACTTCGCTCAATCAGCTTTGAGCGCCATTACACTAAGCATGCTGAAGGGTC
AGTATTGGTCAGCTTTGGCGACACCAAAGTACTGTGCACCGCAAGTGTAGAGTCTGGCGTACCGCGTTGGCTCAAAGGTA
AAGGCAAAGGCTGGATCACGGCTGAATATGGCATGTTGCCACGCGCGACCAATACTCGTAATCAACGTGAAGCGGCTCGC
GGTAAGCAATCAGGTCGTACTCAAGAAATTCAGCGTTTAATCGGTCGTAGTTTACGTGCCATGATTGATTTGAGTAAGCT
TGGCGAAAATACCATTTACTTAGATTGTGATGTCTTGCAAGCGGATGGTGGTACTCGTACTGCTAGTGTGACCGGTGCCG
CGATTGCCCTTATCGATGCCTTAGAGAGCATCCAAAAAACTAAGAAACTAAAAGCGGATCCATTGATTGGTTTGGTTGCT
GCCGTTTCTGTTGGTATGAAAGATGGTAAAGCGTACTTGGATTTAAACTACGAAGAAGATGCCAGCTGTGATACGGATTT
GAACGTGGTGATGACCCAAAAAGGCGAGTTCATTGAGCTACAAGGGACGGCGGAAGAAAAGCCATTTACTCGTGCTCAAG
CTGACGATATGCTTATTCTAGCCGAAAAAGGTATCGCTGAGCTGATTGCTATGCAAAAAACCGCCTTAGGTTGGTAG

Upstream 100 bases:

>100_bases
TTTGCTGGAAATACTGATTGGCTAACCACATAACCATAAATTTAAATCTAAACCTCTTATTTTATAACACTGCTTTTACA
ACTTCGTACTAGGGAATTTT

Downstream 100 bases:

>100_bases
TCATATAAGACCGCAATACGGCTTACTATTGATATTTTTATAATACTTTCACTGCTTTTCTATAGGTAAACGTGCATGCT
TAAGCTGACTGATGACGGCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRIDNRELNQLRSISFERHYTKHAEGSVLVSFGDTKVLCTASVESGVPRWLKGKGKGWITAEYGMLPRATNTRNQREAAR
GKQSGRTQEIQRLIGRSLRAMIDLSKLGENTIYLDCDVLQADGGTRTASVTGAAIALIDALESIQKTKKLKADPLIGLVA
AVSVGMKDGKAYLDLNYEEDASCDTDLNVVMTQKGEFIELQGTAEEKPFTRAQADDMLILAEKGIAELIAMQKTALGW

Sequences:

>Translated_238_residues
MRIDNRELNQLRSISFERHYTKHAEGSVLVSFGDTKVLCTASVESGVPRWLKGKGKGWITAEYGMLPRATNTRNQREAAR
GKQSGRTQEIQRLIGRSLRAMIDLSKLGENTIYLDCDVLQADGGTRTASVTGAAIALIDALESIQKTKKLKADPLIGLVA
AVSVGMKDGKAYLDLNYEEDASCDTDLNVVMTQKGEFIELQGTAEEKPFTRAQADDMLILAEKGIAELIAMQKTALGW
>Mature_238_residues
MRIDNRELNQLRSISFERHYTKHAEGSVLVSFGDTKVLCTASVESGVPRWLKGKGKGWITAEYGMLPRATNTRNQREAAR
GKQSGRTQEIQRLIGRSLRAMIDLSKLGENTIYLDCDVLQADGGTRTASVTGAAIALIDALESIQKTKKLKADPLIGLVA
AVSVGMKDGKAYLDLNYEEDASCDTDLNVVMTQKGEFIELQGTAEEKPFTRAQADDMLILAEKGIAELIAMQKTALGW

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=67.2985781990521, Blast_Score=302, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI71981632, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26065; Mature: 26065

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDNRELNQLRSISFERHYTKHAEGSVLVSFGDTKVLCTASVESGVPRWLKGKGKGWIT
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECHHHCCCHHHCCCCCEEEE
AEYGMLPRATNTRNQREAARGKQSGRTQEIQRLIGRSLRAMIDLSKLGENTIYLDCDVLQ
ECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASVTGAAIALIDALESIQKTKKLKADPLIGLVAAVSVGMKDGKAYLDLNYEED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
ASCDTDLNVVMTQKGEFIELQGTAEEKPFTRAQADDMLILAEKGIAELIAMQKTALGW
CCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIDNRELNQLRSISFERHYTKHAEGSVLVSFGDTKVLCTASVESGVPRWLKGKGKGWIT
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECHHHCCCHHHCCCCCEEEE
AEYGMLPRATNTRNQREAARGKQSGRTQEIQRLIGRSLRAMIDLSKLGENTIYLDCDVLQ
ECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASVTGAAIALIDALESIQKTKKLKADPLIGLVAAVSVGMKDGKAYLDLNYEED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
ASCDTDLNVVMTQKGEFIELQGTAEEKPFTRAQADDMLILAEKGIAELIAMQKTALGW
CCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA