BacMap

Definition	Psychrobacter cryohalolentis K5 chromosome, complete genome.
Accession	NC_007969
Length	3,059,876

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The map label for this gene is dkgA [H]

Identifier: 93006932

GI number: 93006932

Start: 2582960

End: 2583808

Strand: Direct

Name: dkgA [H]

Synonym: Pcryo_2108

Alternate gene names: 93006932

Gene position: 2582960-2583808 (Clockwise)

Preceding gene: 93006931

Following gene: 93006938

Centisome position: 84.41

GC content: 43.82

Gene sequence:

>849_bases
ATGCGTGCTAAAACTTATAACATTCGCACCGCTGGACAAGCGAACATTCCGGTACTCGGACTGGGTACGTGGCAATCAAC
GGGACAAGACTGTATTGATGTCGTCAGCCAAGGTCTAAGAATGGGCTATGAACACATTGATACGGCTCAAGCATATGGCA
ATGAAGTCGAGGTTGGTAAAGGTATTAAGCAATCAGGTGTCGCTCGCGATAAGTTCTTTTTAACGACCAAAATATTCCCT
GATGACATGAAGTTTGAGCCAGAGAAACTCGTTGCCGCTGCTAAGCGCTCATTGGAAAATCTAGATACCGATTACGTTGA
TTTACTGCTGCTACATTGGCCTGATGATCGCGTGCCCTTATCTGAAACCATCCCCGCGCTTTGTGAGCTCCAAAAACAAG
GTTTGACTCGCAATATTGGTGTGTCTAACTTTAATATTGCCAATATCATTGAAGCCAAACGATATGCTGACGTGCCGATT
GTAGTGAACCAAGTCGAGTTTCATCCTTTTATTAAGCAAAAAACGTTGCAGACCTTTTTAAATAATCACCATATTTTATT
GGAAGCGTATTCGCCGCTTGCCCGCGGAGATGTTTTTGATAATAAAATCATTAAAGACATCGCTGATAAACACGGTATCA
CCGCCGCACAAGTGTCGCTGGCGTGGATTCTATCCGATAAGCACCGTATTGCGATTCCAAAAACGTCTAATCCTGAGCAC
TTACAAGGCAACTTAGATGCCATCAAAGTAGAGTTGAGTGCGGATGAACTTGAGAAAATCGGCAGCTTAGCGCGTAGTGA
CGGGCGTAAAATTAAGCATCCAGATTATTCGCCAGAATGGGATGATTAA

Upstream 100 bases:

>100_bases
AAGGCTTAATAGAGATTGAGAACATTATATTTCTGATCGAATATTTTATTAATAACTATAAAAGCTATTAGTAGATCACA
AAAAAGACAAGGATAATAAT

Downstream 100 bases:

>100_bases
TAAATAACTCATACTTATTTAAGTATGAGGTTTGCCAAAAAAAATCCAGTGATAAAGGTCACTGGATTTTTTTATGTTTA
ATGCTTTTTACGATAATTAT

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRAKTYNIRTAGQANIPVLGLGTWQSTGQDCIDVVSQGLRMGYEHIDTAQAYGNEVEVGKGIKQSGVARDKFFLTTKIFP
DDMKFEPEKLVAAAKRSLENLDTDYVDLLLLHWPDDRVPLSETIPALCELQKQGLTRNIGVSNFNIANIIEAKRYADVPI
VVNQVEFHPFIKQKTLQTFLNNHHILLEAYSPLARGDVFDNKIIKDIADKHGITAAQVSLAWILSDKHRIAIPKTSNPEH
LQGNLDAIKVELSADELEKIGSLARSDGRKIKHPDYSPEWDD

Sequences:

>Translated_282_residues
MRAKTYNIRTAGQANIPVLGLGTWQSTGQDCIDVVSQGLRMGYEHIDTAQAYGNEVEVGKGIKQSGVARDKFFLTTKIFP
DDMKFEPEKLVAAAKRSLENLDTDYVDLLLLHWPDDRVPLSETIPALCELQKQGLTRNIGVSNFNIANIIEAKRYADVPI
VVNQVEFHPFIKQKTLQTFLNNHHILLEAYSPLARGDVFDNKIIKDIADKHGITAAQVSLAWILSDKHRIAIPKTSNPEH
LQGNLDAIKVELSADELEKIGSLARSDGRKIKHPDYSPEWDD
>Mature_282_residues
MRAKTYNIRTAGQANIPVLGLGTWQSTGQDCIDVVSQGLRMGYEHIDTAQAYGNEVEVGKGIKQSGVARDKFFLTTKIFP
DDMKFEPEKLVAAAKRSLENLDTDYVDLLLLHWPDDRVPLSETIPALCELQKQGLTRNIGVSNFNIANIIEAKRYADVPI
VVNQVEFHPFIKQKTLQTFLNNHHILLEAYSPLARGDVFDNKIIKDIADKHGITAAQVSLAWILSDKHRIAIPKTSNPEH
LQGNLDAIKVELSADELEKIGSLARSDGRKIKHPDYSPEWDD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=309, Percent_Identity=30.0970873786408, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI5174391, Length=309, Percent_Identity=30.0970873786408, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI223468663, Length=295, Percent_Identity=31.1864406779661, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=31.4754098360656, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI45446745, Length=290, Percent_Identity=30, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI4503285, Length=290, Percent_Identity=30, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI310109922, Length=271, Percent_Identity=29.8892988929889, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI310109920, Length=290, Percent_Identity=29.6551724137931, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI5453543, Length=290, Percent_Identity=29.6551724137931, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=31.8339100346021, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI24497585, Length=305, Percent_Identity=28.1967213114754, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=30.5147058823529, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI5174695, Length=307, Percent_Identity=28.6644951140065, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI24497583, Length=305, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI300116273, Length=290, Percent_Identity=28.9655172413793, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI27436969, Length=316, Percent_Identity=27.5316455696203, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4504825, Length=316, Percent_Identity=27.5316455696203, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI27436966, Length=301, Percent_Identity=27.906976744186, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI27436962, Length=309, Percent_Identity=28.1553398058252, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI27436964, Length=301, Percent_Identity=27.906976744186, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI300116271, Length=285, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI27436971, Length=307, Percent_Identity=26.0586319218241, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI207028673, Length=106, Percent_Identity=36.7924528301887, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI310109926, Length=106, Percent_Identity=35.8490566037736, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87082198, Length=266, Percent_Identity=40.9774436090226, Blast_Score=190, Evalue=7e-50,
Organism=Escherichia coli, GI1786400, Length=269, Percent_Identity=38.6617100371747, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1788081, Length=270, Percent_Identity=34.0740740740741, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788070, Length=298, Percent_Identity=25.8389261744966, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787674, Length=287, Percent_Identity=27.8745644599303, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994888, Length=262, Percent_Identity=24.4274809160305, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789375, Length=304, Percent_Identity=21.7105263157895, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538386, Length=264, Percent_Identity=34.469696969697, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=30.2919708029197, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17561298, Length=267, Percent_Identity=31.8352059925094, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71998625, Length=268, Percent_Identity=30.9701492537313, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=32.7586206896552, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=31.7518248175182, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=32.4137931034483, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17562292, Length=270, Percent_Identity=32.5925925925926, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=31.3793103448276, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537079, Length=287, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17550248, Length=296, Percent_Identity=30.0675675675676, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17564128, Length=290, Percent_Identity=28.2758620689655, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320576, Length=301, Percent_Identity=35.2159468438538, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324694, Length=297, Percent_Identity=34.6801346801347, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321896, Length=297, Percent_Identity=30.3030303030303, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322556, Length=278, Percent_Identity=31.294964028777, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320079, Length=274, Percent_Identity=30.2919708029197, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319625, Length=288, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644950, Length=289, Percent_Identity=34.9480968858132, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI221378297, Length=309, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24657054, Length=301, Percent_Identity=32.2259136212625, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24663317, Length=274, Percent_Identity=33.5766423357664, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI20129731, Length=278, Percent_Identity=33.0935251798561, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24662789, Length=301, Percent_Identity=31.2292358803987, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=30.6896551724138, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45553081, Length=287, Percent_Identity=31.0104529616725, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=31.7857142857143, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24662785, Length=271, Percent_Identity=32.8413284132841, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281366140, Length=262, Percent_Identity=30.5343511450382, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24640980, Length=192, Percent_Identity=23.9583333333333, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45549126, Length=192, Percent_Identity=23.9583333333333, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 31564; Mature: 31564

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAKTYNIRTAGQANIPVLGLGTWQSTGQDCIDVVSQGLRMGYEHIDTAQAYGNEVEVGK
CCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCEECCCC
GIKQSGVARDKFFLTTKIFPDDMKFEPEKLVAAAKRSLENLDTDYVDLLLLHWPDDRVPL
CCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCH
SETIPALCELQKQGLTRNIGVSNFNIANIIEAKRYADVPIVVNQVEFHPFIKQKTLQTFL
HHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
NNHHILLEAYSPLARGDVFDNKIIKDIADKHGITAAQVSLAWILSDKHRIAIPKTSNPEH
CCCEEEEEECCCHHCCCHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECCCCCCHHH
LQGNLDAIKVELSADELEKIGSLARSDGRKIKHPDYSPEWDD
HCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAKTYNIRTAGQANIPVLGLGTWQSTGQDCIDVVSQGLRMGYEHIDTAQAYGNEVEVGK
CCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCEECCCC
GIKQSGVARDKFFLTTKIFPDDMKFEPEKLVAAAKRSLENLDTDYVDLLLLHWPDDRVPL
CCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCH
SETIPALCELQKQGLTRNIGVSNFNIANIIEAKRYADVPIVVNQVEFHPFIKQKTLQTFL
HHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
NNHHILLEAYSPLARGDVFDNKIIKDIADKHGITAAQVSLAWILSDKHRIAIPKTSNPEH
CCCEEEEEECCCHHCCCHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECCCCCCHHH
LQGNLDAIKVELSADELEKIGSLARSDGRKIKHPDYSPEWDD
HCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]