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The map label for this gene is nadR [H]

Identifier: 93006882

GI number: 93006882

Start: 2527086

End: 2528132

Strand: Direct

Name: nadR [H]

Synonym: Pcryo_2058

Alternate gene names: 93006882

Gene position: 2527086-2528132 (Clockwise)

Preceding gene: 93006877

Following gene: 93006883

Centisome position: 82.59

GC content: 45.56

Gene sequence:

>1047_bases
ATGTACGAGTCAGGATTGATGATTGGACATTTTGAGCCGCTACATTTGGGGCAAATGCGCAGTATATTGAACGCAGCAGG
ACAAGCAAAAAACTTACATCTTGTCATTATGACCCACCCGTCGCCGCATCCAGATTTTAAAATCACGTTGCAAGATAAAG
CGCGTTGGTTACAAATGGCTTGTGCTGATTTACCTTTTATTCATATTCATACCACTGATGAGATTGAGCTACCAGTACAT
GAAAGCTTTGCAGATGTGACGATTGATATCACTGCCAGTAACGCTAAGCTGCAACATTTGATAGATGCGTTAAACTTGCC
TGCTGAAACCGTAGTATTTGTCGCAGAGAACCACCCTTTAGCACAGTCTGATATTTGTAAGCAGCTAATCATGCAGGTGG
TCACAACACCGTTACAGCCTGACTTTGACTCTTATGCCATTGCACGAAATCCGGTCGCATATTGGTCAGCGCTACATCCG
CAAGCACGCAGTAACTATACTAAGACGGTTGCTATTGTCGGCGGCGAAAGCTCAGGTAAGACGACCTTGGTGCATAAGCT
GGCCAATTATTATGGTGCTAGCTTTGCGCTTGAGATGGGACGGGTTTATGTCGGTACAGATTTGGGCGGCACCGAAATTG
GACTACAATATAGCGATTATGGACCGATTGCCTTGCACCATGCGCAAGCGATTCGTGATGCAACAAGTATAGCCACGGTG
CCAGTGACGATAATTGATACCGATTTTGTCACGACCCAAGCGTTTTGTGAAGAGTACGAGGGCCGCACCCATCCTTTTGT
GGCTGCTTGTATCGATGAGTTTCGCTTAGACCATACAATTATGCTCGATAACAATGTGCCTTGGATTGATGATGGTATGC
GCTCTTTGGGTACACCTGAGGCACGTGGTCGTTTTGAGGCGCGTCTATTGGATATTTTCGCCCGTCATGATATTAAGCCG
CACATGATTGATCAACCAGATTATGAGGCGCGCTATCAGCAAGCATTGTTATTTATCGACCAGTATATTTATGGTCAAAA
GCCGTAA

Upstream 100 bases:

>100_bases
CGGGTAAGTATTCACGAACCTTTATACCATGCTTATAAATACTAAAAACTTTAGACAACTAAAAATAAATTGCACTCAGT
AAGTTTGAATAAGGACAGCT

Downstream 100 bases:

>100_bases
AAGATAACCCAAATTAAAAAAGCATCACTGACACTATTATTTATTTTAGGAATATAACGCCATGCGTATTAAGCTACTAG
ACAATATCACTGGGAAATGG

Product: nicotinamide-nucleotide adenylyltransferase

Products: NA

Alternate protein names: Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MYESGLMIGHFEPLHLGQMRSILNAAGQAKNLHLVIMTHPSPHPDFKITLQDKARWLQMACADLPFIHIHTTDEIELPVH
ESFADVTIDITASNAKLQHLIDALNLPAETVVFVAENHPLAQSDICKQLIMQVVTTPLQPDFDSYAIARNPVAYWSALHP
QARSNYTKTVAIVGGESSGKTTLVHKLANYYGASFALEMGRVYVGTDLGGTEIGLQYSDYGPIALHHAQAIRDATSIATV
PVTIIDTDFVTTQAFCEEYEGRTHPFVAACIDEFRLDHTIMLDNNVPWIDDGMRSLGTPEARGRFEARLLDIFARHDIKP
HMIDQPDYEARYQQALLFIDQYIYGQKP

Sequences:

>Translated_348_residues
MYESGLMIGHFEPLHLGQMRSILNAAGQAKNLHLVIMTHPSPHPDFKITLQDKARWLQMACADLPFIHIHTTDEIELPVH
ESFADVTIDITASNAKLQHLIDALNLPAETVVFVAENHPLAQSDICKQLIMQVVTTPLQPDFDSYAIARNPVAYWSALHP
QARSNYTKTVAIVGGESSGKTTLVHKLANYYGASFALEMGRVYVGTDLGGTEIGLQYSDYGPIALHHAQAIRDATSIATV
PVTIIDTDFVTTQAFCEEYEGRTHPFVAACIDEFRLDHTIMLDNNVPWIDDGMRSLGTPEARGRFEARLLDIFARHDIKP
HMIDQPDYEARYQQALLFIDQYIYGQKP
>Mature_348_residues
MYESGLMIGHFEPLHLGQMRSILNAAGQAKNLHLVIMTHPSPHPDFKITLQDKARWLQMACADLPFIHIHTTDEIELPVH
ESFADVTIDITASNAKLQHLIDALNLPAETVVFVAENHPLAQSDICKQLIMQVVTTPLQPDFDSYAIARNPVAYWSALHP
QARSNYTKTVAIVGGESSGKTTLVHKLANYYGASFALEMGRVYVGTDLGGTEIGLQYSDYGPIALHHAQAIRDATSIATV
PVTIIDTDFVTTQAFCEEYEGRTHPFVAACIDEFRLDHTIMLDNNVPWIDDGMRSLGTPEARGRFEARLLDIFARHDIKP
HMIDQPDYEARYQQALLFIDQYIYGQKP

Specific function: This enzyme has three activities:DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase

COG id: COG3172

COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082440, Length=358, Percent_Identity=36.3128491620112, Blast_Score=211, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016429
- InterPro:   IPR004821
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR006417
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: =2.7.7.1; =2.7.1.22 [H]

Molecular weight: Translated: 38910; Mature: 38910

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYESGLMIGHFEPLHLGQMRSILNAAGQAKNLHLVIMTHPSPHPDFKITLQDKARWLQMA
CCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHH
CADLPFIHIHTTDEIELPVHESFADVTIDITASNAKLQHLIDALNLPAETVVFVAENHPL
HHCCCEEEEECCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCC
AQSDICKQLIMQVVTTPLQPDFDSYAIARNPVAYWSALHPQARSNYTKTVAIVGGESSGK
CHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCHHHCCCEEEEEEEECCCCCC
TTLVHKLANYYGASFALEMGRVYVGTDLGGTEIGLQYSDYGPIALHHAQAIRDATSIATV
HHHHHHHHHHHCCCEEEEECEEEEECCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHEEE
PVTIIDTDFVTTQAFCEEYEGRTHPFVAACIDEFRLDHTIMLDNNVPWIDDGMRSLGTPE
EEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCC
ARGRFEARLLDIFARHDIKPHMIDQPDYEARYQQALLFIDQYIYGQKP
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYESGLMIGHFEPLHLGQMRSILNAAGQAKNLHLVIMTHPSPHPDFKITLQDKARWLQMA
CCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHH
CADLPFIHIHTTDEIELPVHESFADVTIDITASNAKLQHLIDALNLPAETVVFVAENHPL
HHCCCEEEEECCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCC
AQSDICKQLIMQVVTTPLQPDFDSYAIARNPVAYWSALHPQARSNYTKTVAIVGGESSGK
CHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCHHHCCCEEEEEEEECCCCCC
TTLVHKLANYYGASFALEMGRVYVGTDLGGTEIGLQYSDYGPIALHHAQAIRDATSIATV
HHHHHHHHHHHCCCEEEEECEEEEECCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHEEE
PVTIIDTDFVTTQAFCEEYEGRTHPFVAACIDEFRLDHTIMLDNNVPWIDDGMRSLGTPE
EEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCC
ARGRFEARLLDIFARHDIKPHMIDQPDYEARYQQALLFIDQYIYGQKP
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 1327967 [H]