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The map label for this gene is yafC [C]

Identifier: 93006764

GI number: 93006764

Start: 2393864

End: 2394730

Strand: Direct

Name: yafC [C]

Synonym: Pcryo_1940

Alternate gene names: 93006764

Gene position: 2393864-2394730 (Clockwise)

Preceding gene: 93006762

Following gene: 93006765

Centisome position: 78.23

GC content: 45.56

Gene sequence:

>867_bases
ATGAAGTGGGATGGTATCAGTGAGTTTGTTTACGTGGCAGAATTTGAGAGCTTTACTAAAGCGGCGTCCAAGCTTGCCAT
CTCGACGGCTCAAGTGAGTCGGCAAGTCACTGCACTGGAGAAGCGGCTCAATACCAAGCTGCTGTATCGAACCACGCGTA
AAGTGTCGCTAACAGAAGAAGGGCGGGTGTTTTATCAGCATTGCCGCAGCATCCTTGATGGACTCGATGCCGCAGAGCAA
GCGGTCAGTAATTTGCAGTCCAAACCGCAAGGTCGCATCAATCTGACAGCTCCTGTCACCTATGGAGAACAGCAGCTATT
GCCACTGATAAATGATTTTATGGTGCAATATAGTGATATCGAAGTGACGGCTTTTTTGAGCAATCAGAAGATTGATTTGA
TTGAAGGTGGTTATGATTTGGCAATACGTATCGGTAAGCTGAGCGACTCTACGATGATGGCAAATAAACTCAGTCACCGT
ACCAACTTTGTCTGTGCATCGCCCGCTTATTTTGAGAAATATGGTCGCCCAAAAACCTTGGATGAGCTGAGTCAACACAG
CTGCTTATTGGGTACACTTGATTATTGGCATTTTAAAGAAGCAGATACAGAGCGCAATATACGCGTGAGCGGCAAGATAC
GTTATAACAGTGGTTATAGCTTGGTTGATGCCGCGCTAAAGGGGCTTGGTATCGTACAATTGCCAGATTATTATGTACAA
AAACATCTAGCAACCGGTCGTTTAATGACGGTATTGGACGGATATAGAGAGCAAGAAGAAGGGATATGGGCAGTCTACCC
GCACAATCGTCATTTATCGCCAAAAATTAGGCTGTTGGTGGACTATCTAGCAGATCATTTGGCGTAA

Upstream 100 bases:

>100_bases
TTTAATGGTTGAATTTTAATAGTGTGATTATTTTATATTACTTCCCTGCTAATGATAATATAGCATATTTGCAAATATCT
TTTACACAGTGGTAATAATC

Downstream 100 bases:

>100_bases
TAATTCGTTAAGGCCTATTGCAATGCGACTCTTTACTATGCACCGTTTATTACGCACAATGTAATGATGTATTATAAGGA
CTCGATGTTGCCAACCATCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKWDGISEFVYVAEFESFTKAASKLAISTAQVSRQVTALEKRLNTKLLYRTTRKVSLTEEGRVFYQHCRSILDGLDAAEQ
AVSNLQSKPQGRINLTAPVTYGEQQLLPLINDFMVQYSDIEVTAFLSNQKIDLIEGGYDLAIRIGKLSDSTMMANKLSHR
TNFVCASPAYFEKYGRPKTLDELSQHSCLLGTLDYWHFKEADTERNIRVSGKIRYNSGYSLVDAALKGLGIVQLPDYYVQ
KHLATGRLMTVLDGYREQEEGIWAVYPHNRHLSPKIRLLVDYLADHLA

Sequences:

>Translated_288_residues
MKWDGISEFVYVAEFESFTKAASKLAISTAQVSRQVTALEKRLNTKLLYRTTRKVSLTEEGRVFYQHCRSILDGLDAAEQ
AVSNLQSKPQGRINLTAPVTYGEQQLLPLINDFMVQYSDIEVTAFLSNQKIDLIEGGYDLAIRIGKLSDSTMMANKLSHR
TNFVCASPAYFEKYGRPKTLDELSQHSCLLGTLDYWHFKEADTERNIRVSGKIRYNSGYSLVDAALKGLGIVQLPDYYVQ
KHLATGRLMTVLDGYREQEEGIWAVYPHNRHLSPKIRLLVDYLADHLA
>Mature_288_residues
MKWDGISEFVYVAEFESFTKAASKLAISTAQVSRQVTALEKRLNTKLLYRTTRKVSLTEEGRVFYQHCRSILDGLDAAEQ
AVSNLQSKPQGRINLTAPVTYGEQQLLPLINDFMVQYSDIEVTAFLSNQKIDLIEGGYDLAIRIGKLSDSTMMANKLSHR
TNFVCASPAYFEKYGRPKTLDELSQHSCLLGTLDYWHFKEADTERNIRVSGKIRYNSGYSLVDAALKGLGIVQLPDYYVQ
KHLATGRLMTVLDGYREQEEGIWAVYPHNRHLSPKIRLLVDYLADHLA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=37.0629370629371, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=32.8719723183391, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=32.7402135231317, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081978, Length=285, Percent_Identity=31.2280701754386, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=29.3515358361775, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=28.3737024221453, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789639, Length=286, Percent_Identity=29.020979020979, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788706, Length=252, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786448, Length=273, Percent_Identity=24.5421245421245, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790011, Length=259, Percent_Identity=26.6409266409266, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=26.8774703557312, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=24.1106719367589, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787879, Length=295, Percent_Identity=26.4406779661017, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI157672245, Length=128, Percent_Identity=32.03125, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=28.7671232876712, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32681; Mature: 32681

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWDGISEFVYVAEFESFTKAASKLAISTAQVSRQVTALEKRLNTKLLYRTTRKVSLTEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCEEEECCC
GRVFYQHCRSILDGLDAAEQAVSNLQSKPQGRINLTAPVTYGEQQLLPLINDFMVQYSDI
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHEECCE
EVTAFLSNQKIDLIEGGYDLAIRIGKLSDSTMMANKLSHRTNFVCASPAYFEKYGRPKTL
EEEEEECCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCHHHHHCCCCCCH
DELSQHSCLLGTLDYWHFKEADTERNIRVSGKIRYNSGYSLVDAALKGLGIVQLPDYYVQ
HHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCEEECCHHHHH
KHLATGRLMTVLDGYREQEEGIWAVYPHNRHLSPKIRLLVDYLADHLA
HHHHCCHHHHHHHCCHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKWDGISEFVYVAEFESFTKAASKLAISTAQVSRQVTALEKRLNTKLLYRTTRKVSLTEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCEEEECCC
GRVFYQHCRSILDGLDAAEQAVSNLQSKPQGRINLTAPVTYGEQQLLPLINDFMVQYSDI
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHEECCE
EVTAFLSNQKIDLIEGGYDLAIRIGKLSDSTMMANKLSHRTNFVCASPAYFEKYGRPKTL
EEEEEECCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCHHHHHCCCCCCH
DELSQHSCLLGTLDYWHFKEADTERNIRVSGKIRYNSGYSLVDAALKGLGIVQLPDYYVQ
HHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCEEECCHHHHH
KHLATGRLMTVLDGYREQEEGIWAVYPHNRHLSPKIRLLVDYLADHLA
HHHHCCHHHHHHHCCHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]