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The map label for this gene is pucI [H]

Identifier: 93006742

GI number: 93006742

Start: 2368739

End: 2370250

Strand: Direct

Name: pucI [H]

Synonym: Pcryo_1918

Alternate gene names: 93006742

Gene position: 2368739-2370250 (Clockwise)

Preceding gene: 93006741

Following gene: 93006743

Centisome position: 77.41

GC content: 43.85

Gene sequence:

>1512_bases
ATGTCCACTGCGAATGAGCAAGGAAGTCAGCTCAGTACTCAATCCAATAGCGCGCTCTATGATTGCAATACCTTTAATCC
AGAACGCAAAAACTCAAGATTATACAATGAAGATCTTGCCCCACTACAGCCTGAACAGCGTAATTGGGGCTGGTTTGCGA
TATTTAATGTTTGGTCCAATGATATTCAGAGCTTATTCGGGTATACCTTAGCGGCCTCCTTATTTTTGAGTTATGGACTG
AATGGGTGGTGGGTCATGGCAGCGATTATCTGCTCAGGATTCATCGTCATGCTGATGGTCAACTTAACTGGCAAACCCAG
TGTCAAATATGGCATCCCTTTTCCAGTCTTGATACGTGCCAGCATGGGTATCAATGGCGCTAACTTACCTGCCATCTTAC
GCGCTATTATCGGTATCTTTTGGTACGGGGTGCAGACCTACTTTGCCTCAACTGCAGTTGCCATTTTGTTGACTATTGTC
ATAGGTAGCCCAGATACGACATTTTTAGGCTTGAATAGTACCGCTTGGATTGCCTTTGTCATCGTTTGGGTATTTCAGAT
TTTACTGTTTTGGGCAGGCATCGAGCCGATCAAGCACTTTTTGAATTGGGCAGGACCGCTAGTCTATGTCGTTATGGTCA
TCTTGATGGCCGTCATCTGGTATCAATCAGGCTCTGAGTTGTTACCTGCGATTAACTCTATCTTCGCTAGTGGCAGCGAT
TATAGTGGAAATGCCATACAGGCATTTACTGCTATCGTCGGTACGATGGTTGCTTATTTCGCAGCAGTCGTCATAAACTT
CGGTGATTTCTCACGATTTTTGCGTAGTGAGCGCGATATGCGCTTGGGTAACTTACTCGGTTTGCCGATTAACATGGTAT
TTTTCTCGTTCATCGCCTTGGTCATTACCGCTGGAACCCTAGTAATATTTGGCGAAGCATTGACCAATCCATCGGATATT
GTTGATCGTGTAGATTCTCTACCCTTGACCATTATTGCAGCGCTGACCTTCTTTGCTGCAACCGTCGGTATCAATATGGT
TGCAAACTTTATTCCCCCTGCTTACGATTTGGCTAACTTATTTCCTAAGCATATTAACTTTCGCATGGGCGGTTTAATTA
CTGCTGTTATCGCCTTTTTCGTTGGTGCTTTGTGGCTATCATTTATCAGTAAGATCGGTATTGTCGGTTTTGTAAATACT
ATTGGTGCGGTTATCGCTCCATTCTTTGGTATCTTAGTCATCGATTATTACCTAATTAAACGTCAACATATCGATATGAA
TGACTTATTTTCCGCTCAACCAACTGGTGCTTATTACTACTACAAAGGCTGGAACATCCGTGCGCTTATCGCCTTTGCAA
TCGGCGCTTTATTCTCTATCGCCACGGTATTGATACCCGCACTGACTAAGCTTGAAGGTTATGGTTTCTTATTAGGCGCG
GCAATAGGTGGCATCGTGTATTGGGTATTGATGCGCCCTTATGCAGTAGATCCTGACACCATAGTAGAATAA

Upstream 100 bases:

>100_bases
CTTGACCAATGACCTAGACATCCTTAAAGTGTTAACAATAAACATTAATAAGTGTTAACAATATTTGTTTCGATTGATTT
CAATCTAAAGGGAGAAAAGG

Downstream 100 bases:

>100_bases
GGCAGTGAAGTCAGAAAGATGAAGTGCAATAGTAGAATAAAAAATAGTTATTACATTTCTAATAGATCTATTACTCTGTT
TTTATAAAGGATAAAACACC

Product: cytosine/purines, uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSTANEQGSQLSTQSNSALYDCNTFNPERKNSRLYNEDLAPLQPEQRNWGWFAIFNVWSNDIQSLFGYTLAASLFLSYGL
NGWWVMAAIICSGFIVMLMVNLTGKPSVKYGIPFPVLIRASMGINGANLPAILRAIIGIFWYGVQTYFASTAVAILLTIV
IGSPDTTFLGLNSTAWIAFVIVWVFQILLFWAGIEPIKHFLNWAGPLVYVVMVILMAVIWYQSGSELLPAINSIFASGSD
YSGNAIQAFTAIVGTMVAYFAAVVINFGDFSRFLRSERDMRLGNLLGLPINMVFFSFIALVITAGTLVIFGEALTNPSDI
VDRVDSLPLTIIAALTFFAATVGINMVANFIPPAYDLANLFPKHINFRMGGLITAVIAFFVGALWLSFISKIGIVGFVNT
IGAVIAPFFGILVIDYYLIKRQHIDMNDLFSAQPTGAYYYYKGWNIRALIAFAIGALFSIATVLIPALTKLEGYGFLLGA
AIGGIVYWVLMRPYAVDPDTIVE

Sequences:

>Translated_503_residues
MSTANEQGSQLSTQSNSALYDCNTFNPERKNSRLYNEDLAPLQPEQRNWGWFAIFNVWSNDIQSLFGYTLAASLFLSYGL
NGWWVMAAIICSGFIVMLMVNLTGKPSVKYGIPFPVLIRASMGINGANLPAILRAIIGIFWYGVQTYFASTAVAILLTIV
IGSPDTTFLGLNSTAWIAFVIVWVFQILLFWAGIEPIKHFLNWAGPLVYVVMVILMAVIWYQSGSELLPAINSIFASGSD
YSGNAIQAFTAIVGTMVAYFAAVVINFGDFSRFLRSERDMRLGNLLGLPINMVFFSFIALVITAGTLVIFGEALTNPSDI
VDRVDSLPLTIIAALTFFAATVGINMVANFIPPAYDLANLFPKHINFRMGGLITAVIAFFVGALWLSFISKIGIVGFVNT
IGAVIAPFFGILVIDYYLIKRQHIDMNDLFSAQPTGAYYYYKGWNIRALIAFAIGALFSIATVLIPALTKLEGYGFLLGA
AIGGIVYWVLMRPYAVDPDTIVE
>Mature_502_residues
STANEQGSQLSTQSNSALYDCNTFNPERKNSRLYNEDLAPLQPEQRNWGWFAIFNVWSNDIQSLFGYTLAASLFLSYGLN
GWWVMAAIICSGFIVMLMVNLTGKPSVKYGIPFPVLIRASMGINGANLPAILRAIIGIFWYGVQTYFASTAVAILLTIVI
GSPDTTFLGLNSTAWIAFVIVWVFQILLFWAGIEPIKHFLNWAGPLVYVVMVILMAVIWYQSGSELLPAINSIFASGSDY
SGNAIQAFTAIVGTMVAYFAAVVINFGDFSRFLRSERDMRLGNLLGLPINMVFFSFIALVITAGTLVIFGEALTNPSDIV
DRVDSLPLTIIAALTFFAATVGINMVANFIPPAYDLANLFPKHINFRMGGLITAVIAFFVGALWLSFISKIGIVGFVNTI
GAVIAPFFGILVIDYYLIKRQHIDMNDLFSAQPTGAYYYYKGWNIRALIAFAIGALFSIATVLIPALTKLEGYGFLLGAA
IGGIVYWVLMRPYAVDPDTIVE

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=474, Percent_Identity=29.1139240506329, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6322219, Length=500, Percent_Identity=26.8, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319495, Length=467, Percent_Identity=26.9807280513919, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319429, Length=469, Percent_Identity=23.6673773987207, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324766, Length=441, Percent_Identity=21.3151927437642, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323266, Length=441, Percent_Identity=20.4081632653061, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324645, Length=439, Percent_Identity=20.0455580865604, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 55193; Mature: 55062

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTANEQGSQLSTQSNSALYDCNTFNPERKNSRLYNEDLAPLQPEQRNWGWFAIFNVWSN
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHH
DIQSLFGYTLAASLFLSYGLNGWWVMAAIICSGFIVMLMVNLTGKPSVKYGIPFPVLIRA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHEE
SMGINGANLPAILRAIIGIFWYGVQTYFASTAVAILLTIVIGSPDTTFLGLNSTAWIAFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
IVWVFQILLFWAGIEPIKHFLNWAGPLVYVVMVILMAVIWYQSGSELLPAINSIFASGSD
HHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
YSGNAIQAFTAIVGTMVAYFAAVVINFGDFSRFLRSERDMRLGNLLGLPINMVFFSFIAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
VITAGTLVIFGEALTNPSDIVDRVDSLPLTIIAALTFFAATVGINMVANFIPPAYDLANL
HHHHHHHHEEHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPKHINFRMGGLITAVIAFFVGALWLSFISKIGIVGFVNTIGAVIAPFFGILVIDYYLIK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQHIDMNDLFSAQPTGAYYYYKGWNIRALIAFAIGALFSIATVLIPALTKLEGYGFLLGA
HHCCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIGGIVYWVLMRPYAVDPDTIVE
HHHHHHHHHHHCCCCCCCHHHCC
>Mature Secondary Structure 
STANEQGSQLSTQSNSALYDCNTFNPERKNSRLYNEDLAPLQPEQRNWGWFAIFNVWSN
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHH
DIQSLFGYTLAASLFLSYGLNGWWVMAAIICSGFIVMLMVNLTGKPSVKYGIPFPVLIRA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHEE
SMGINGANLPAILRAIIGIFWYGVQTYFASTAVAILLTIVIGSPDTTFLGLNSTAWIAFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
IVWVFQILLFWAGIEPIKHFLNWAGPLVYVVMVILMAVIWYQSGSELLPAINSIFASGSD
HHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
YSGNAIQAFTAIVGTMVAYFAAVVINFGDFSRFLRSERDMRLGNLLGLPINMVFFSFIAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
VITAGTLVIFGEALTNPSDIVDRVDSLPLTIIAALTFFAATVGINMVANFIPPAYDLANL
HHHHHHHHEEHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPKHINFRMGGLITAVIAFFVGALWLSFISKIGIVGFVNTIGAVIAPFFGILVIDYYLIK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQHIDMNDLFSAQPTGAYYYYKGWNIRALIAFAIGALFSIATVLIPALTKLEGYGFLLGA
HHCCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIGGIVYWVLMRPYAVDPDTIVE
HHHHHHHHHHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]