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The map label for this gene is glcD [H]

Identifier: 93006709

GI number: 93006709

Start: 2328549

End: 2330027

Strand: Direct

Name: glcD [H]

Synonym: Pcryo_1885

Alternate gene names: 93006709

Gene position: 2328549-2330027 (Clockwise)

Preceding gene: 93006708

Following gene: 93006710

Centisome position: 76.1

GC content: 49.76

Gene sequence:

>1479_bases
ATGAATCAGAGCCAAAGACCTGATAAAGCTGCTTTAGTAGAGCTATTTCGCGATTTTATTGAGCCTCAGTACGTGATCAA
TGATGAGGAGACTCAAAAGCCTTTTGAGTGTGATGGCTTATCGGTATATTGCGATATGCCACTCATTACGGTGCTCCCAA
AAACCGTTGAGCAAGTTTGTGAGGTCATGCGAATCTGTTATCGCTATCAAATCCCTGTGGTCGCACGCGGTGCCGGTACG
GGTTTGTGTGCAGGTGCAATGCCCAACCCTGATGGCGTCTTATTGGTGTTGTCACGATTCAAACATATCCTTGATATTGA
TCCACTTGCCCGTAGCGCACGTTTGCAGCCAGGTGTCCGTAACCTTGCTATTAGTGAGGCAGCTAGACAATATGGCTTGT
ATTATGGCCCAGACCCCTCCTCACAGATTGCCTGCTCCATCGGTGGCAACGTCGCCGAAAACTCAGGCGGCGTCCATTGT
CTCAAATATGGTCTGACCACTCACAATTTATTAAAAGTTGAGATGGTCACAGTCGAGGGGGATCTCATTACTATTGGTAG
TGAAGGTCTTGATAGCAACGGCTTTGATTTGATGGCTTTGATTACTGGCTCTGAGGGTCTACTTGGGGTGATTACTGAAG
TGACGGTCAAGCTGCTACCTAGCCCTGAAAAGGCTCAGGTCATTATGGCCGCTTTTGATAATGTCCAGACTGCAGGGGAT
GCGGTCGGCGGCGTGATTGCCAAAGGTATTATTCCAGCCGGACTTGAGATGATGGACAGCCCTGCCATCATAGCCGCCGA
AGCATTTGCTCATGCAGGCTATCCAATTGACGCCCAAGCTTTGCTGCTGTGTGAGTTAGATGGTAGCGAGGCGGAAGTTC
AAGAACAGATTGCAGAGGTTGAGCAGCTGTTTATCGAGCTTGGCGCAACCACAACACGTGTCTCACAAAGTGAGGCGGAG
CGCGCCTTATTGTGGAAAGGTCGAAAATCTGCATTCCCCGCTGTCGGACGAATATCCCCTGACTACTACTGCATGGATGG
CACCATTCCGCGTAGCCAACTTGCCCATGTCTTAACCGAAATGCAAAAACTCTCAGATGAATACGGTCTGCGCGTCGCCA
ACGTGTTTCATGCTGGGGACGGCAATTTGCACCCGCTTATCTTATTTGATGCCAATGTCCCAGGAGAGCTTGAGCGCACT
GAAGAGTTCGGTAGCCGGATTTTAGAGCTTTGCGTCAAGGTGGGCGGCTGCATAACTGGTGAGCATGGGGTTGGGGTCGA
AAAAATCCGCCAAATGACGATTCAGTTTAACGATCAAGAGCTGACACAATTTCATGCAATCAAGCACGCCTTTGACCCAA
TCGGTACGCTAAATCCAGGTAAGGGCATTCCAGTGCTCAAGCGCTGTCAAGAGTACCGTACCCTTGACAGAACGCAACAC
AACCATGACTCTCAACCACAGCAAGGAGATCTGGCATGA

Upstream 100 bases:

>100_bases
TATATTGATAGCTAATCTTTCCCTTTTTATTATAAGCTTAAGGTCATCTGTATAATAATGCAGTGAGTGTCGACAATTTA
GTGAGCAAAGGAATGCCACC

Downstream 100 bases:

>100_bases
CAGACATCAGTCAAGAGCTGATTGCGCGAGTAATGCAAGCCAGCCATGACGGCACAGCCCTACAAATCATGGGCGGGGGC
AGTAAGCAGTTTATGGGACG

Product: D-lactate dehydrogenase (cytochrome)

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNQSQRPDKAALVELFRDFIEPQYVINDEETQKPFECDGLSVYCDMPLITVLPKTVEQVCEVMRICYRYQIPVVARGAGT
GLCAGAMPNPDGVLLVLSRFKHILDIDPLARSARLQPGVRNLAISEAARQYGLYYGPDPSSQIACSIGGNVAENSGGVHC
LKYGLTTHNLLKVEMVTVEGDLITIGSEGLDSNGFDLMALITGSEGLLGVITEVTVKLLPSPEKAQVIMAAFDNVQTAGD
AVGGVIAKGIIPAGLEMMDSPAIIAAEAFAHAGYPIDAQALLLCELDGSEAEVQEQIAEVEQLFIELGATTTRVSQSEAE
RALLWKGRKSAFPAVGRISPDYYCMDGTIPRSQLAHVLTEMQKLSDEYGLRVANVFHAGDGNLHPLILFDANVPGELERT
EEFGSRILELCVKVGGCITGEHGVGVEKIRQMTIQFNDQELTQFHAIKHAFDPIGTLNPGKGIPVLKRCQEYRTLDRTQH
NHDSQPQQGDLA

Sequences:

>Translated_492_residues
MNQSQRPDKAALVELFRDFIEPQYVINDEETQKPFECDGLSVYCDMPLITVLPKTVEQVCEVMRICYRYQIPVVARGAGT
GLCAGAMPNPDGVLLVLSRFKHILDIDPLARSARLQPGVRNLAISEAARQYGLYYGPDPSSQIACSIGGNVAENSGGVHC
LKYGLTTHNLLKVEMVTVEGDLITIGSEGLDSNGFDLMALITGSEGLLGVITEVTVKLLPSPEKAQVIMAAFDNVQTAGD
AVGGVIAKGIIPAGLEMMDSPAIIAAEAFAHAGYPIDAQALLLCELDGSEAEVQEQIAEVEQLFIELGATTTRVSQSEAE
RALLWKGRKSAFPAVGRISPDYYCMDGTIPRSQLAHVLTEMQKLSDEYGLRVANVFHAGDGNLHPLILFDANVPGELERT
EEFGSRILELCVKVGGCITGEHGVGVEKIRQMTIQFNDQELTQFHAIKHAFDPIGTLNPGKGIPVLKRCQEYRTLDRTQH
NHDSQPQQGDLA
>Mature_492_residues
MNQSQRPDKAALVELFRDFIEPQYVINDEETQKPFECDGLSVYCDMPLITVLPKTVEQVCEVMRICYRYQIPVVARGAGT
GLCAGAMPNPDGVLLVLSRFKHILDIDPLARSARLQPGVRNLAISEAARQYGLYYGPDPSSQIACSIGGNVAENSGGVHC
LKYGLTTHNLLKVEMVTVEGDLITIGSEGLDSNGFDLMALITGSEGLLGVITEVTVKLLPSPEKAQVIMAAFDNVQTAGD
AVGGVIAKGIIPAGLEMMDSPAIIAAEAFAHAGYPIDAQALLLCELDGSEAEVQEQIAEVEQLFIELGATTTRVSQSEAE
RALLWKGRKSAFPAVGRISPDYYCMDGTIPRSQLAHVLTEMQKLSDEYGLRVANVFHAGDGNLHPLILFDANVPGELERT
EEFGSRILELCVKVGGCITGEHGVGVEKIRQMTIQFNDQELTQFHAIKHAFDPIGTLNPGKGIPVLKRCQEYRTLDRTQH
NHDSQPQQGDLA

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=461, Percent_Identity=29.0672451193059, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI37595754, Length=484, Percent_Identity=27.6859504132231, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI119964728, Length=452, Percent_Identity=26.5486725663717, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4501993, Length=303, Percent_Identity=24.4224422442244, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789351, Length=480, Percent_Identity=61.875, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI1787977, Length=543, Percent_Identity=23.9410681399632, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI48994907, Length=494, Percent_Identity=23.8866396761134, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71992373, Length=453, Percent_Identity=30.4635761589404, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71992381, Length=453, Percent_Identity=29.3598233995585, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71992392, Length=371, Percent_Identity=30.4582210242588, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17534361, Length=425, Percent_Identity=25.8823529411765, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556096, Length=284, Percent_Identity=22.5352112676056, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=429, Percent_Identity=26.5734265734266, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320023, Length=478, Percent_Identity=27.1966527196653, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6320764, Length=473, Percent_Identity=25.7928118393235, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18921117, Length=422, Percent_Identity=26.7772511848341, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24639277, Length=422, Percent_Identity=26.7772511848341, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24639275, Length=422, Percent_Identity=26.7772511848341, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24653753, Length=229, Percent_Identity=27.5109170305677, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53358; Mature: 53358

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSQRPDKAALVELFRDFIEPQYVINDEETQKPFECDGLSVYCDMPLITVLPKTVEQVC
CCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
EVMRICYRYQIPVVARGAGTGLCAGAMPNPDGVLLVLSRFKHILDIDPLARSARLQPGVR
HHHHHHHHHCCCEEECCCCCCEEECCCCCCCCEEHHHHHHHHHHCCCHHHHHHCCCCCHH
NLAISEAARQYGLYYGPDPSSQIACSIGGNVAENSGGVHCLKYGLTTHNLLKVEMVTVEG
HHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEC
DLITIGSEGLDSNGFDLMALITGSEGLLGVITEVTVKLLPSPEKAQVIMAAFDNVQTAGD
CEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHEEEEHHHCHHHHHH
AVGGVIAKGIIPAGLEMMDSPAIIAAEAFAHAGYPIDAQALLLCELDGSEAEVQEQIAEV
HHHHHHHHCCCCCCHHHCCCCCEEEHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHH
EQLFIELGATTTRVSQSEAERALLWKGRKSAFPAVGRISPDYYCMDGTIPRSQLAHVLTE
HHHHHHHCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
MQKLSDEYGLRVANVFHAGDGNLHPLILFDANVPGELERTEEFGSRILELCVKVGGCITG
HHHHHHHHCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEC
EHGVGVEKIRQMTIQFNDQELTQFHAIKHAFDPIGTLNPGKGIPVLKRCQEYRTLDRTQH
CCCCCHHHHHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
NHDSQPQQGDLA
CCCCCCCCCCCC
>Mature Secondary Structure
MNQSQRPDKAALVELFRDFIEPQYVINDEETQKPFECDGLSVYCDMPLITVLPKTVEQVC
CCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
EVMRICYRYQIPVVARGAGTGLCAGAMPNPDGVLLVLSRFKHILDIDPLARSARLQPGVR
HHHHHHHHHCCCEEECCCCCCEEECCCCCCCCEEHHHHHHHHHHCCCHHHHHHCCCCCHH
NLAISEAARQYGLYYGPDPSSQIACSIGGNVAENSGGVHCLKYGLTTHNLLKVEMVTVEG
HHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEC
DLITIGSEGLDSNGFDLMALITGSEGLLGVITEVTVKLLPSPEKAQVIMAAFDNVQTAGD
CEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHEEEEHHHCHHHHHH
AVGGVIAKGIIPAGLEMMDSPAIIAAEAFAHAGYPIDAQALLLCELDGSEAEVQEQIAEV
HHHHHHHHCCCCCCHHHCCCCCEEEHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHH
EQLFIELGATTTRVSQSEAERALLWKGRKSAFPAVGRISPDYYCMDGTIPRSQLAHVLTE
HHHHHHHCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
MQKLSDEYGLRVANVFHAGDGNLHPLILFDANVPGELERTEEFGSRILELCVKVGGCITG
HHHHHHHHCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEC
EHGVGVEKIRQMTIQFNDQELTQFHAIKHAFDPIGTLNPGKGIPVLKRCQEYRTLDRTQH
CCCCCHHHHHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
NHDSQPQQGDLA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]