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The map label for this gene is acnB [H]

Identifier: 93005576

GI number: 93005576

Start: 877536

End: 880139

Strand: Direct

Name: acnB [H]

Synonym: Pcryo_0746

Alternate gene names: 93005576

Gene position: 877536-880139 (Clockwise)

Preceding gene: 93005573

Following gene: 93005577

Centisome position: 28.68

GC content: 45.81

Gene sequence:

>2604_bases
GTGTTAGAAGCTTATCGTAAACATGTCGAAGAACGTGCTGAGCTAGGAACGGTTCCGCTACCACTGAACGAAAAACAAGT
AGCAGAGTTGGTTGAACTACTAAAGAATCCACCAGCGGGCGAAGATGCGTTCTTGATGGACTTGCTCGAAAATCGTATTC
CTGCCGGTGTTGACCAAGCTGCTTACGTTAAAGCGGCATTTTTGGCAGCGATCCTTAAGGGTGAAGCGACCTCTTCACTA
GTAAGTAAGAAAAAAGCGGTTCAAATCTTGGGTACGATGCAAGGTGGTTACAACGTTCAACCGCTAGTTGCTGCTCTTGA
TGATGCGGAAGTCGCGCAAGAAGCTGCCGATGCGTTGAAAAAAACCTTGCTAGTATTTGACGCATTCAATGATGTGACCG
AAAAAGCAGAAGCGGGCAATACTATTGCTAAAGAAGTCGTTCAATCTTGGGCTGACGCTGAGTGGTTCTTGAGCCGTGAC
GCTGTACCAGCAAAGACGACTTACACGACATTTAAAATCACTGGCGAAACCAATACCGATGATTTGTCTCCTGCACAAGA
TGCGTGGAGCCGTCCTGATATCCCATTACATTCATTAGCCATGCATAAAAACTCTCGCGACGGCATTACTGCTGACGAAG
AGGGCGTTATCGGTCCGATGAAACAGATCGAAGCGTTAAAAGAAAAAGGTCATCCATTAGCCTATGTTGGCGACGTGGTA
GGTACGGGTTCTAGCCGTAAATCTGCGACCAACTCAGTACTGTGGTTGATGGGTGATGATATCCCTAACGTGCCAAACAA
ACGTGGCGGCGGTCTAGTACTAGGTAATAATATTGCACCTATCTTCTTTAATACTATGGAAGATTCTGGCGCATTACCAA
TCGAAAAAGTAGCCGTTGATAACCTAAACATGGGCGATGTCTTTGATATCTATCCATATGATGGAAAAATCACTAAGCAT
GACTCTGATGAAGTACTGTCAACCTTCAAGCTAAACTCGCCAACTTTGCTTGATGAAGTTCGTGCTGGTGGTCGTATTCC
GTTAATCGTTGGTCGTGGTCTGACCAATCGTGCTCGTGAATATATGGGTCTTGGTCATTCAGACGTATTTGCTAAGCCAG
AAGAGCCAGTTGATACCGGCAAAGGCTTTACACTAGCGCAAAAAATGGTTGGTAAAGCTTGTGGTCTAGCAGGCGTTCGT
CCTGGTATGTACTGTGAACCAAAAATCACTACGGTTGGTAGTCAAGATACCACTGGTCCGATGACCCGTGATGAGCTAAA
AGACTTAGCCTGTCTAGGTTTCCAAGCAGATTTGGTGATGCAATCATTCTGTCATACGGCTGCTTATCCAAAGCCAGTTG
ACATCGAAACTCAGCATACACTACCTGACTTTATCATGAACCGTGGCGGCGTAAGCTTACGTCCAGGTGATGGTATTATT
CACTCGTGGTTGAACCGTATGCTACTTCCGGATACCGTTGGTACTGGTGGTGACTCGCATACGCGTTTCCCAATCGGTAT
CTCATTTCCAGCGGGTTCTGGTCTAGTAGCGTTTGCTGCTGCGACTGGTGTTATGCCACTTGATATGCCTGAATCGGTCC
GTGTCCGTTTCATCGGTGAGCGTCAAGCCGGTATCACCTTACGTGACTTAGTCCATGCAATCCCATACCAAGCGATTAAA
GAAGGTCTGTTAACCGTTGATAAGAAAGGTAAAATCAACGAGTTTAACGGTCGTATTCTTGAAATCGAAGGTTTAGAAGA
CTTAACCGCTGAGCAAGCGTTTGAATTATCTGATGCTTCAGCTGAGCGTAGTGCTGCTGGTTGTACGATTACTCTATCTG
AAAAGTCAGTTGCCGAGTATCTAACGTCGAACATCACATTATTAAAGTGGATGATTTCAGAAGGTTATGGCGATGCACGT
ACGATTAGCCGTCGTATCGAAGGTATGCAAGAGTGGTTGGCGAATCCAAGCCTGATGCGTGCTGATGAAGATGCAGAATA
TACCATCGATATGGTTATCGATATGTCTGAAATCAAAGAGCCGATTCTTTGCTGCCCGAACGATCCAGATGATGCAAAAA
CATTGGCTGATGTCGCTGGTGACACCATTGATGAAGTATTCATTGGTTCATGTATGACTAACATTGGTCACTTCCGCGCT
GCTGGTAAGTTGCTAAAAGATGTCCCAGCAGGTTCTTTGCAAACACGTCTATGGATTGCGCCGCCAACCAAAATGGACGC
GCGTCAGTTGATGGAAGAAGGTTACTATAATATCTATGCACAAGCCGGCGCTCGTACAGAGATGCCAGGTTGTTCATTAT
GTATGGGTAACCAAGCACGTATTGCACCGAATTCTACAGCCGTTTCGACTTCTACCCGTAACTTCCCGAACCGTTTAGGT
CAAGGTGCTAACGTATATCTAGCATCAGCTGAATTGGCGTCTGTTGCTGCGGTACTTGGTAAATTACCAACCAACGAAGA
GTATCAGCAGTACGCTGGCATGCTTGATAGCATGGGCGAAGAGGTGTATCAATACATGAACTTCGACCGTATGGAAGACT
ATACTGAAGAAGCTGATAAGATCAACGTTGCTCAGTTGAGCTAA

Upstream 100 bases:

>100_bases
GATTGCACGGATAATTATTCCATCTAGTATATAGACGCAGGCGGTCATGCTGACTACCCACGTAATGTGCTAATACTCCC
AACTGACGAGGACGACTATT

Downstream 100 bases:

>100_bases
TTTTGGTCAAATCTTAAATGCTTTGAAGCTTTACAATTATTGTTTAAATAGAAGCCCCGTATCGTCATGAACTGACCCCC
ATAAGTTGGACACAACTTTT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MLEAYRKHVEERAELGTVPLPLNEKQVAELVELLKNPPAGEDAFLMDLLENRIPAGVDQAAYVKAAFLAAILKGEATSSL
VSKKKAVQILGTMQGGYNVQPLVAALDDAEVAQEAADALKKTLLVFDAFNDVTEKAEAGNTIAKEVVQSWADAEWFLSRD
AVPAKTTYTTFKITGETNTDDLSPAQDAWSRPDIPLHSLAMHKNSRDGITADEEGVIGPMKQIEALKEKGHPLAYVGDVV
GTGSSRKSATNSVLWLMGDDIPNVPNKRGGGLVLGNNIAPIFFNTMEDSGALPIEKVAVDNLNMGDVFDIYPYDGKITKH
DSDEVLSTFKLNSPTLLDEVRAGGRIPLIVGRGLTNRAREYMGLGHSDVFAKPEEPVDTGKGFTLAQKMVGKACGLAGVR
PGMYCEPKITTVGSQDTTGPMTRDELKDLACLGFQADLVMQSFCHTAAYPKPVDIETQHTLPDFIMNRGGVSLRPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVRVRFIGERQAGITLRDLVHAIPYQAIK
EGLLTVDKKGKINEFNGRILEIEGLEDLTAEQAFELSDASAERSAAGCTITLSEKSVAEYLTSNITLLKWMISEGYGDAR
TISRRIEGMQEWLANPSLMRADEDAEYTIDMVIDMSEIKEPILCCPNDPDDAKTLADVAGDTIDEVFIGSCMTNIGHFRA
AGKLLKDVPAGSLQTRLWIAPPTKMDARQLMEEGYYNIYAQAGARTEMPGCSLCMGNQARIAPNSTAVSTSTRNFPNRLG
QGANVYLASAELASVAAVLGKLPTNEEYQQYAGMLDSMGEEVYQYMNFDRMEDYTEEADKINVAQLS

Sequences:

>Translated_867_residues
MLEAYRKHVEERAELGTVPLPLNEKQVAELVELLKNPPAGEDAFLMDLLENRIPAGVDQAAYVKAAFLAAILKGEATSSL
VSKKKAVQILGTMQGGYNVQPLVAALDDAEVAQEAADALKKTLLVFDAFNDVTEKAEAGNTIAKEVVQSWADAEWFLSRD
AVPAKTTYTTFKITGETNTDDLSPAQDAWSRPDIPLHSLAMHKNSRDGITADEEGVIGPMKQIEALKEKGHPLAYVGDVV
GTGSSRKSATNSVLWLMGDDIPNVPNKRGGGLVLGNNIAPIFFNTMEDSGALPIEKVAVDNLNMGDVFDIYPYDGKITKH
DSDEVLSTFKLNSPTLLDEVRAGGRIPLIVGRGLTNRAREYMGLGHSDVFAKPEEPVDTGKGFTLAQKMVGKACGLAGVR
PGMYCEPKITTVGSQDTTGPMTRDELKDLACLGFQADLVMQSFCHTAAYPKPVDIETQHTLPDFIMNRGGVSLRPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVRVRFIGERQAGITLRDLVHAIPYQAIK
EGLLTVDKKGKINEFNGRILEIEGLEDLTAEQAFELSDASAERSAAGCTITLSEKSVAEYLTSNITLLKWMISEGYGDAR
TISRRIEGMQEWLANPSLMRADEDAEYTIDMVIDMSEIKEPILCCPNDPDDAKTLADVAGDTIDEVFIGSCMTNIGHFRA
AGKLLKDVPAGSLQTRLWIAPPTKMDARQLMEEGYYNIYAQAGARTEMPGCSLCMGNQARIAPNSTAVSTSTRNFPNRLG
QGANVYLASAELASVAAVLGKLPTNEEYQQYAGMLDSMGEEVYQYMNFDRMEDYTEEADKINVAQLS
>Mature_867_residues
MLEAYRKHVEERAELGTVPLPLNEKQVAELVELLKNPPAGEDAFLMDLLENRIPAGVDQAAYVKAAFLAAILKGEATSSL
VSKKKAVQILGTMQGGYNVQPLVAALDDAEVAQEAADALKKTLLVFDAFNDVTEKAEAGNTIAKEVVQSWADAEWFLSRD
AVPAKTTYTTFKITGETNTDDLSPAQDAWSRPDIPLHSLAMHKNSRDGITADEEGVIGPMKQIEALKEKGHPLAYVGDVV
GTGSSRKSATNSVLWLMGDDIPNVPNKRGGGLVLGNNIAPIFFNTMEDSGALPIEKVAVDNLNMGDVFDIYPYDGKITKH
DSDEVLSTFKLNSPTLLDEVRAGGRIPLIVGRGLTNRAREYMGLGHSDVFAKPEEPVDTGKGFTLAQKMVGKACGLAGVR
PGMYCEPKITTVGSQDTTGPMTRDELKDLACLGFQADLVMQSFCHTAAYPKPVDIETQHTLPDFIMNRGGVSLRPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVRVRFIGERQAGITLRDLVHAIPYQAIK
EGLLTVDKKGKINEFNGRILEIEGLEDLTAEQAFELSDASAERSAAGCTITLSEKSVAEYLTSNITLLKWMISEGYGDAR
TISRRIEGMQEWLANPSLMRADEDAEYTIDMVIDMSEIKEPILCCPNDPDDAKTLADVAGDTIDEVFIGSCMTNIGHFRA
AGKLLKDVPAGSLQTRLWIAPPTKMDARQLMEEGYYNIYAQAGARTEMPGCSLCMGNQARIAPNSTAVSTSTRNFPNRLG
QGANVYLASAELASVAAVLGKLPTNEEYQQYAGMLDSMGEEVYQYMNFDRMEDYTEEADKINVAQLS

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=861, Percent_Identity=73.054587688734, Blast_Score=1293, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=391, Percent_Identity=25.5754475703325, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93918; Mature: 93918

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVEERAELGTVPLPLNEKQVAELVELLKNPPAGEDAFLMDLLENRIPAGVDQA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AYVKAAFLAAILKGEATSSLVSKKKAVQILGTMQGGYNVQPLVAALDDAEVAQEAADALK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHHH
KTLLVFDAFNDVTEKAEAGNTIAKEVVQSWADAEWFLSRDAVPAKTTYTTFKITGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCC
DLSPAQDAWSRPDIPLHSLAMHKNSRDGITADEEGVIGPMKQIEALKEKGHPLAYVGDVV
CCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECHHH
GTGSSRKSATNSVLWLMGDDIPNVPNKRGGGLVLGNNIAPIFFNTMEDSGALPIEKVAVD
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCHHHHEEC
NLNMGDVFDIYPYDGKITKHDSDEVLSTFKLNSPTLLDEVRAGGRIPLIVGRGLTNRARE
CCCCCCEEEECCCCCEEECCCCHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCHHHHHH
YMGLGHSDVFAKPEEPVDTGKGFTLAQKMVGKACGLAGVRPGMYCEPKITTVGSQDTTGP
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEECCCCCCCCC
MTRDELKDLACLGFQADLVMQSFCHTAAYPKPVDIETQHTLPDFIMNRGGVSLRPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVRVRFIGE
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCCCCCCEEEEEEC
RQAGITLRDLVHAIPYQAIKEGLLTVDKKGKINEFNGRILEIEGLEDLTAEQAFELSDAS
CCCCCCHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCC
AERSAAGCTITLSEKSVAEYLTSNITLLKWMISEGYGDARTISRRIEGMQEWLANPSLMR
CHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEE
ADEDAEYTIDMVIDMSEIKEPILCCPNDPDDAKTLADVAGDTIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEEEHHHHCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AGKLLKDVPAGSLQTRLWIAPPTKMDARQLMEEGYYNIYAQAGARTEMPGCSLCMGNQAR
HHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCCE
IAPNSTAVSTSTRNFPNRLGQGANVYLASAELASVAAVLGKLPTNEEYQQYAGMLDSMGE
ECCCCCEECCCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EVYQYMNFDRMEDYTEEADKINVAQLS
HHHHHHCHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MLEAYRKHVEERAELGTVPLPLNEKQVAELVELLKNPPAGEDAFLMDLLENRIPAGVDQA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AYVKAAFLAAILKGEATSSLVSKKKAVQILGTMQGGYNVQPLVAALDDAEVAQEAADALK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHHH
KTLLVFDAFNDVTEKAEAGNTIAKEVVQSWADAEWFLSRDAVPAKTTYTTFKITGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCC
DLSPAQDAWSRPDIPLHSLAMHKNSRDGITADEEGVIGPMKQIEALKEKGHPLAYVGDVV
CCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECHHH
GTGSSRKSATNSVLWLMGDDIPNVPNKRGGGLVLGNNIAPIFFNTMEDSGALPIEKVAVD
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCHHHHEEC
NLNMGDVFDIYPYDGKITKHDSDEVLSTFKLNSPTLLDEVRAGGRIPLIVGRGLTNRARE
CCCCCCEEEECCCCCEEECCCCHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCHHHHHH
YMGLGHSDVFAKPEEPVDTGKGFTLAQKMVGKACGLAGVRPGMYCEPKITTVGSQDTTGP
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEECCCCCCCCC
MTRDELKDLACLGFQADLVMQSFCHTAAYPKPVDIETQHTLPDFIMNRGGVSLRPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVRVRFIGE
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCCCCCCEEEEEEC
RQAGITLRDLVHAIPYQAIKEGLLTVDKKGKINEFNGRILEIEGLEDLTAEQAFELSDAS
CCCCCCHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCC
AERSAAGCTITLSEKSVAEYLTSNITLLKWMISEGYGDARTISRRIEGMQEWLANPSLMR
CHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEE
ADEDAEYTIDMVIDMSEIKEPILCCPNDPDDAKTLADVAGDTIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEEEHHHHCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AGKLLKDVPAGSLQTRLWIAPPTKMDARQLMEEGYYNIYAQAGARTEMPGCSLCMGNQAR
HHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCCE
IAPNSTAVSTSTRNFPNRLGQGANVYLASAELASVAAVLGKLPTNEEYQQYAGMLDSMGE
ECCCCCEECCCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EVYQYMNFDRMEDYTEEADKINVAQLS
HHHHHHCHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]