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The map label for this gene is recJ [H]

Identifier: 93005550

GI number: 93005550

Start: 848165

End: 849991

Strand: Direct

Name: recJ [H]

Synonym: Pcryo_0720

Alternate gene names: 93005550

Gene position: 848165-849991 (Clockwise)

Preceding gene: 93005534

Following gene: 93005557

Centisome position: 27.72

GC content: 46.52

Gene sequence:

>1827_bases
ATGCTAAATTTAACTCCCCGTTATACCAGCACTCGCATTGACGAGTTGCCCGCCGCGTTGCAACCACTTGCCGCCCAATC
ACTGACGCTTGCTCGCCTATATGCCGCTCGCGGTATTATGCAGCCAGATGAGCTTGAGATTCAGCTGGCTGGGTTGCTGC
CTGCTGAGAAGCTAAAAGGTATCATTGAAGCGGTAGATCTATTAGATATCGCTATTGATGACGGTCAGCGTATTCTGATC
GTTGGTGACTTTGACTGTGATGGAGCAACCAGTACGGCGTTAATGATGCGCGCCCTGACTGCCATGGGTGCCAAGGTTGA
TTTTCTAGTCCCTGACCGTTTTAAATACGGTTATGGGTTGACGCCAGAGATTGTCGAATTGGGTATTAAAACTTATCAAC
CGCAAATGATTGTCACGGTAGATAATGGTATCTCAAGCCATGAAGGAGTGGCGCGCGCGCAGGCTGATGGCATTACTGTC
ATTATCACTGACCATCATCTGACCACCAAAGAGACACCGCCTGCTGAAGCGGTAGTCAATCCCAATCAGCTTGATTGCAC
TTTTGCTAGTAAAGCTTTGGTAGGTGTTGGGGTAGCGTTTTACGTGCTCGGACGACTGGCAAAGCTGCGCCGCGATGCTG
GCAAATCTACCGTACAAGTCAGTCAATATTTGGATTTAGTGGCGCTTGGTACGATTGCTGATGTGGGCGTACTTGATAAA
AACAATCGGATCTTGGTCCATCATGGCTTAGCGGCTATTCGTCAAGGACGTGGTTCTCTTGGCATACTAGCGTTACTCGA
GCAAGCGGGTCGAGATCCCAAAAAACTACAGGCGCAAGATTTTGGTTTTGTCTTGGGTCCACGTATTAATGCGGCTGGGC
GTATGGATAATATGCGCATCGGTATTGAGTGCTTGCTAGCAGATGATTGGGGAACGGCGCAGCGATTGGCACAAGAGCTT
GAGCAGCTGAATCGTACGCGTCGCCAAGTAGAAGGTGAGATGCGTACGCAAGCGGATAATATTGTCCAAGCATTAGCTGC
TACTAATATAAGTATTGAAGCGGATGTTGAAGAAAGCGATGATATTATCGTTCAGAATAGGCTTGAGAAGAAGAGCACTA
ATACCCGCAGTATTGTGTTATATCAAGATGACTGGCATCAAGGTGTTATCGGTATCGTCGCAGGACGTTTGAAAGAAAGC
CATTATCTGCCGAGCATTGTCTTTGCGCCAGCGAATACTGAACGTACAGGTGCTGAAGATGCCATCAAAGGCTCAGCACG
TTCTATTGCTGGCGTGCATATTCGTGATGCCATTGAACAAGTTGCCGAGAGCCATCCTGATTTAATCAGTCATTTTGGCG
GTCATGCCATGGCAGCAGGACTGACGTTGAAAAGAGGTAACTTTGATGCCTTTGTGACAGCATTTAACACGGTACTGGCA
CAGATGGAGGATGAGGTATTCGCTGAGCAAAAATTCACCGATGGTGCTTTGCAAGCAAATGATTTTAGCTTATGGTTTGC
TGAGCATCTTGCAGAGTCTAGTATTTGGGGACATGGGTTTGCGCCACCGATATTTGATGGGGTGTTTGAAGTGTTGAACT
TTAAAGTGTTAAAGGATCAACACCTTAAACTGTCGCTACGTTATCCTGATGTAAAGTATCCGATTGAGGCGATTCAATTT
AATTTTGATAGCAGTGCTTGGGATTACCGTGCTGAAAAAGTACATATATTGTTTGAGCTGGACATCAATGAATGGAATGG
TAAACAAAGCTTGCAGTTAATGATAAAAGATTTGGCGCTGGTAAAAGAGACGGTCGAAGCGAACTAG

Upstream 100 bases:

>100_bases
ATGATGGATGTATAAGCAATCTCTAACCTTGCACCGACATGCCAAAAATTACCTCTAAATTAATATTGTGAGCACTTACT
AAAGCCAGTAGAATAGCTTC

Downstream 100 bases:

>100_bases
AGGCTTAACGGTACAGATATTAACTGCGCTAATAGTTGCTAAGCGTCCATATTTCCAATGCTTTAATATTTCCATTCAAT
GGTCGGAAACTGCCAATCAT

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MLNLTPRYTSTRIDELPAALQPLAAQSLTLARLYAARGIMQPDELEIQLAGLLPAEKLKGIIEAVDLLDIAIDDGQRILI
VGDFDCDGATSTALMMRALTAMGAKVDFLVPDRFKYGYGLTPEIVELGIKTYQPQMIVTVDNGISSHEGVARAQADGITV
IITDHHLTTKETPPAEAVVNPNQLDCTFASKALVGVGVAFYVLGRLAKLRRDAGKSTVQVSQYLDLVALGTIADVGVLDK
NNRILVHHGLAAIRQGRGSLGILALLEQAGRDPKKLQAQDFGFVLGPRINAAGRMDNMRIGIECLLADDWGTAQRLAQEL
EQLNRTRRQVEGEMRTQADNIVQALAATNISIEADVEESDDIIVQNRLEKKSTNTRSIVLYQDDWHQGVIGIVAGRLKES
HYLPSIVFAPANTERTGAEDAIKGSARSIAGVHIRDAIEQVAESHPDLISHFGGHAMAAGLTLKRGNFDAFVTAFNTVLA
QMEDEVFAEQKFTDGALQANDFSLWFAEHLAESSIWGHGFAPPIFDGVFEVLNFKVLKDQHLKLSLRYPDVKYPIEAIQF
NFDSSAWDYRAEKVHILFELDINEWNGKQSLQLMIKDLALVKETVEAN

Sequences:

>Translated_608_residues
MLNLTPRYTSTRIDELPAALQPLAAQSLTLARLYAARGIMQPDELEIQLAGLLPAEKLKGIIEAVDLLDIAIDDGQRILI
VGDFDCDGATSTALMMRALTAMGAKVDFLVPDRFKYGYGLTPEIVELGIKTYQPQMIVTVDNGISSHEGVARAQADGITV
IITDHHLTTKETPPAEAVVNPNQLDCTFASKALVGVGVAFYVLGRLAKLRRDAGKSTVQVSQYLDLVALGTIADVGVLDK
NNRILVHHGLAAIRQGRGSLGILALLEQAGRDPKKLQAQDFGFVLGPRINAAGRMDNMRIGIECLLADDWGTAQRLAQEL
EQLNRTRRQVEGEMRTQADNIVQALAATNISIEADVEESDDIIVQNRLEKKSTNTRSIVLYQDDWHQGVIGIVAGRLKES
HYLPSIVFAPANTERTGAEDAIKGSARSIAGVHIRDAIEQVAESHPDLISHFGGHAMAAGLTLKRGNFDAFVTAFNTVLA
QMEDEVFAEQKFTDGALQANDFSLWFAEHLAESSIWGHGFAPPIFDGVFEVLNFKVLKDQHLKLSLRYPDVKYPIEAIQF
NFDSSAWDYRAEKVHILFELDINEWNGKQSLQLMIKDLALVKETVEAN
>Mature_608_residues
MLNLTPRYTSTRIDELPAALQPLAAQSLTLARLYAARGIMQPDELEIQLAGLLPAEKLKGIIEAVDLLDIAIDDGQRILI
VGDFDCDGATSTALMMRALTAMGAKVDFLVPDRFKYGYGLTPEIVELGIKTYQPQMIVTVDNGISSHEGVARAQADGITV
IITDHHLTTKETPPAEAVVNPNQLDCTFASKALVGVGVAFYVLGRLAKLRRDAGKSTVQVSQYLDLVALGTIADVGVLDK
NNRILVHHGLAAIRQGRGSLGILALLEQAGRDPKKLQAQDFGFVLGPRINAAGRMDNMRIGIECLLADDWGTAQRLAQEL
EQLNRTRRQVEGEMRTQADNIVQALAATNISIEADVEESDDIIVQNRLEKKSTNTRSIVLYQDDWHQGVIGIVAGRLKES
HYLPSIVFAPANTERTGAEDAIKGSARSIAGVHIRDAIEQVAESHPDLISHFGGHAMAAGLTLKRGNFDAFVTAFNTVLA
QMEDEVFAEQKFTDGALQANDFSLWFAEHLAESSIWGHGFAPPIFDGVFEVLNFKVLKDQHLKLSLRYPDVKYPIEAIQF
NFDSSAWDYRAEKVHILFELDINEWNGKQSLQLMIKDLALVKETVEAN

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=593, Percent_Identity=42.6644182124789, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 66739; Mature: 66739

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLTPRYTSTRIDELPAALQPLAAQSLTLARLYAARGIMQPDELEIQLAGLLPAEKLKG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHH
IIEAVDLLDIAIDDGQRILIVGDFDCDGATSTALMMRALTAMGAKVDFLVPDRFKYGYGL
HHHHHHHHHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
TPEIVELGIKTYQPQMIVTVDNGISSHEGVARAQADGITVIITDHHLTTKETPPAEAVVN
CHHHHHHCHHCCCCEEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCEEEC
PNQLDCTFASKALVGVGVAFYVLGRLAKLRRDAGKSTVQVSQYLDLVALGTIADVGVLDK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCEECC
NNRILVHHGLAAIRQGRGSLGILALLEQAGRDPKKLQAQDFGFVLGPRINAAGRMDNMRI
CCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCCCCCCCCEEE
GIECLLADDWGTAQRLAQELEQLNRTRRQVEGEMRTQADNIVQALAATNISIEADVEESD
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
DIIVQNRLEKKSTNTRSIVLYQDDWHQGVIGIVAGRLKESHYLPSIVFAPANTERTGAED
CEEEHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
AIKGSARSIAGVHIRDAIEQVAESHPDLISHFGGHAMAAGLTLKRGNFDAFVTAFNTVLA
HHCCCCHHCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHCEEEECCCCHHHHHHHHHHHH
QMEDEVFAEQKFTDGALQANDFSLWFAEHLAESSIWGHGFAPPIFDGVFEVLNFKVLKDQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCEEEEECC
HLKLSLRYPDVKYPIEAIQFNFDSSAWDYRAEKVHILFELDINEWNGKQSLQLMIKDLAL
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHH
VKETVEAN
HHHHHCCC
>Mature Secondary Structure
MLNLTPRYTSTRIDELPAALQPLAAQSLTLARLYAARGIMQPDELEIQLAGLLPAEKLKG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHH
IIEAVDLLDIAIDDGQRILIVGDFDCDGATSTALMMRALTAMGAKVDFLVPDRFKYGYGL
HHHHHHHHHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
TPEIVELGIKTYQPQMIVTVDNGISSHEGVARAQADGITVIITDHHLTTKETPPAEAVVN
CHHHHHHCHHCCCCEEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCEEEC
PNQLDCTFASKALVGVGVAFYVLGRLAKLRRDAGKSTVQVSQYLDLVALGTIADVGVLDK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCEECC
NNRILVHHGLAAIRQGRGSLGILALLEQAGRDPKKLQAQDFGFVLGPRINAAGRMDNMRI
CCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCCCCCCCCEEE
GIECLLADDWGTAQRLAQELEQLNRTRRQVEGEMRTQADNIVQALAATNISIEADVEESD
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
DIIVQNRLEKKSTNTRSIVLYQDDWHQGVIGIVAGRLKESHYLPSIVFAPANTERTGAED
CEEEHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
AIKGSARSIAGVHIRDAIEQVAESHPDLISHFGGHAMAAGLTLKRGNFDAFVTAFNTVLA
HHCCCCHHCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHCEEEECCCCHHHHHHHHHHHH
QMEDEVFAEQKFTDGALQANDFSLWFAEHLAESSIWGHGFAPPIFDGVFEVLNFKVLKDQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCEEEEECC
HLKLSLRYPDVKYPIEAIQFNFDSSAWDYRAEKVHILFELDINEWNGKQSLQLMIKDLAL
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHH
VKETVEAN
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8168497 [H]